SRB and methanogens in corrosion of steel in anaerobic water

    Research output: Contribution to conferenceConference articleScientificpeer-review

    Abstract

    During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.
    Original languageEnglish
    Publication statusPublished - 2014
    Event19th International Corrosion Congress, ICC 2014 - Jeju, Korea, Republic of
    Duration: 2 Nov 20146 Nov 2014
    Conference number: 19

    Conference

    Conference19th International Corrosion Congress, ICC 2014
    Abbreviated titleICC 2014
    CountryKorea, Republic of
    CityJeju
    Period2/11/146/11/14

    Fingerprint

    corrosion
    steel
    biofilm
    water
    targeting
    microbial community
    carbon
    decommissioning
    gene
    sulfate-reducing bacterium
    nuclear power plant
    repository
    scanning electron microscopy
    phylogenetics
    nutrient
    rate

    Keywords

    • MIC
    • steel
    • SRB
    • methanogenic archaea
    • ground water

    Cite this

    Rajala, P., Carpen, L., & Raulio, M. (2014). SRB and methanogens in corrosion of steel in anaerobic water. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.
    Rajala, Pauliina ; Carpen, Leena ; Raulio, Mari. / SRB and methanogens in corrosion of steel in anaerobic water. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.
    @conference{05fe872334b54b039e01d442533c1d75,
    title = "SRB and methanogens in corrosion of steel in anaerobic water",
    abstract = "During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.",
    keywords = "MIC, steel, SRB, methanogenic archaea, ground water",
    author = "Pauliina Rajala and Leena Carpen and Mari Raulio",
    note = "CA2: Ba2164; 19th International Corrosion Congress, ICC 2014, ICC 2014 ; Conference date: 02-11-2014 Through 06-11-2014",
    year = "2014",
    language = "English",

    }

    Rajala, P, Carpen, L & Raulio, M 2014, 'SRB and methanogens in corrosion of steel in anaerobic water', Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of, 2/11/14 - 6/11/14.

    SRB and methanogens in corrosion of steel in anaerobic water. / Rajala, Pauliina; Carpen, Leena; Raulio, Mari.

    2014. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.

    Research output: Contribution to conferenceConference articleScientificpeer-review

    TY - CONF

    T1 - SRB and methanogens in corrosion of steel in anaerobic water

    AU - Rajala, Pauliina

    AU - Carpen, Leena

    AU - Raulio, Mari

    N1 - CA2: Ba2164

    PY - 2014

    Y1 - 2014

    N2 - During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.

    AB - During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.

    KW - MIC

    KW - steel

    KW - SRB

    KW - methanogenic archaea

    KW - ground water

    M3 - Conference article

    ER -

    Rajala P, Carpen L, Raulio M. SRB and methanogens in corrosion of steel in anaerobic water. 2014. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.