Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

Outi M. Koivistoinen, Peter Richard, Merja Penttilä, Laura Ruohonen, Dominik Mojzita (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.

Original languageEnglish
Pages (from-to)378-383
Number of pages6
JournalFEBS Letters
Volume586
Issue number4
DOIs
Publication statusPublished - 17 Feb 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • Aspergillus niger
  • d-Galactose catabolism
  • d-Sorbitol
  • sdhA
  • Sorbitol dehydrogenase

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