Starch acetate microparticles for drug delivery into retinal pigment epithelium: In vitro study

Laura Tuovinen (Corresponding Author), Eija Ruhanen, Tarja Kinnarinen, Seppo Rönkkö, Jukka Pelkonen, Arto Urtti, Soili Peltonen, Kristiina Järvinen

Research output: Contribution to journalArticleScientificpeer-review

30 Citations (Scopus)

Abstract

Starch acetates are novel biodegradable polymers which undergo slower degradation and swelling than native starch. Retinal pigment epithelium (RPE) is an important target tissue in ocular treatment. The cellular uptake of starch acetate microparticles and degradation of starch acetate by cultured human RPE-cell line (D407) was examined. Calcein-containing starch acetate microparticles were prepared by a modified water-in-oil-in-water double-emulsion technique. The cellular uptake of the starch acetate microparticles was analysed using flow cytometry and confocal microscopy. Degradation of starch acetate films by the homogenate of lysed RPE cells was determined by gel permeation chromatography. The effect of the microparticles on RPE cell viability was determined by the MTT colorimetric assay. The mean diameter (D50%) of microparticles was 11 μm. During 3-h incubation in RPE-cell culture, 8.1±0.8% of D407 cells took up starch acetate microparticles. Confocal microscopy confirmed the internalisation of microparticles. Incubation of the starch acetate film in the RPE-cell homogenate considerably decreased the molecular weight of starch acetate in the film during 24 h. The viability of cultured RPE cells was at least 82% after 24-h incubation with the microparticles. The present results show that the starch acetate microparticles are taken up by the RPE cells and the polymer can be degraded by the enzymes in these cells. Starch acetate microparticles may be suitable for drug delivery to the RPE.
Original languageEnglish
Pages (from-to)407-413
JournalJournal of Controlled Release
Volume98
Issue number3
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Retinal Pigment Epithelium
Pharmaceutical Preparations
Confocal Microscopy
Polymers
In Vitro Techniques
starch acetate
Water
Emulsions
Starch
Gel Chromatography
Cell Survival
Flow Cytometry
Oils
Cell Culture Techniques
Molecular Weight
Cell Line

Keywords

  • starch acetate
  • microparticles
  • retinal pigment epithelium
  • cellular uptake
  • enzymatic degradation
  • biopolymers

Cite this

Tuovinen, L., Ruhanen, E., Kinnarinen, T., Rönkkö, S., Pelkonen, J., Urtti, A., ... Järvinen, K. (2004). Starch acetate microparticles for drug delivery into retinal pigment epithelium: In vitro study. Journal of Controlled Release, 98(3), 407-413. https://doi.org/10.1016/j.jconrel.2004.05.016
Tuovinen, Laura ; Ruhanen, Eija ; Kinnarinen, Tarja ; Rönkkö, Seppo ; Pelkonen, Jukka ; Urtti, Arto ; Peltonen, Soili ; Järvinen, Kristiina. / Starch acetate microparticles for drug delivery into retinal pigment epithelium : In vitro study. In: Journal of Controlled Release. 2004 ; Vol. 98, No. 3. pp. 407-413.
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abstract = "Starch acetates are novel biodegradable polymers which undergo slower degradation and swelling than native starch. Retinal pigment epithelium (RPE) is an important target tissue in ocular treatment. The cellular uptake of starch acetate microparticles and degradation of starch acetate by cultured human RPE-cell line (D407) was examined. Calcein-containing starch acetate microparticles were prepared by a modified water-in-oil-in-water double-emulsion technique. The cellular uptake of the starch acetate microparticles was analysed using flow cytometry and confocal microscopy. Degradation of starch acetate films by the homogenate of lysed RPE cells was determined by gel permeation chromatography. The effect of the microparticles on RPE cell viability was determined by the MTT colorimetric assay. The mean diameter (D50{\%}) of microparticles was 11 μm. During 3-h incubation in RPE-cell culture, 8.1±0.8{\%} of D407 cells took up starch acetate microparticles. Confocal microscopy confirmed the internalisation of microparticles. Incubation of the starch acetate film in the RPE-cell homogenate considerably decreased the molecular weight of starch acetate in the film during 24 h. The viability of cultured RPE cells was at least 82{\%} after 24-h incubation with the microparticles. The present results show that the starch acetate microparticles are taken up by the RPE cells and the polymer can be degraded by the enzymes in these cells. Starch acetate microparticles may be suitable for drug delivery to the RPE.",
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Tuovinen, L, Ruhanen, E, Kinnarinen, T, Rönkkö, S, Pelkonen, J, Urtti, A, Peltonen, S & Järvinen, K 2004, 'Starch acetate microparticles for drug delivery into retinal pigment epithelium: In vitro study', Journal of Controlled Release, vol. 98, no. 3, pp. 407-413. https://doi.org/10.1016/j.jconrel.2004.05.016

