Stable transformation of barley tissue culture by particle bombardment

Anneli Ritala, Leena Mannonen, Kristian Aspegren, Marjatta Salmenkallio-Marttila, Ulrika Kurten, Riitta Hannus, Mendez Lozano, Teemu Teeri, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

45 Citations (Scopus)

Abstract

Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

Original languageEnglish
Pages (from-to)435 - 440
Number of pages6
JournalPlant Cell Reports
Volume12
Issue number7-8
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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kanamycin kinase
tissue culture
barley
Southern blotting
Hordeum vulgare
cell suspension culture
plasmids
hybridization
antibiotics
genes

Cite this

Ritala, A., Mannonen, L., Aspegren, K., Salmenkallio-Marttila, M., Kurten, U., Hannus, R., ... Kauppinen, V. (1993). Stable transformation of barley tissue culture by particle bombardment. Plant Cell Reports, 12(7-8), 435 - 440. https://doi.org/10.1007/BF00234708
Ritala, Anneli ; Mannonen, Leena ; Aspegren, Kristian ; Salmenkallio-Marttila, Marjatta ; Kurten, Ulrika ; Hannus, Riitta ; Lozano, Mendez ; Teeri, Teemu ; Kauppinen, Veli. / Stable transformation of barley tissue culture by particle bombardment. In: Plant Cell Reports. 1993 ; Vol. 12, No. 7-8. pp. 435 - 440.
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abstract = "Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96{\%} of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40{\%} of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.",
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Ritala, A, Mannonen, L, Aspegren, K, Salmenkallio-Marttila, M, Kurten, U, Hannus, R, Lozano, M, Teeri, T & Kauppinen, V 1993, 'Stable transformation of barley tissue culture by particle bombardment', Plant Cell Reports, vol. 12, no. 7-8, pp. 435 - 440. https://doi.org/10.1007/BF00234708

Stable transformation of barley tissue culture by particle bombardment. / Ritala, Anneli; Mannonen, Leena; Aspegren, Kristian; Salmenkallio-Marttila, Marjatta; Kurten, Ulrika; Hannus, Riitta; Lozano, Mendez; Teeri, Teemu; Kauppinen, Veli.

In: Plant Cell Reports, Vol. 12, No. 7-8, 1993, p. 435 - 440.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Stable transformation of barley tissue culture by particle bombardment

AU - Ritala, Anneli

AU - Mannonen, Leena

AU - Aspegren, Kristian

AU - Salmenkallio-Marttila, Marjatta

AU - Kurten, Ulrika

AU - Hannus, Riitta

AU - Lozano, Mendez

AU - Teeri, Teemu

AU - Kauppinen, Veli

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PY - 1993

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N2 - Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

AB - Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

U2 - 10.1007/BF00234708

DO - 10.1007/BF00234708

M3 - Article

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SP - 435

EP - 440

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 7-8

ER -

Ritala A, Mannonen L, Aspegren K, Salmenkallio-Marttila M, Kurten U, Hannus R et al. Stable transformation of barley tissue culture by particle bombardment. Plant Cell Reports. 1993;12(7-8):435 - 440. https://doi.org/10.1007/BF00234708