Stable transformation of barley tissue culture by particle bombardment

Anneli Ritala; Leena Mannonen; Kristian Aspegren; Marjatta Salmenkallio-Marttila; Ulrika Kurten; Riitta Hannus; Mendez Lozano; Teemu Teeri; Veli Kauppinen

doi:10.1007/BF00234708

Stable transformation of barley tissue culture by particle bombardment

Anneli Ritala, Leena Mannonen, Kristian Aspegren, Marjatta Salmenkallio-Marttila, Ulrika Kurten, Riitta Hannus, Mendez Lozano, Teemu Teeri, Veli Kauppinen

University of Helsinki

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Abstract

Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic Geneticin^R. Enzymatic activity was tested in the Geneticin^R resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

Original language	English
Pages (from-to)	435 - 440
Number of pages	6
Journal	Plant Cell Reports
Volume	12
Issue number	7-8
DOIs	https://doi.org/10.1007/BF00234708
Publication status	Published - 1993
MoE publication type	A1 Journal article-refereed

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title = "Stable transformation of barley tissue culture by particle bombardment",

abstract = "Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.",

author = "Anneli Ritala and Leena Mannonen and Kristian Aspegren and Marjatta Salmenkallio-Marttila and Ulrika Kurten and Riitta Hannus and Mendez Lozano and Teemu Teeri and Veli Kauppinen",

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T1 - Stable transformation of barley tissue culture by particle bombardment

AU - Ritala, Anneli

AU - Mannonen, Leena

AU - Aspegren, Kristian

AU - Salmenkallio-Marttila, Marjatta

AU - Kurten, Ulrika

AU - Hannus, Riitta

AU - Lozano, Mendez

AU - Teeri, Teemu

AU - Kauppinen, Veli

N1 - Project code: BIO2025

PY - 1993

Y1 - 1993

N2 - Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

AB - Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

U2 - 10.1007/BF00234708

DO - 10.1007/BF00234708

M3 - Article

SN - 0721-7714

VL - 12

SP - 435

EP - 440

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 7-8

ER -

Stable transformation of barley tissue culture by particle bombardment

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