Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

Susana Rodríguez Couto; Marjaana Rättö; Alberto Domínguez; Angeles Sanromán

doi:10.1016/S0032-9592(01)00139-X

Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

Susana Rodríguez Couto, Marjaana Rättö, Alberto Domínguez, Angeles Sanromán (Corresponding Author)

VTT Technical Research Centre of Finland

University of Vigo

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

Abstract

The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.

Original language	English
Pages (from-to)	995-999
Journal	Process Biochemistry
Volume	36
Issue number	10
DOIs	https://doi.org/10.1016/S0032-9592(01)00139-X
Publication status	Published - 2001
MoE publication type	A1 Journal article-refereed

Access to Document

10.1016/S0032-9592(01)00139-X

Cite this

@article{0bfc7aed8eb547e3b35a06c78da68b19,

title = "Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors",

abstract = "The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.",

author = "{Rodr{\'i}guez Couto}, Susana and Marjaana R{\"a}tt{\"o} and Alberto Dom{\'i}nguez and Angeles Sanrom{\'a}n",

year = "2001",

doi = "10.1016/S0032-9592(01)00139-X",

language = "English",

volume = "36",

pages = "995--999",

journal = "Process Biochemistry",

issn = "1359-5113",

publisher = "Elsevier",

number = "10",

}

TY - JOUR

T1 - Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

AU - Rodríguez Couto, Susana

AU - Rättö, Marjaana

AU - Domínguez, Alberto

AU - Sanromán, Angeles

PY - 2001

Y1 - 2001

N2 - The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.

AB - The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.

U2 - 10.1016/S0032-9592(01)00139-X

DO - 10.1016/S0032-9592(01)00139-X

M3 - Article

SN - 1359-5113

VL - 36

SP - 995

EP - 999

JO - Process Biochemistry

JF - Process Biochemistry

IS - 10

ER -

Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

Abstract

Access to Document

Fingerprint

Cite this