Specific antibodies as a method for characterization of fiber surface chemistry

Arja Lappalainen, Maija Tenkanen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

2 Citations (Scopus)

Abstract

Wood fibers are composed mainly of cellulose, hemicellulose and lignin. Fiber properties such as bonding ability are largely governed by surface chemistry of fibers. FTIR and ESCA have been applied for analysis of wood fibers and papers, but these methods do not give information on the surface distribution of components. Microscopy offers a linkage between chemistry and structure with methods for distinctive and specific staining or labelling of fiber components. Antibodies can be used as specific markers for different carbohydrates. They have been produced against oligosaccharides obtained from xylan and xyloglucan (Takahashi and Sumiya, 1990, Sone and Sato, 1994). Also polysaccharides have been used as antigen (Moore, 1989, Knox et al., 1990, Westermark and Vennigerholz, 1995).
Original languageEnglish
Title of host publication2nd European Symposium on Enzymes in Grain Processing, ESEPG-2
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages219-222
ISBN (Electronic)951-38-5707-7
ISBN (Print)951-38-5706-9
Publication statusPublished - 2000
MoE publication typeA4 Article in a conference publication
EventEuropean Symposium on Enzymes in Grain Processing, ESEGP-2 - Helsinki, Finland
Duration: 8 Dec 199910 Dec 1999

Publication series

NameVTT Symposium
PublisherVTT
Number207
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Conference

ConferenceEuropean Symposium on Enzymes in Grain Processing, ESEGP-2
Abbreviated titleESEGP-2
CountryFinland
CityHelsinki
Period8/12/9910/12/99

Fingerprint

Surface chemistry
Antibodies
Fibers
Wood
Xylans
Lignin
Prednisone
Oligosaccharides
Cellulose
Labeling
Polysaccharides
Microscopic examination
Carbohydrates
Antigens

Cite this

Lappalainen, A., & Tenkanen, M. (2000). Specific antibodies as a method for characterization of fiber surface chemistry. In 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2 (pp. 219-222). Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 207
Lappalainen, Arja ; Tenkanen, Maija. / Specific antibodies as a method for characterization of fiber surface chemistry. 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. Espoo : VTT Technical Research Centre of Finland, 2000. pp. 219-222 (VTT Symposium; No. 207).
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Lappalainen, A & Tenkanen, M 2000, Specific antibodies as a method for characterization of fiber surface chemistry. in 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 207, pp. 219-222, European Symposium on Enzymes in Grain Processing, ESEGP-2, Helsinki, Finland, 8/12/99.

Specific antibodies as a method for characterization of fiber surface chemistry. / Lappalainen, Arja; Tenkanen, Maija.

2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. Espoo : VTT Technical Research Centre of Finland, 2000. p. 219-222 (VTT Symposium; No. 207).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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AU - Tenkanen, Maija

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AB - Wood fibers are composed mainly of cellulose, hemicellulose and lignin. Fiber properties such as bonding ability are largely governed by surface chemistry of fibers. FTIR and ESCA have been applied for analysis of wood fibers and papers, but these methods do not give information on the surface distribution of components. Microscopy offers a linkage between chemistry and structure with methods for distinctive and specific staining or labelling of fiber components. Antibodies can be used as specific markers for different carbohydrates. They have been produced against oligosaccharides obtained from xylan and xyloglucan (Takahashi and Sumiya, 1990, Sone and Sato, 1994). Also polysaccharides have been used as antigen (Moore, 1989, Knox et al., 1990, Westermark and Vennigerholz, 1995).

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Lappalainen A, Tenkanen M. Specific antibodies as a method for characterization of fiber surface chemistry. In 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. Espoo: VTT Technical Research Centre of Finland. 2000. p. 219-222. (VTT Symposium; No. 207).