Stable transformation of barley tissue culture by particle bombardment

Anneli Ritala, Leena Mannonen, Kristian Aspegren, Marjatta Salmenkallio-Marttila, Ulrika Kurten, Riitta Hannus, Mendez Lozano, Teemu Teeri, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

45 Citations (Scopus)

Abstract

Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

Original languageEnglish
Pages (from-to)435 - 440
Number of pages6
JournalPlant Cell Reports
Volume12
Issue number7-8
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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