Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Nicola J. Patron; D., Orzaez; S. Marillonnet; H. Warzecha; C. Matthewman; M. Youles; O. Raitskin; Aymeric Leveau; G. Farré; C. Rogers; A. Smith; J. Hibberd; A.A.R. Webb; J. Locke; S. Schornack; J. Ajioka; D. C. Baulcombe; C. Zipfel; S. Kamoun; J. D. G. Jones; H. Kuhn; S. Robatzek; H. P. Van Esse; D. Sanders; G. Oldroyd; C. Martin; R. Field; S. O'Connor; S. Fox; B. Wulff; B. Miller; A. Breakspear; G. Radhakrishnan; P.-M. Delaux; D. Loqué; A. Granell; A. Tissier; P. Shih; T. P. Brutnell; W.P. Quick; Heiko Rischer; P. D. Fraser; A. Aharoni; C. Raines; P. F. South; J.-M. Ané; B. R. Hamberger; J. Langdale; J. Stougaard; H. Bouwmeester

doi:10.1111/nph.13532

Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Nicola J. Patron (Corresponding Author), D., Orzaez, S. Marillonnet, H. Warzecha, C. Matthewman, M. Youles, O. Raitskin, Aymeric Leveau, G. Farré, C. Rogers, A. Smith, J. Hibberd, A.A.R. Webb, J. Locke, S. Schornack, J. Ajioka, D. C. Baulcombe, C. Zipfel, S. Kamoun, J. D. G. JonesH. Kuhn, S. Robatzek, H. P. Van Esse, D. Sanders, G. Oldroyd, C. Martin, R. Field, S. O'Connor, S. Fox, B. Wulff, B. Miller, A. Breakspear, G. Radhakrishnan, P.-M. Delaux, D. Loqué, A. Granell, A. Tissier, P. Shih, T. P. Brutnell, W.P. Quick, Heiko Rischer, P. D. Fraser, A. Aharoni, C. Raines, P. F. South, J.-M. Ané, B. R. Hamberger, J. Langdale, J. Stougaard, H. Bouwmeester

BA5306 Plant biotechnology

Research output: Contribution to journal › Article › Scientific › peer-review

167 Citations (Scopus)

Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Original language	English
Pages (from-to)	13-19
Journal	New Phytologist
Volume	208
Issue number	1
DOIs	https://doi.org/10.1111/nph.13532
Publication status	Published - 2015
MoE publication type	A1 Journal article-refereed

Keywords

cloning
DNA assembly
genetic syntax
GoldenGate
synthetic biology
Type IIS restriction endonucleases

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10.1111/nph.13532

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Patron, N. J., Orzaez, D. ., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., Raitskin, O., Leveau, A., Farré, G., Rogers, C., Smith, A., Hibberd, J., Webb, A. A. R., Locke, J., Schornack, S., Ajioka, J., Baulcombe, D. C., Zipfel, C., Kamoun, S., ... Bouwmeester, H. (2015). Standards for plant synthetic biology: A common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. https://doi.org/10.1111/nph.13532

@article{be672e77377840c08ca7e0625c844e0d,

title = "Standards for plant synthetic biology: A common syntax for exchange of DNA parts",

abstract = "Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.",

keywords = "cloning, DNA assembly, genetic syntax, GoldenGate, synthetic biology, Type IIS restriction endonucleases",

author = "Patron, {Nicola J.} and D., Orzaez and S. Marillonnet and H. Warzecha and C. Matthewman and M. Youles and O. Raitskin and Aymeric Leveau and G. Farr{\'e} and C. Rogers and A. Smith and J. Hibberd and A.A.R. Webb and J. Locke and S. Schornack and J. Ajioka and Baulcombe, {D. C.} and C. Zipfel and S. Kamoun and Jones, {J. D. G.} and H. Kuhn and S. Robatzek and {Van Esse}, {H. P.} and D. Sanders and G. Oldroyd and C. Martin and R. Field and S. O'Connor and S. Fox and B. Wulff and B. Miller and A. Breakspear and G. Radhakrishnan and P.-M. Delaux and D. Loqu{\'e} and A. Granell and A. Tissier and P. Shih and Brutnell, {T. P.} and W.P. Quick and Heiko Rischer and Fraser, {P. D.} and A. Aharoni and C. Raines and South, {P. F.} and J.-M. An{\'e} and Hamberger, {B. R.} and J. Langdale and J. Stougaard and H. Bouwmeester",

