Spatial distribution of glycerophospholipids in the ocular lens

Jaroslav Pól (Corresponding Author), Veronika Vidová, Tuulia Hyötyläinen, Michael Volný, Petr Novák, Martin Strohalm, Risto Kostiainen, Vladimír Havlícek, Susanne K. Wiedmer, Juha M. Holopainen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.
Original languageEnglish
Article numbere19441
Number of pages9
JournalPLoS ONE
Volume6
Issue number4
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Glycerophospholipids
eye lens
Crystalline Lens
Lens
Spatial distribution
Lenses
spatial distribution
Lens Diseases
Lipids
Phosphatidylethanolamines
Mass spectrometry
lipids
Phosphatidylcholines
phosphatidylethanolamines
Mass Spectrometry
phosphatidylcholines
Swine
Cyclotrons
Intraocular lenses
Phosphatidic Acids

Cite this

Pól, J., Vidová, V., Hyötyläinen, T., Volný, M., Novák, P., Strohalm, M., ... Holopainen, J. M. (2011). Spatial distribution of glycerophospholipids in the ocular lens. PLoS ONE, 6(4), [e19441]. https://doi.org/10.1371/journal.pone.0019441
Pól, Jaroslav ; Vidová, Veronika ; Hyötyläinen, Tuulia ; Volný, Michael ; Novák, Petr ; Strohalm, Martin ; Kostiainen, Risto ; Havlícek, Vladimír ; Wiedmer, Susanne K. ; Holopainen, Juha M. / Spatial distribution of glycerophospholipids in the ocular lens. In: PLoS ONE. 2011 ; Vol. 6, No. 4.
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title = "Spatial distribution of glycerophospholipids in the ocular lens",
abstract = "Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.",
author = "Jaroslav P{\'o}l and Veronika Vidov{\'a} and Tuulia Hy{\"o}tyl{\"a}inen and Michael Voln{\'y} and Petr Nov{\'a}k and Martin Strohalm and Risto Kostiainen and Vladim{\'i}r Havl{\'i}cek and Wiedmer, {Susanne K.} and Holopainen, {Juha M.}",
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Pól, J, Vidová, V, Hyötyläinen, T, Volný, M, Novák, P, Strohalm, M, Kostiainen, R, Havlícek, V, Wiedmer, SK & Holopainen, JM 2011, 'Spatial distribution of glycerophospholipids in the ocular lens', PLoS ONE, vol. 6, no. 4, e19441. https://doi.org/10.1371/journal.pone.0019441

Spatial distribution of glycerophospholipids in the ocular lens. / Pól, Jaroslav (Corresponding Author); Vidová, Veronika; Hyötyläinen, Tuulia; Volný, Michael; Novák, Petr; Strohalm, Martin; Kostiainen, Risto; Havlícek, Vladimír; Wiedmer, Susanne K.; Holopainen, Juha M. (Corresponding Author).

In: PLoS ONE, Vol. 6, No. 4, e19441, 2011.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Spatial distribution of glycerophospholipids in the ocular lens

AU - Pól, Jaroslav

AU - Vidová, Veronika

AU - Hyötyläinen, Tuulia

AU - Volný, Michael

AU - Novák, Petr

AU - Strohalm, Martin

AU - Kostiainen, Risto

AU - Havlícek, Vladimír

AU - Wiedmer, Susanne K.

AU - Holopainen, Juha M.

PY - 2011

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N2 - Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.

AB - Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.

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DO - 10.1371/journal.pone.0019441

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VL - 6

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JF - PLoS ONE

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ER -

Pól J, Vidová V, Hyötyläinen T, Volný M, Novák P, Strohalm M et al. Spatial distribution of glycerophospholipids in the ocular lens. PLoS ONE. 2011;6(4). e19441. https://doi.org/10.1371/journal.pone.0019441