Standards for plant synthetic biology: A common syntax for exchange of DNA parts

N. J. Patron (Corresponding Author), D., Orzaez, S. Marillonnet, H. Warzecha, C. Matthewman, M. Youles, O. Raitskin, Leveau, G. Farré, C. Rogers, A. Smith, J. Hibberd, A. A. R. Webb, J. Locke, S. Schornack, J. Ajioka, D. C. Baulcombe, C. Zipfel, S. Kamoun, J. D. G. JonesH. Kuhn, S. Robatzek, H. P. Van Esse, D. Sanders, G. Oldroyd, C. Martin, R. Field, S. O'Connor, S. Fox, B. Wulff, B. Miller, A. Breakspear, G. Radhakrishnan, P.-M. Delaux, D. Loqué, A. Granell, A. Tissier, P. Shih, T. P. Brutnell, W.P. Quick, H. Rischer, P. D. Fraser, A. Aharoni, C. Raines, P. F. South, J.-M. Ané, B. R. Hamberger, J. Langdale, J. Stougaard, H. Bouwmeester

Research output: Contribution to journalArticleScientificpeer-review

79 Citations (Scopus)

Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.
Original languageEnglish
Pages (from-to)13-19
JournalNew Phytologist
Volume208
Issue number1
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Synthetic Biology
synthetic biology
plant biology
engineering
Inventors
bioengineering
DNA
restriction endonucleases
standardization
electronics
molecular cloning
nucleotide sequences
Bioengineering
DNA Restriction Enzymes
Organism Cloning
methodology

Keywords

  • cloning
  • DNA assembly
  • genetic syntax
  • GoldenGate
  • synthetic biology
  • Type IIS restriction endonucleases

Cite this

Patron, N. J., Orzaez, D. ., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., ... Bouwmeester, H. (2015). Standards for plant synthetic biology: A common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. https://doi.org/10.1111/nph.13532
Patron, N. J. ; Orzaez, D., ; Marillonnet, S. ; Warzecha, H. ; Matthewman, C. ; Youles, M. ; Raitskin, O. ; Leveau ; Farré, G. ; Rogers, C. ; Smith, A. ; Hibberd, J. ; Webb, A. A. R. ; Locke, J. ; Schornack, S. ; Ajioka, J. ; Baulcombe, D. C. ; Zipfel, C. ; Kamoun, S. ; Jones, J. D. G. ; Kuhn, H. ; Robatzek, S. ; Van Esse, H. P. ; Sanders, D. ; Oldroyd, G. ; Martin, C. ; Field, R. ; O'Connor, S. ; Fox, S. ; Wulff, B. ; Miller, B. ; Breakspear, A. ; Radhakrishnan, G. ; Delaux, P.-M. ; Loqué, D. ; Granell, A. ; Tissier, A. ; Shih, P. ; Brutnell, T. P. ; Quick, W.P. ; Rischer, H. ; Fraser, P. D. ; Aharoni, A. ; Raines, C. ; South, P. F. ; Ané, J.-M. ; Hamberger, B. R. ; Langdale, J. ; Stougaard, J. ; Bouwmeester, H. / Standards for plant synthetic biology : A common syntax for exchange of DNA parts. In: New Phytologist. 2015 ; Vol. 208, No. 1. pp. 13-19.
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Patron, NJ, Orzaez, D, Marillonnet, S, Warzecha, H, Matthewman, C, Youles, M, Raitskin, O, Leveau, Farré, G, Rogers, C, Smith, A, Hibberd, J, Webb, AAR, Locke, J, Schornack, S, Ajioka, J, Baulcombe, DC, Zipfel, C, Kamoun, S, Jones, JDG, Kuhn, H, Robatzek, S, Van Esse, HP, Sanders, D, Oldroyd, G, Martin, C, Field, R, O'Connor, S, Fox, S, Wulff, B, Miller, B, Breakspear, A, Radhakrishnan, G, Delaux, P-M, Loqué, D, Granell, A, Tissier, A, Shih, P, Brutnell, TP, Quick, WP, Rischer, H, Fraser, PD, Aharoni, A, Raines, C, South, PF, Ané, J-M, Hamberger, BR, Langdale, J, Stougaard, J & Bouwmeester, H 2015, 'Standards for plant synthetic biology: A common syntax for exchange of DNA parts', New Phytologist, vol. 208, no. 1, pp. 13-19. https://doi.org/10.1111/nph.13532

