Specific role in sporulation of the yeast syntaxin Sso1p

Mattias Öyen, Jussi Jäntti, Sirkka Keränen, Hans Ronne

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

The yeast syntaxins Ssolp and Sso2p, acting at the cell membrane in the late secretion pathway, are highly homologous and have redundant functions in mitotically growing haploid and diploid cells. However, when diploid cells undergo sporulation, Ssolp is specifically required (1). This suggests the possible formation of a sporulation-specific complex where Sso2p cannot substitute for Ssolp. We have proceeded to study the role of Ssolp in sporulation. DAPI staining shows that meiosis is completed in sso1/sso1 mutant. The expression of Ssolp and Sso2p during sporulation was monitored in Western blots with antibodies specific for each protein. We found that the levels of both proteins remain constant throughout sporulation. In order to identify the part(s) of Ssolp that are specifically required during sporulation, a number of hybrid constructions between Ssolp and Sso2p were made and tested for their ability to complement the sporulation deficiency of the ssol/ssol strain. Our results suggest that the N-terminal part of Ssolp is particularly important for its sporulation-specific function. 1. Jäntti, J., Aalto. M., Öyen, NI., Sundqvist, L., Keränen, S. and Ronne, H. (2002). J. Cell Sci. 115, 409-420.
Original languageEnglish
Title of host publicationYeast 2003: the XXIst International Conference on Yeast Genetics and Molecular Biology
Subtitle of host publicationBook of abstracts
PublisherWiley
Publication statusPublished - 2003
MoE publication typeNot Eligible
Event21st International Conference on Yeast Genetics and Molecular Biology, Yeast 2003 - Gothenburg, Sweden
Duration: 7 Jul 200312 Jul 2003

Publication series

SeriesYeast
NumberS1
Volume20
ISSN0749-503X

Conference

Conference21st International Conference on Yeast Genetics and Molecular Biology, Yeast 2003
CountrySweden
CityGothenburg
Period7/07/0312/07/03

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    Öyen, M., Jäntti, J., Keränen, S., & Ronne, H. (2003). Specific role in sporulation of the yeast syntaxin Sso1p. In Yeast 2003: the XXIst International Conference on Yeast Genetics and Molecular Biology: Book of abstracts [S36] Wiley. Yeast, No. S1, Vol.. 20