Starch acetate microparticles for drug delivery into retinal pigment epithelium: In vitro study

Laura Tuovinen (Corresponding Author), Eija Ruhanen, Tarja Kinnarinen, Seppo Rönkkö, Jukka Pelkonen, Arto Urtti, Soili Peltonen, Kristiina Järvinen

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Starch acetates are novel biodegradable polymers which undergo slower degradation and swelling than native starch. Retinal pigment epithelium (RPE) is an important target tissue in ocular treatment. The cellular uptake of starch acetate microparticles and degradation of starch acetate by cultured human RPE-cell line (D407) was examined. Calcein-containing starch acetate microparticles were prepared by a modified water-in-oil-in-water double-emulsion technique. The cellular uptake of the starch acetate microparticles was analysed using flow cytometry and confocal microscopy. Degradation of starch acetate films by the homogenate of lysed RPE cells was determined by gel permeation chromatography. The effect of the microparticles on RPE cell viability was determined by the MTT colorimetric assay. The mean diameter (D50%) of microparticles was 11 μm. During 3-h incubation in RPE-cell culture, 8.1±0.8% of D407 cells took up starch acetate microparticles. Confocal microscopy confirmed the internalisation of microparticles. Incubation of the starch acetate film in the RPE-cell homogenate considerably decreased the molecular weight of starch acetate in the film during 24 h. The viability of cultured RPE cells was at least 82% after 24-h incubation with the microparticles. The present results show that the starch acetate microparticles are taken up by the RPE cells and the polymer can be degraded by the enzymes in these cells. Starch acetate microparticles may be suitable for drug delivery to the RPE.
Original languageEnglish
Pages (from-to)407-413
JournalJournal of Controlled Release
Volume98
Issue number3
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Keywords

  • starch acetate
  • microparticles
  • retinal pigment epithelium
  • cellular uptake
  • enzymatic degradation
  • biopolymers

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