Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

Outi M. Koivistoinen, Peter Richard, Merja Penttilä, Laura Ruohonen, Dominik Mojzita (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.

Original languageEnglish
Pages (from-to)378-383
Number of pages6
JournalFEBS Letters
Volume586
Issue number4
DOIs
Publication statusPublished - 17 Feb 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

L-Iditol 2-Dehydrogenase
Sorbitol
Aspergillus niger
Aspergillus
Galactose
Galactitol
Fructose
Oxidoreductases
Genes
Growth
Fungi
NAD
Oxidation
Enzymes

Keywords

  • Aspergillus niger
  • d-Galactose catabolism
  • d-Sorbitol
  • sdhA
  • Sorbitol dehydrogenase

Cite this

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title = "Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism",
abstract = "In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.",
keywords = "Aspergillus niger, d-Galactose catabolism, d-Sorbitol, sdhA, Sorbitol dehydrogenase",
author = "Koivistoinen, {Outi M.} and Peter Richard and Merja Penttil{\"a} and Laura Ruohonen and Dominik Mojzita",
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T1 - Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

AU - Koivistoinen, Outi M.

AU - Richard, Peter

AU - Penttilä, Merja

AU - Ruohonen, Laura

AU - Mojzita, Dominik

N1 - CA2: TK402 CA2: TK400 SDA: BIC ISI: BIOCHEMISTRY & MOLECULAR BIOLOGY

PY - 2012/2/17

Y1 - 2012/2/17

N2 - In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.

AB - In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.

KW - Aspergillus niger

KW - d-Galactose catabolism

KW - d-Sorbitol

KW - sdhA

KW - Sorbitol dehydrogenase

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