Stationary-phase acid and heat treatments for improvement of the viability of probiotic lactobacilli and bifidobacteria

Maria Saarela (Corresponding Author), Merja Rantala, Katri Hallamaa, Liisa Nohynek, Ilkka Virkajärvi, Jaana Mättö

Research output: Contribution to journalArticleScientificpeer-review

114 Citations (Scopus)

Abstract

Aims: To investigate whether sublethal treatments of stationary‐phase probiotic cultures enhance their survival during lethal treatments and to adapt these treatments to the fermenter‐scale production of probiotic cultures.

Methods and Results: Conditions for acid and heat pretreatments were screened for three Lactobacillus and two Bifidobacterium strains. Strains were sublethally treated both at laboratory scale and at fermenter scale in a strain‐specific manner and exposed to a subsequent lethal treatment. At laboratory scale viability improvement was detected in each strain. However, improvement was more pronounced in the Lactobacillus than in the Bifidobacterium strains. At fermenter scale three strains were tested: for the two Lactobacillus strains a marked improvement in viability was obtained whereas for the Bifidobacterium strain the improvement was either minor or not detected.

Conclusions: Development of treatments for viability enhancement of probiotic strains is feasible, but strain‐specific optimization is necessary to obtain notable improvements.

Significance and Impact of the Study: Strain‐specific treatments were developed for the viability enhancement of stationary‐phase probiotic cells both at laboratory and fermenter scale. These results can be utilised in the production of probiotic cultures with improved viability.

Original languageEnglish
Pages (from-to)1205 - 1214
Number of pages10
JournalJournal of Applied Microbiology
Volume96
Issue number6
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

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Bifidobacterium
Probiotics
Lactobacillus
Hot Temperature
Acids

Keywords

  • probiotic
  • probiotic bacteria
  • probiotics
  • Bifidobacterium
  • Lactobacillus
  • fermentation
  • microbial viability
  • viability

Cite this

Saarela, Maria ; Rantala, Merja ; Hallamaa, Katri ; Nohynek, Liisa ; Virkajärvi, Ilkka ; Mättö, Jaana. / Stationary-phase acid and heat treatments for improvement of the viability of probiotic lactobacilli and bifidobacteria. In: Journal of Applied Microbiology. 2004 ; Vol. 96, No. 6. pp. 1205 - 1214.
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abstract = "Aims: To investigate whether sublethal treatments of stationary‐phase probiotic cultures enhance their survival during lethal treatments and to adapt these treatments to the fermenter‐scale production of probiotic cultures. Methods and Results: Conditions for acid and heat pretreatments were screened for three Lactobacillus and two Bifidobacterium strains. Strains were sublethally treated both at laboratory scale and at fermenter scale in a strain‐specific manner and exposed to a subsequent lethal treatment. At laboratory scale viability improvement was detected in each strain. However, improvement was more pronounced in the Lactobacillus than in the Bifidobacterium strains. At fermenter scale three strains were tested: for the two Lactobacillus strains a marked improvement in viability was obtained whereas for the Bifidobacterium strain the improvement was either minor or not detected. Conclusions: Development of treatments for viability enhancement of probiotic strains is feasible, but strain‐specific optimization is necessary to obtain notable improvements. Significance and Impact of the Study: Strain‐specific treatments were developed for the viability enhancement of stationary‐phase probiotic cells both at laboratory and fermenter scale. These results can be utilised in the production of probiotic cultures with improved viability.",
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Stationary-phase acid and heat treatments for improvement of the viability of probiotic lactobacilli and bifidobacteria. / Saarela, Maria (Corresponding Author); Rantala, Merja; Hallamaa, Katri; Nohynek, Liisa; Virkajärvi, Ilkka; Mättö, Jaana.

In: Journal of Applied Microbiology, Vol. 96, No. 6, 2004, p. 1205 - 1214.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Stationary-phase acid and heat treatments for improvement of the viability of probiotic lactobacilli and bifidobacteria

AU - Saarela, Maria

AU - Rantala, Merja

AU - Hallamaa, Katri

AU - Nohynek, Liisa

AU - Virkajärvi, Ilkka

AU - Mättö, Jaana

PY - 2004

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N2 - Aims: To investigate whether sublethal treatments of stationary‐phase probiotic cultures enhance their survival during lethal treatments and to adapt these treatments to the fermenter‐scale production of probiotic cultures. Methods and Results: Conditions for acid and heat pretreatments were screened for three Lactobacillus and two Bifidobacterium strains. Strains were sublethally treated both at laboratory scale and at fermenter scale in a strain‐specific manner and exposed to a subsequent lethal treatment. At laboratory scale viability improvement was detected in each strain. However, improvement was more pronounced in the Lactobacillus than in the Bifidobacterium strains. At fermenter scale three strains were tested: for the two Lactobacillus strains a marked improvement in viability was obtained whereas for the Bifidobacterium strain the improvement was either minor or not detected. Conclusions: Development of treatments for viability enhancement of probiotic strains is feasible, but strain‐specific optimization is necessary to obtain notable improvements. Significance and Impact of the Study: Strain‐specific treatments were developed for the viability enhancement of stationary‐phase probiotic cells both at laboratory and fermenter scale. These results can be utilised in the production of probiotic cultures with improved viability.

AB - Aims: To investigate whether sublethal treatments of stationary‐phase probiotic cultures enhance their survival during lethal treatments and to adapt these treatments to the fermenter‐scale production of probiotic cultures. Methods and Results: Conditions for acid and heat pretreatments were screened for three Lactobacillus and two Bifidobacterium strains. Strains were sublethally treated both at laboratory scale and at fermenter scale in a strain‐specific manner and exposed to a subsequent lethal treatment. At laboratory scale viability improvement was detected in each strain. However, improvement was more pronounced in the Lactobacillus than in the Bifidobacterium strains. At fermenter scale three strains were tested: for the two Lactobacillus strains a marked improvement in viability was obtained whereas for the Bifidobacterium strain the improvement was either minor or not detected. Conclusions: Development of treatments for viability enhancement of probiotic strains is feasible, but strain‐specific optimization is necessary to obtain notable improvements. Significance and Impact of the Study: Strain‐specific treatments were developed for the viability enhancement of stationary‐phase probiotic cells both at laboratory and fermenter scale. These results can be utilised in the production of probiotic cultures with improved viability.

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