Specific antibodies for immunochemical detection of wood-derived hemicelluloses

Arja Lappalainen, Maija Tenkanen, Jaakko Pere

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

    7 Citations (Scopus)

    Abstract

    Antibodies raised against wood-derived hemicelluloses can be used for chemical mapping of pulp fibres. Polyclonal antibodies were produced against cell wall carbohydrates, i.e. xylan, mannan and pectin, using both oligosaccharides coupled to BSA and polymeric carbohydrates as antigens. The antisera were checked for their specificity using ELISA techniques. In general, oligosaccharide-BSA conjugates were more antigenic and established higher affinities against the tested antigens as compared with the corresponding polymeric hemicelluloses. Applicability of the antisera was further tested on pulp fibres of known chemical composition. Labelling patterns of pulp fibres were in agreement with available information on the surface chemistry of fibres. FITC conjugate was shown to be suitable for delignified samples whereas AlexaFluor 633 label can also be used on lignin-containing material.
    Original languageEnglish
    Title of host publicationHemicelluloses: Science and Technology
    EditorsP. Gatenholm, M. Tenkanen
    PublisherAmerican Chemical Society ACS
    Pages140-156
    ISBN (Electronic)978-0-8412-1969-4
    ISBN (Print)0-8412-3842-1, 978-0-8412-3842-8
    DOIs
    Publication statusPublished - 2004
    MoE publication typeA4 Article in a conference publication
    Event1st International Symposium on Xylans, Mannans and Other Hemicelluloses - Orlando, United States
    Duration: 7 Apr 200211 Apr 2002

    Publication series

    SeriesACS Symposium Series
    Volume864
    ISSN0097-6156

    Conference

    Conference1st International Symposium on Xylans, Mannans and Other Hemicelluloses
    CountryUnited States
    CityOrlando
    Period7/04/0211/04/02

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  • Cite this

    Lappalainen, A., Tenkanen, M., & Pere, J. (2004). Specific antibodies for immunochemical detection of wood-derived hemicelluloses. In P. Gatenholm, & M. Tenkanen (Eds.), Hemicelluloses: Science and Technology (pp. 140-156). American Chemical Society ACS. ACS Symposium Series, Vol.. 864 https://doi.org/10.1021/bk-2004-0864.ch010