Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

Outi M. Koivistoinen, Peter Richard, Merja Penttilä, Laura Ruohonen, Dominik Mojzita (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    18 Citations (Scopus)

    Abstract

    In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.

    Original languageEnglish
    Pages (from-to)378-383
    Number of pages6
    JournalFEBS Letters
    Volume586
    Issue number4
    DOIs
    Publication statusPublished - 17 Feb 2012
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Aspergillus niger
    • d-Galactose catabolism
    • d-Sorbitol
    • sdhA
    • Sorbitol dehydrogenase

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