Stable production of recombinant proteins in filamentous fungi: Problems and improvements

Marilyn Wiebe (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

Filamentous fungi such as Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei are able to produce and secrete large concentrations of enzymes (e.g. amylases, proteases, cellulases) into the environment. Increasingly, these species and a few others are being used to produce recombinant proteins, particularly proteins of fungal origin (Table 1). For producing recombinant proteins (which may be a protein which the host organism already makes, i.e. an homologous protein, or a new, i.e. heterologous, protein), filamentous fungi offer the advantages of possessing an efficient secretion system, being able to glycosylate proteins and having higher specific growth rates than plant, insect or mammalian cells. Although the filamentous growth form causes more difficulties for mixing and aeration than does the unicellular growth form of yeast and bacteria, efficient fermentation technologies have been developed for antibiotic, organic acid and native enzyme production from filamentous fungi.
Original languageEnglish
Pages (from-to)140-144
Number of pages5
JournalMycologist
Volume17
Issue number3
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Keywords

  • filamentous fungi
  • recombinant proteins
  • strain stability
  • heterokaryosis

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