Stimulation of human embryonic stem cell-derived cardiomyocytes on thin-film microelectrodes

Jouko Viitanen (Corresponding Author), Päivi Heimala, Ari Hokkanen, Kristiina Iljin, Erja Kerkelä, Kai Kolari, Hannu Kattelus

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

We describe successful long‐term stimulation of human embryonic stem cell‐derived cardiomyocyte clusters on thin‐film microelectrode structures in vitro. Interdigitated electrode structures were constructed using plain titanium on glass as the electrode material. Titanium rapidly oxidizes in atmospheric conditions to produce an insulating TiOχ layer with high relative permittivity. Capacitive coupling to the incubation medium and to the cells adherent to the electrodes was still efficient, and the dielectric layer prevented electrolysis, allowing a wider window of possible stimulation amplitudes to be used, relative to conducting surfaces. A common hypothesis suggests that to achieve proper differentiation of electroactive cells from the stem cells electrical stimuli are also needed. Spontaneously beating cardiomyocyte clusters were seeded on the glass‐electrode surfaces, and we successfully altered and resynchronized a clearly different beat interval. The new pace was reliably maintained for extended periods of several tens of minutes.
Original languageEnglish
Pages (from-to)600-603
Number of pages4
JournalBiotechnology Journal
Volume6
Issue number5
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

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Microelectrodes
Cardiac Myocytes
Electrodes
Titanium
Electrolysis
Glass
Cell Differentiation
Stem Cells
Human Embryonic Stem Cells

Keywords

  • Cardiomyocytes
  • Microarrays
  • Microelectrode arrays
  • Stem cells
  • Titanium oxide

Cite this

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title = "Stimulation of human embryonic stem cell-derived cardiomyocytes on thin-film microelectrodes",
abstract = "We describe successful long‐term stimulation of human embryonic stem cell‐derived cardiomyocyte clusters on thin‐film microelectrode structures in vitro. Interdigitated electrode structures were constructed using plain titanium on glass as the electrode material. Titanium rapidly oxidizes in atmospheric conditions to produce an insulating TiOχ layer with high relative permittivity. Capacitive coupling to the incubation medium and to the cells adherent to the electrodes was still efficient, and the dielectric layer prevented electrolysis, allowing a wider window of possible stimulation amplitudes to be used, relative to conducting surfaces. A common hypothesis suggests that to achieve proper differentiation of electroactive cells from the stem cells electrical stimuli are also needed. Spontaneously beating cardiomyocyte clusters were seeded on the glass‐electrode surfaces, and we successfully altered and resynchronized a clearly different beat interval. The new pace was reliably maintained for extended periods of several tens of minutes.",
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Stimulation of human embryonic stem cell-derived cardiomyocytes on thin-film microelectrodes. / Viitanen, Jouko (Corresponding Author); Heimala, Päivi; Hokkanen, Ari; Iljin, Kristiina; Kerkelä, Erja; Kolari, Kai; Kattelus, Hannu.

In: Biotechnology Journal, Vol. 6, No. 5, 2011, p. 600-603.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Stimulation of human embryonic stem cell-derived cardiomyocytes on thin-film microelectrodes

AU - Viitanen, Jouko

AU - Heimala, Päivi

AU - Hokkanen, Ari

AU - Iljin, Kristiina

AU - Kerkelä, Erja

AU - Kolari, Kai

AU - Kattelus, Hannu

PY - 2011

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AB - We describe successful long‐term stimulation of human embryonic stem cell‐derived cardiomyocyte clusters on thin‐film microelectrode structures in vitro. Interdigitated electrode structures were constructed using plain titanium on glass as the electrode material. Titanium rapidly oxidizes in atmospheric conditions to produce an insulating TiOχ layer with high relative permittivity. Capacitive coupling to the incubation medium and to the cells adherent to the electrodes was still efficient, and the dielectric layer prevented electrolysis, allowing a wider window of possible stimulation amplitudes to be used, relative to conducting surfaces. A common hypothesis suggests that to achieve proper differentiation of electroactive cells from the stem cells electrical stimuli are also needed. Spontaneously beating cardiomyocyte clusters were seeded on the glass‐electrode surfaces, and we successfully altered and resynchronized a clearly different beat interval. The new pace was reliably maintained for extended periods of several tens of minutes.

KW - Cardiomyocytes

KW - Microarrays

KW - Microelectrode arrays

KW - Stem cells

KW - Titanium oxide

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JF - Biotechnology Journal

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