Soxhlet extraction of mucic acid from fungal biomass

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Biologically produced mucic acid may form crystals before being separated from the biomass. Here we use Soxhlet extraction of fungal biomass to obtain mucic acid that was otherwise lost when separating biomass from the supernatant. Culture supernatant was used to extract the biomass, avoiding dilution of the mucic acid while retaining a cell-free suspension. Soxhlet extraction of biomass recovered 7% of total mucic acid produced at pH 4 and 24% at pH 6. The potential for mucic acid to cyclize to the 1,4-lactone was investigated by solid state NMR, confirming that very little lactone was present after the crystallization step.

Original languageEnglish
Pages (from-to)903-909
Number of pages7
JournalSeparation Science and Technology
Volume53
Issue number6
DOIs
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed

Fingerprint

Biomass
Acids
Lactones
Crystallization
Dilution
Suspensions
Nuclear magnetic resonance
galactaric acid
Crystals

Keywords

  • galactaric acid
  • Mucic acid
  • Soxhlet extraction

Cite this

@article{e4c0f971c17d4ad49ed132a2c95bd0b7,
title = "Soxhlet extraction of mucic acid from fungal biomass",
abstract = "Biologically produced mucic acid may form crystals before being separated from the biomass. Here we use Soxhlet extraction of fungal biomass to obtain mucic acid that was otherwise lost when separating biomass from the supernatant. Culture supernatant was used to extract the biomass, avoiding dilution of the mucic acid while retaining a cell-free suspension. Soxhlet extraction of biomass recovered 7{\%} of total mucic acid produced at pH 4 and 24{\%} at pH 6. The potential for mucic acid to cyclize to the 1,4-lactone was investigated by solid state NMR, confirming that very little lactone was present after the crystallization step.",
keywords = "galactaric acid, Mucic acid, Soxhlet extraction",
author = "Thomas, {David A.} and Tommi Virtanen and Wiebe, {Marilyn G.}",
year = "2018",
doi = "10.1080/01496395.2017.1407789",
language = "English",
volume = "53",
pages = "903--909",
journal = "Separation Science and Technology",
issn = "0149-6395",
publisher = "Taylor & Francis",
number = "6",

}

Soxhlet extraction of mucic acid from fungal biomass. / Thomas, David A.; Virtanen, Tommi; Wiebe, Marilyn G.

In: Separation Science and Technology, Vol. 53, No. 6, 2018, p. 903-909.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Soxhlet extraction of mucic acid from fungal biomass

AU - Thomas, David A.

AU - Virtanen, Tommi

AU - Wiebe, Marilyn G.

PY - 2018

Y1 - 2018

N2 - Biologically produced mucic acid may form crystals before being separated from the biomass. Here we use Soxhlet extraction of fungal biomass to obtain mucic acid that was otherwise lost when separating biomass from the supernatant. Culture supernatant was used to extract the biomass, avoiding dilution of the mucic acid while retaining a cell-free suspension. Soxhlet extraction of biomass recovered 7% of total mucic acid produced at pH 4 and 24% at pH 6. The potential for mucic acid to cyclize to the 1,4-lactone was investigated by solid state NMR, confirming that very little lactone was present after the crystallization step.

AB - Biologically produced mucic acid may form crystals before being separated from the biomass. Here we use Soxhlet extraction of fungal biomass to obtain mucic acid that was otherwise lost when separating biomass from the supernatant. Culture supernatant was used to extract the biomass, avoiding dilution of the mucic acid while retaining a cell-free suspension. Soxhlet extraction of biomass recovered 7% of total mucic acid produced at pH 4 and 24% at pH 6. The potential for mucic acid to cyclize to the 1,4-lactone was investigated by solid state NMR, confirming that very little lactone was present after the crystallization step.

KW - galactaric acid

KW - Mucic acid

KW - Soxhlet extraction

UR - http://www.scopus.com/inward/record.url?scp=85035139044&partnerID=8YFLogxK

U2 - 10.1080/01496395.2017.1407789

DO - 10.1080/01496395.2017.1407789

M3 - Article

VL - 53

SP - 903

EP - 909

JO - Separation Science and Technology

JF - Separation Science and Technology

SN - 0149-6395

IS - 6

ER -