Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

Outi M. Koivistoinen; Peter Richard; Merja Penttilä; Laura Ruohonen; Dominik Mojzita

doi:10.1016/j.febslet.2012.01.004

Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

Outi M. Koivistoinen
, Peter Richard
, Merja Penttilä
, Laura Ruohonen
, Dominik Mojzita^*

^*Corresponding author for this work

BA5301 Production host engineering

VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

36 Citations (Scopus)

Abstract

In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with K_m of 50 ± 5 mM and v _max of 80 ± 10 U/mg.

Original language	English
Pages (from-to)	378-383
Journal	FEBS Letters
Volume	586
Issue number	4
DOIs	https://doi.org/10.1016/j.febslet.2012.01.004
Publication status	Published - 17 Feb 2012
MoE publication type	A1 Journal article-refereed

Funding

This work was financially supported by the Academy of Finland and is part of the research programme “White Biotechnology – Green Chemistry”, The Finnish Centre of Excellence Programme, 2008–2013, Project No. 118573.

Keywords

Aspergillus niger
d-Galactose catabolism
d-Sorbitol
sdhA
Sorbitol dehydrogenase

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10.1016/j.febslet.2012.01.004

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title = "Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism",

abstract = "In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.",

keywords = "Aspergillus niger, d-Galactose catabolism, d-Sorbitol, sdhA, Sorbitol dehydrogenase",

author = "Koivistoinen, \{Outi M.\} and Peter Richard and Merja Penttil{\"a} and Laura Ruohonen and Dominik Mojzita",

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T1 - Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

AU - Koivistoinen, Outi M.

AU - Richard, Peter

AU - Penttilä, Merja

AU - Ruohonen, Laura

AU - Mojzita, Dominik

N1 - CA2: TK402 CA2: TK400 SDA: BIC ISI: BIOCHEMISTRY & MOLECULAR BIOLOGY

PY - 2012/2/17

Y1 - 2012/2/17

N2 - In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.

AB - In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and v max of 80 ± 10 U/mg.

KW - Aspergillus niger

KW - d-Galactose catabolism

KW - d-Sorbitol

KW - sdhA

KW - Sorbitol dehydrogenase

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DO - 10.1016/j.febslet.2012.01.004

M3 - Article

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AN - SCOPUS:84857054968

SN - 0014-5793

VL - 586

SP - 378

EP - 383

JO - FEBS Letters

JF - FEBS Letters

IS - 4

ER -

Sorbitol dehydrogenase of Aspergillus niger, SdhA, is part of the oxido-reductive d-galactose pathway and essential for d-sorbitol catabolism

Abstract

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