Sorbitol dehydrogenase as a part of Aspergillus niger D-galactose catabolism

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific


D-galactose is a hexose sugar found in hemicellulose. There are probably three different pathways for D-galactose catabolism in fungi, the Leloir, an oxidative and an oxidoreductive. The Leloir pathway has phosphorylated intermediates and galactokinase as the first enzyme. The oxidative pathway has no phosphorylated intermediates and D-galactose is oxidised to D-galactonate in the first reaction. In subsequent reactions D-galactonate is converted to pyruvate and glycerol. In addition to these two pathways an oxidoreductive pathway for D-galactose catabolism, that partly employs the enzymes of the L-arabinose pathway, was suggested to exist. In this work we studied the oxidoreductive D-galactose pathway where D-galactose is first reduced to
D-galactitol by a reductase that is also active with L-arabinose. It is then oxidised to L-xylo-3-hexulose by the L-arabitol dehydrogenase. It was suggested that L-xylo-3-hexulose is then reduced to D-sorbitol which is finally oxidised to D-fructose by a sorbitol dehydrogenase. We identified a gene coding
for a sorbitol dehydrogenase that is strongly induced on sorbitol and we evaluated the role of the enzyme in D-galactose catabolism.
Original languageEnglish
Title of host publication26th Fungal Genetics Conference at Asilomar March 15-20, 2011
PublisherGenetics Society of America
ChapterPoster Abstracts
Publication statusPublished - 2011
MoE publication typeNot Eligible
Event26th Fungal Genetics Conference - Asilomar, United States
Duration: 15 Mar 201120 Mar 2011

Publication series

SeriesFungal Genetics Reports


Conference26th Fungal Genetics Conference
CountryUnited States

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