Stable transformation of barley tissue culture by particle bombardment

Anneli Ritala, Leena Mannonen, Kristian Aspegren, Marjatta Salmenkallio-Marttila, Ulrika Kurten, Riitta Hannus, Mendez Lozano, Teemu Teeri, Veli Kauppinen

    Research output: Contribution to journalArticleScientificpeer-review

    45 Citations (Scopus)


    Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

    Original languageEnglish
    Pages (from-to)435 - 440
    Number of pages6
    JournalPlant Cell Reports
    Issue number7-8
    Publication statusPublished - 1993
    MoE publication typeA1 Journal article-refereed


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