Standards for plant synthetic biology : A common syntax for exchange of DNA parts. / Patron, N. J. (Corresponding Author); Orzaez, D.,; Marillonnet, S.; Warzecha, H.; Matthewman, C.; Youles, M.; Raitskin, O.; Leveau; Farré, G.; Rogers, C.; Smith, A.; Hibberd, J.; Webb, A. A. R.; Locke, J.; Schornack, S.; Ajioka, J.; Baulcombe, D. C.; Zipfel, C.; Kamoun, S.; Jones, J. D. G.; Kuhn, H.; Robatzek, S.; Van Esse, H. P.; Sanders, D.; Oldroyd, G.; Martin, C.; Field, R.; O'Connor, S.; Fox, S.; Wulff, B.; Miller, B.; Breakspear, A.; Radhakrishnan, G.; Delaux, P.-M.; Loqué, D.; Granell, A.; Tissier, A.; Shih, P.; Brutnell, T. P.; Quick, W.P.; Rischer, H.; Fraser, P. D.; Aharoni, A.; Raines, C.; South, P. F.; Ané, J.-M.; Hamberger, B. R.; Langdale, J.; Stougaard, J.; Bouwmeester, H.

In: New Phytologist, Vol. 208, No. 1, 2015, p. 13-19.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Standards for plant synthetic biology

T2 - A common syntax for exchange of DNA parts

AU - Patron, N. J.

AU - Orzaez, D.,

AU - Marillonnet, S.

AU - Warzecha, H.

AU - Matthewman, C.

AU - Youles, M.

AU - Raitskin, O.

AU - Leveau, null

AU - Farré, G.

AU - Rogers, C.

AU - Smith, A.

AU - Hibberd, J.

AU - Webb, A. A. R.

AU - Locke, J.

AU - Schornack, S.

AU - Ajioka, J.

AU - Baulcombe, D. C.

AU - Zipfel, C.

AU - Kamoun, S.

AU - Jones, J. D. G.

AU - Kuhn, H.

AU - Robatzek, S.

AU - Van Esse, H. P.

AU - Sanders, D.

AU - Oldroyd, G.

AU - Martin, C.

AU - Field, R.

AU - O'Connor, S.

AU - Fox, S.

AU - Wulff, B.

AU - Miller, B.

AU - Breakspear, A.

AU - Radhakrishnan, G.

AU - Delaux, P.-M.

AU - Loqué, D.

AU - Granell, A.

AU - Tissier, A.

AU - Shih, P.

AU - Brutnell, T. P.

AU - Quick, W.P.

AU - Rischer, H.

AU - Fraser, P. D.

AU - Aharoni, A.

AU - Raines, C.

AU - South, P. F.

AU - Ané, J.-M.

AU - Hamberger, B. R.

AU - Langdale, J.

AU - Stougaard, J.

AU - Bouwmeester, H.

PY - 2015

Y1 - 2015

N2 - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

AB - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

KW - cloning

KW - DNA assembly

KW - genetic syntax

KW - GoldenGate

KW - synthetic biology

KW - Type IIS restriction endonucleases

U2 - 10.1111/nph.13532

DO - 10.1111/nph.13532

M3 - Article

VL - 208

SP - 13

EP - 19

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -

Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M et al. Standards for plant synthetic biology: A common syntax for exchange of DNA parts. New Phytologist. 2015;208(1):13-19. https://doi.org/10.1111/nph.13532