Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.