Strain and process production of polygalacturonase

Michael Bailey (Corresponding Author), Erkki Pessa

Research output: Contribution to journalArticleScientificpeer-review

91 Citations (Scopus)

Abstract

A process was developed for the production of polygalacturonase (poly-1,4-α-d-galacturonide glycanohydrolase, EC 3.2.1.15) by Aspergillus niger VTT-D-77050 and its mutant VTT-D-86267. High levels of production were obtained in laboratory fermentations on a beet extraction waste medium. The yields were lower in pilot scale production experiments, although the producing strains were stable in the process conditions. The enzyme mixture produced in one pilot fermentation was used successfully for improving the cloud stability of fruit nectars. In addition to polygalacturonase, the preparation also contained appreciable levels of some other important activities.
Original languageEnglish
Pages (from-to)266-271
JournalEnzyme and Microbial Technology
Volume12
Issue number4
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

Fingerprint

Fermentation
Plant Nectar
Beta vulgaris
Aspergillus niger
Fruit
Aspergillus
Fruits
Enzymes
Experiments

Cite this

Bailey, Michael ; Pessa, Erkki. / Strain and process production of polygalacturonase. In: Enzyme and Microbial Technology. 1990 ; Vol. 12, No. 4. pp. 266-271.
@article{1f1b77e5fb5248e1bcf4b6a65182de19,
title = "Strain and process production of polygalacturonase",
abstract = "A process was developed for the production of polygalacturonase (poly-1,4-α-d-galacturonide glycanohydrolase, EC 3.2.1.15) by Aspergillus niger VTT-D-77050 and its mutant VTT-D-86267. High levels of production were obtained in laboratory fermentations on a beet extraction waste medium. The yields were lower in pilot scale production experiments, although the producing strains were stable in the process conditions. The enzyme mixture produced in one pilot fermentation was used successfully for improving the cloud stability of fruit nectars. In addition to polygalacturonase, the preparation also contained appreciable levels of some other important activities.",
author = "Michael Bailey and Erkki Pessa",
year = "1990",
doi = "10.1016/0141-0229(90)90098-B",
language = "English",
volume = "12",
pages = "266--271",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier",
number = "4",

}

Strain and process production of polygalacturonase. / Bailey, Michael (Corresponding Author); Pessa, Erkki.

In: Enzyme and Microbial Technology, Vol. 12, No. 4, 1990, p. 266-271.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Strain and process production of polygalacturonase

AU - Bailey, Michael

AU - Pessa, Erkki

PY - 1990

Y1 - 1990

N2 - A process was developed for the production of polygalacturonase (poly-1,4-α-d-galacturonide glycanohydrolase, EC 3.2.1.15) by Aspergillus niger VTT-D-77050 and its mutant VTT-D-86267. High levels of production were obtained in laboratory fermentations on a beet extraction waste medium. The yields were lower in pilot scale production experiments, although the producing strains were stable in the process conditions. The enzyme mixture produced in one pilot fermentation was used successfully for improving the cloud stability of fruit nectars. In addition to polygalacturonase, the preparation also contained appreciable levels of some other important activities.

AB - A process was developed for the production of polygalacturonase (poly-1,4-α-d-galacturonide glycanohydrolase, EC 3.2.1.15) by Aspergillus niger VTT-D-77050 and its mutant VTT-D-86267. High levels of production were obtained in laboratory fermentations on a beet extraction waste medium. The yields were lower in pilot scale production experiments, although the producing strains were stable in the process conditions. The enzyme mixture produced in one pilot fermentation was used successfully for improving the cloud stability of fruit nectars. In addition to polygalacturonase, the preparation also contained appreciable levels of some other important activities.

U2 - 10.1016/0141-0229(90)90098-B

DO - 10.1016/0141-0229(90)90098-B

M3 - Article

VL - 12

SP - 266

EP - 271

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 4

ER -