Standards for plant synthetic biology: A common syntax for exchange of DNA parts

N. J. Patron (Corresponding Author), D., Orzaez, S. Marillonnet, H. Warzecha, C. Matthewman, M. Youles, O. Raitskin, Leveau, G. Farré, C. Rogers, A. Smith, J. Hibberd, A. A. R. Webb, J. Locke, S. Schornack, J. Ajioka, D. C. Baulcombe, C. Zipfel, S. Kamoun, J. D. G. JonesH. Kuhn, S. Robatzek, H. P. Van Esse, D. Sanders, G. Oldroyd, C. Martin, R. Field, S. O'Connor, S. Fox, B. Wulff, B. Miller, A. Breakspear, G. Radhakrishnan, P.-M. Delaux, D. Loqué, A. Granell, A. Tissier, P. Shih, T. P. Brutnell, W.P. Quick, H. Rischer, P. D. Fraser, A. Aharoni, C. Raines, P. F. South, J.-M. Ané, B. R. Hamberger, J. Langdale, J. Stougaard, H. Bouwmeester

    Research output: Contribution to journalArticleScientificpeer-review

    124 Citations (Scopus)

    Abstract

    Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.
    Original languageEnglish
    Pages (from-to)13-19
    JournalNew Phytologist
    Volume208
    Issue number1
    DOIs
    Publication statusPublished - 2015
    MoE publication typeA1 Journal article-refereed

    Keywords

    • cloning
    • DNA assembly
    • genetic syntax
    • GoldenGate
    • synthetic biology
    • Type IIS restriction endonucleases

    Fingerprint

    Dive into the research topics of 'Standards for plant synthetic biology: A common syntax for exchange of DNA parts'. Together they form a unique fingerprint.

    Cite this