Strains degrading polysaccharides produced by bacteria from paper machines

Marjaana Rättö, Annikka Mustranta, Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

Biofilm-degrading enzymes are potential agents for slime control in paper machines. In this work, extracellular polysaccharides were produced by bacteria isolated from paper machines and the isolated polysaccharides were used as substrates for the screening of polysaccharide-degrading microbes. Polysaccharide yields of 1.5–3.5 g/l were obtained by ethanol precipitation from cultures of strains of Klebsiella pneumoniae, Bacillus licheniformis and Pseudomonas fluorescens on sucrose medium. Two K. pneumoniae strains apparently produced an identical heteropolysaccharide containing galacturonic acid. Fructose-containing polysaccharides were the main products of B. licheniformis and P. fluorescens. Bacteria capable of hydrolyzing the fructose-containing polymers (levans) appeared to be relatively common among the strains selected for screening. None of the bacteria or mixed cultures screened were able to utilize the Klebsiella heteropolysaccharides.
Original languageEnglish
Pages (from-to)182-185
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume57
Issue number1-2
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

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Polysaccharides
Bacteria
Pseudomonas fluorescens
Klebsiella pneumoniae
Fructose
Fructans
Klebsiella
Biofilms
Sucrose
Polymers
Ethanol
Enzymes
Bacillus licheniformis

Cite this

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title = "Strains degrading polysaccharides produced by bacteria from paper machines",
abstract = "Biofilm-degrading enzymes are potential agents for slime control in paper machines. In this work, extracellular polysaccharides were produced by bacteria isolated from paper machines and the isolated polysaccharides were used as substrates for the screening of polysaccharide-degrading microbes. Polysaccharide yields of 1.5–3.5 g/l were obtained by ethanol precipitation from cultures of strains of Klebsiella pneumoniae, Bacillus licheniformis and Pseudomonas fluorescens on sucrose medium. Two K. pneumoniae strains apparently produced an identical heteropolysaccharide containing galacturonic acid. Fructose-containing polysaccharides were the main products of B. licheniformis and P. fluorescens. Bacteria capable of hydrolyzing the fructose-containing polymers (levans) appeared to be relatively common among the strains selected for screening. None of the bacteria or mixed cultures screened were able to utilize the Klebsiella heteropolysaccharides.",
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Strains degrading polysaccharides produced by bacteria from paper machines. / Rättö, Marjaana; Mustranta, Annikka; Siika-aho, Matti.

In: Applied Microbiology and Biotechnology, Vol. 57, No. 1-2, 2001, p. 182-185.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Strains degrading polysaccharides produced by bacteria from paper machines

AU - Rättö, Marjaana

AU - Mustranta, Annikka

AU - Siika-aho, Matti

PY - 2001

Y1 - 2001

N2 - Biofilm-degrading enzymes are potential agents for slime control in paper machines. In this work, extracellular polysaccharides were produced by bacteria isolated from paper machines and the isolated polysaccharides were used as substrates for the screening of polysaccharide-degrading microbes. Polysaccharide yields of 1.5–3.5 g/l were obtained by ethanol precipitation from cultures of strains of Klebsiella pneumoniae, Bacillus licheniformis and Pseudomonas fluorescens on sucrose medium. Two K. pneumoniae strains apparently produced an identical heteropolysaccharide containing galacturonic acid. Fructose-containing polysaccharides were the main products of B. licheniformis and P. fluorescens. Bacteria capable of hydrolyzing the fructose-containing polymers (levans) appeared to be relatively common among the strains selected for screening. None of the bacteria or mixed cultures screened were able to utilize the Klebsiella heteropolysaccharides.

AB - Biofilm-degrading enzymes are potential agents for slime control in paper machines. In this work, extracellular polysaccharides were produced by bacteria isolated from paper machines and the isolated polysaccharides were used as substrates for the screening of polysaccharide-degrading microbes. Polysaccharide yields of 1.5–3.5 g/l were obtained by ethanol precipitation from cultures of strains of Klebsiella pneumoniae, Bacillus licheniformis and Pseudomonas fluorescens on sucrose medium. Two K. pneumoniae strains apparently produced an identical heteropolysaccharide containing galacturonic acid. Fructose-containing polysaccharides were the main products of B. licheniformis and P. fluorescens. Bacteria capable of hydrolyzing the fructose-containing polymers (levans) appeared to be relatively common among the strains selected for screening. None of the bacteria or mixed cultures screened were able to utilize the Klebsiella heteropolysaccharides.

U2 - 10.1007/s002530100729

DO - 10.1007/s002530100729

M3 - Article

VL - 57

SP - 182

EP - 185

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

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