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doi = "10.1111/nph.13532",

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Patron, NJ, Orzaez, D, Marillonnet, S, Warzecha, H, Matthewman, C, Youles, M, Raitskin, O, Leveau, A, Farré, G, Rogers, C, Smith, A, Hibberd, J, Webb, AAR, Locke, J, Schornack, S, Ajioka, J, Baulcombe, DC, Zipfel, C, Kamoun, S, Jones, JDG, Kuhn, H, Robatzek, S, Van Esse, HP, Sanders, D, Oldroyd, G, Martin, C, Field, R, O'Connor, S, Fox, S, Wulff, B, Miller, B, Breakspear, A, Radhakrishnan, G, Delaux, P-M, Loqué, D, Granell, A, Tissier, A, Shih, P, Brutnell, TP, Quick, WP, Rischer, H, Fraser, PD, Aharoni, A, Raines, C, South, PF, Ané, J-M, Hamberger, BR, Langdale, J, Stougaard, J & Bouwmeester, H 2015, 'Standards for plant synthetic biology: A common syntax for exchange of DNA parts', New Phytologist, vol. 208, no. 1, pp. 13-19. https://doi.org/10.1111/nph.13532

TY - JOUR

T1 - Standards for plant synthetic biology

T2 - A common syntax for exchange of DNA parts

AU - Patron, Nicola J.

AU - Orzaez, D.,

AU - Marillonnet, S.

AU - Warzecha, H.

AU - Matthewman, C.

AU - Youles, M.

AU - Raitskin, O.

AU - Leveau, Aymeric

AU - Farré, G.

AU - Rogers, C.

AU - Smith, A.

AU - Hibberd, J.

AU - Webb, A.A.R.

AU - Locke, J.

AU - Schornack, S.

AU - Ajioka, J.

AU - Baulcombe, D. C.

AU - Zipfel, C.

AU - Kamoun, S.

AU - Jones, J. D. G.

AU - Kuhn, H.

AU - Robatzek, S.

AU - Van Esse, H. P.

AU - Sanders, D.

AU - Oldroyd, G.

AU - Martin, C.

AU - Field, R.

AU - O'Connor, S.

AU - Fox, S.

AU - Wulff, B.

AU - Miller, B.

AU - Breakspear, A.

AU - Radhakrishnan, G.

AU - Delaux, P.-M.

AU - Loqué, D.

AU - Granell, A.

AU - Tissier, A.

AU - Shih, P.

AU - Brutnell, T. P.

AU - Quick, W.P.

AU - Rischer, Heiko

AU - Fraser, P. D.

AU - Aharoni, A.

AU - Raines, C.

AU - South, P. F.

AU - Ané, J.-M.

AU - Hamberger, B. R.

AU - Langdale, J.

AU - Stougaard, J.

AU - Bouwmeester, H.

PY - 2015

Y1 - 2015

N2 - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

AB - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

KW - cloning

KW - DNA assembly

KW - genetic syntax

KW - GoldenGate

KW - synthetic biology

KW - Type IIS restriction endonucleases

U2 - 10.1111/nph.13532

DO - 10.1111/nph.13532

M3 - Article

SN - 0028-646X

VL - 208

SP - 13

EP - 19

JO - New Phytologist

JF - New Phytologist

IS - 1

ER -

Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Abstract

Keywords

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