Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

Susana Rodríguez Couto; Marjaana Rättö; Alberto Domínguez; Angeles Sanromán

doi:10.1016/S0032-9592(01)00139-X

Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

Susana Rodríguez Couto
, Marjaana Rättö
, Alberto Domínguez
, Angeles Sanromán^*

^*Corresponding author for this work

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

14 Citations (Scopus)

Abstract

The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.

Original language	English
Pages (from-to)	995-999
Journal	Process Biochemistry
Volume	36
Issue number	10
DOIs	https://doi.org/10.1016/S0032-9592(01)00139-X
Publication status	Published - 2001
MoE publication type	A1 Journal article-refereed

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title = "Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors",

abstract = "The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05\% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40\% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.",

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doi = "10.1016/S0032-9592(01)00139-X",

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journal = "Process Biochemistry",

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T1 - Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

AU - Rodríguez Couto, Susana

AU - Rättö, Marjaana

AU - Domínguez, Alberto

AU - Sanromán, Angeles

PY - 2001

Y1 - 2001

N2 - The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.

AB - The production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was studied in laboratory-scale bioreactors, operating under semi-solid-state conditions. The bioreactors were filled with inert support (polypropylene sponges) and cultivation was carried out in batch. Veratryl alcohol (2 mM) and Tween 80 (0.05% v/v) were added at the beginning of the fermentation to stimulate the production of ligninolytic enzymes. A modification in the design of a bioreactor, which operates in semi-solid-state conditions, lead to a significant improvement in the ligninolytic enzyme activities obtained. Thus, by modifying the design of the bioreactor employed in the current study, it was possible to increase about tenfold manganese-dependent peroxidase (MnP) activities and around 40% lignin peroxidase (LiP) activities. Moreover, both MnP and LiP profiles were more regular in the modified bioreactor.

U2 - 10.1016/S0032-9592(01)00139-X

DO - 10.1016/S0032-9592(01)00139-X

M3 - Article

SN - 1359-5113

VL - 36

SP - 995

EP - 999

JO - Process Biochemistry

JF - Process Biochemistry

IS - 10

ER -

Strategies for improving ligninolytic enzyme activities in semi-solid-state bioreactors

Abstract

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