Starch acetate microparticles for drug delivery into retinal pigment epithelium : In vitro study. / Tuovinen, Laura (Corresponding Author); Ruhanen, Eija; Kinnarinen, Tarja; Rönkkö, Seppo; Pelkonen, Jukka; Urtti, Arto; Peltonen, Soili; Järvinen, Kristiina.

In: Journal of Controlled Release, Vol. 98, No. 3, 2004, p. 407-413.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Starch acetate microparticles for drug delivery into retinal pigment epithelium

T2 - In vitro study

AU - Tuovinen, Laura

AU - Ruhanen, Eija

AU - Kinnarinen, Tarja

AU - Rönkkö, Seppo

AU - Pelkonen, Jukka

AU - Urtti, Arto

AU - Peltonen, Soili

AU - Järvinen, Kristiina

PY - 2004

Y1 - 2004

N2 - Starch acetates are novel biodegradable polymers which undergo slower degradation and swelling than native starch. Retinal pigment epithelium (RPE) is an important target tissue in ocular treatment. The cellular uptake of starch acetate microparticles and degradation of starch acetate by cultured human RPE-cell line (D407) was examined. Calcein-containing starch acetate microparticles were prepared by a modified water-in-oil-in-water double-emulsion technique. The cellular uptake of the starch acetate microparticles was analysed using flow cytometry and confocal microscopy. Degradation of starch acetate films by the homogenate of lysed RPE cells was determined by gel permeation chromatography. The effect of the microparticles on RPE cell viability was determined by the MTT colorimetric assay. The mean diameter (D50%) of microparticles was 11 μm. During 3-h incubation in RPE-cell culture, 8.1±0.8% of D407 cells took up starch acetate microparticles. Confocal microscopy confirmed the internalisation of microparticles. Incubation of the starch acetate film in the RPE-cell homogenate considerably decreased the molecular weight of starch acetate in the film during 24 h. The viability of cultured RPE cells was at least 82% after 24-h incubation with the microparticles. The present results show that the starch acetate microparticles are taken up by the RPE cells and the polymer can be degraded by the enzymes in these cells. Starch acetate microparticles may be suitable for drug delivery to the RPE.

AB - Starch acetates are novel biodegradable polymers which undergo slower degradation and swelling than native starch. Retinal pigment epithelium (RPE) is an important target tissue in ocular treatment. The cellular uptake of starch acetate microparticles and degradation of starch acetate by cultured human RPE-cell line (D407) was examined. Calcein-containing starch acetate microparticles were prepared by a modified water-in-oil-in-water double-emulsion technique. The cellular uptake of the starch acetate microparticles was analysed using flow cytometry and confocal microscopy. Degradation of starch acetate films by the homogenate of lysed RPE cells was determined by gel permeation chromatography. The effect of the microparticles on RPE cell viability was determined by the MTT colorimetric assay. The mean diameter (D50%) of microparticles was 11 μm. During 3-h incubation in RPE-cell culture, 8.1±0.8% of D407 cells took up starch acetate microparticles. Confocal microscopy confirmed the internalisation of microparticles. Incubation of the starch acetate film in the RPE-cell homogenate considerably decreased the molecular weight of starch acetate in the film during 24 h. The viability of cultured RPE cells was at least 82% after 24-h incubation with the microparticles. The present results show that the starch acetate microparticles are taken up by the RPE cells and the polymer can be degraded by the enzymes in these cells. Starch acetate microparticles may be suitable for drug delivery to the RPE.

KW - starch acetate

KW - microparticles

KW - retinal pigment epithelium

KW - cellular uptake

KW - enzymatic degradation

KW - biopolymers

U2 - 10.1016/j.jconrel.2004.05.016

DO - 10.1016/j.jconrel.2004.05.016

M3 - Article

VL - 98

SP - 407

EP - 413

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 3

ER -