SRB and methanogens in corrosion of steel in anaerobic water

Research output: Contribution to conferenceConference articleScientificpeer-review

Abstract

During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.
Original languageEnglish
Publication statusPublished - 2014
Event19th International Corrosion Congress, ICC 2014 - Jeju, Korea, Republic of
Duration: 2 Nov 20146 Nov 2014
Conference number: 19

Conference

Conference19th International Corrosion Congress, ICC 2014
Abbreviated titleICC 2014
CountryKorea, Republic of
CityJeju
Period2/11/146/11/14

Fingerprint

corrosion
steel
biofilm
water
targeting
microbial community
carbon
decommissioning
gene
sulfate-reducing bacterium
nuclear power plant
repository
scanning electron microscopy
phylogenetics
nutrient
rate

Keywords

  • MIC
  • steel
  • SRB
  • methanogenic archaea
  • ground water

Cite this

Rajala, P., Carpen, L., & Raulio, M. (2014). SRB and methanogens in corrosion of steel in anaerobic water. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.
Rajala, Pauliina ; Carpen, Leena ; Raulio, Mari. / SRB and methanogens in corrosion of steel in anaerobic water. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.
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title = "SRB and methanogens in corrosion of steel in anaerobic water",
abstract = "During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.",
keywords = "MIC, steel, SRB, methanogenic archaea, ground water",
author = "Pauliina Rajala and Leena Carpen and Mari Raulio",
note = "CA2: Ba2164; 19th International Corrosion Congress, ICC 2014, ICC 2014 ; Conference date: 02-11-2014 Through 06-11-2014",
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language = "English",

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Rajala, P, Carpen, L & Raulio, M 2014, 'SRB and methanogens in corrosion of steel in anaerobic water' Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of, 2/11/14 - 6/11/14, .

SRB and methanogens in corrosion of steel in anaerobic water. / Rajala, Pauliina; Carpen, Leena; Raulio, Mari.

2014. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.

Research output: Contribution to conferenceConference articleScientificpeer-review

TY - CONF

T1 - SRB and methanogens in corrosion of steel in anaerobic water

AU - Rajala, Pauliina

AU - Carpen, Leena

AU - Raulio, Mari

N1 - CA2: Ba2164

PY - 2014

Y1 - 2014

N2 - During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.

AB - During the operation, maintenance and decommissioning of nuclear power plants contaminated waste is produced. This waste is disposed of in underground repository 60-100 meters below the ground surface. The metallic waste consists mostly of carbon steel and stainless steel. A long-term field exposure show high corrosion rates, general corrosion up to 29 ?m a-1 and localized corrosion even higher. Corrosion rates this high are possible if microbes are present and produce corrosive products or alter the local microenvironment to corrosion favoring. The bacterial and archaeal composition of biofilm formed on the surface of carbon steel was studied using 16S rRNA gene targeting pyro-sequencing followed by phylogenetic analyses of microbial community. The functional potential of microbial communities in biofilm was studied by functional gene targeting quantitative PCR. The corrosion rate was analyzed with weigh loss measurements and the deposits on the surfaces were analyzed with SEM/EDS. Our results demonstrate that the bacterial diversity on the surface of carbon steel and their functionality is vast. Our results suggest that the role of methanogenic archaea in corrosive biofilm, in addition to sulphate reducing bacteria, could be greater than previously suspected in these nutrient poor conditions.

KW - MIC

KW - steel

KW - SRB

KW - methanogenic archaea

KW - ground water

M3 - Conference article

ER -

Rajala P, Carpen L, Raulio M. SRB and methanogens in corrosion of steel in anaerobic water. 2014. Paper presented at 19th International Corrosion Congress, ICC 2014, Jeju, Korea, Republic of.