Structural and chemical analysis of native and malted barley kernels by polarized Raman spectroscopy (PRS)

Leonardo Galvis, Carlo G. Bertinetto, Ulla Holopainen, Tarja Tamminen, Tapani Vuorinen

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

In this work, barley kernel sections embedded in resin were mapped using polarized Raman spectroscopy (PRS) for evaluation of both chemical and structural changes in endosperm upon malting. A multivariate analysis that includes Band-Target Entropy Minimization (BTEM) was used to resolve the individual spectrum of components present on sections and to reconstruct images based on their relative signal. BTEM-resolved spectra matched with several individual model compound spectra that allowed the identification of protein, starch, non-starch carbohydrates, esterified ferulic acid and embedding media. Raman images also revealed chemical changes in the cell walls within starchy endosperm and inside cells due to enzymatic reactions in malting. The anisotropic response of the C–O–C Raman band associated with the orientation of ordered structures inside starch granules remains despite a notorious shape distortion of large starch granules.
Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalJournal of Cereal Science
Volume62
DOIs
Publication statusPublished - Mar 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Raman spectroscopy
malting
Raman Spectrum Analysis
Hordeum
entropy
starch granules
Structural analysis
chemical analysis
Starch
endosperm
Endosperm
ferulic acid
barley
Entropy
enzymatic reactions
seeds
Chemical analysis
multivariate analysis
resins
cell walls

Keywords

  • AFM
  • Atomic force microscopy
  • BTEM
  • Band-Target Entropy Minimization
  • CV
  • Chemical imaging
  • Coefficient of Variation
  • LLS
  • Linear least-squares fit
  • Malting
  • Multivariate analysis
  • PRS
  • Polarized Raman Spectroscopy
  • Polarized Raman spectroscopy
  • SD
  • Standard Deviation

Cite this

@article{089b4938b16847f88173e306c285b894,
title = "Structural and chemical analysis of native and malted barley kernels by polarized Raman spectroscopy (PRS)",
abstract = "In this work, barley kernel sections embedded in resin were mapped using polarized Raman spectroscopy (PRS) for evaluation of both chemical and structural changes in endosperm upon malting. A multivariate analysis that includes Band-Target Entropy Minimization (BTEM) was used to resolve the individual spectrum of components present on sections and to reconstruct images based on their relative signal. BTEM-resolved spectra matched with several individual model compound spectra that allowed the identification of protein, starch, non-starch carbohydrates, esterified ferulic acid and embedding media. Raman images also revealed chemical changes in the cell walls within starchy endosperm and inside cells due to enzymatic reactions in malting. The anisotropic response of the C–O–C Raman band associated with the orientation of ordered structures inside starch granules remains despite a notorious shape distortion of large starch granules.",
keywords = "AFM, Atomic force microscopy, BTEM, Band-Target Entropy Minimization, CV, Chemical imaging, Coefficient of Variation, LLS, Linear least-squares fit, Malting, Multivariate analysis, PRS, Polarized Raman Spectroscopy, Polarized Raman spectroscopy, SD, Standard Deviation",
author = "Leonardo Galvis and Bertinetto, {Carlo G.} and Ulla Holopainen and Tarja Tamminen and Tapani Vuorinen",
year = "2015",
month = "3",
doi = "10.1016/j.jcs.2014.12.008",
language = "English",
volume = "62",
pages = "73--80",
journal = "Journal of Cereal Science",
issn = "0733-5210",
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}

Structural and chemical analysis of native and malted barley kernels by polarized Raman spectroscopy (PRS). / Galvis, Leonardo; Bertinetto, Carlo G.; Holopainen, Ulla; Tamminen, Tarja; Vuorinen, Tapani.

In: Journal of Cereal Science, Vol. 62, 03.2015, p. 73-80.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Structural and chemical analysis of native and malted barley kernels by polarized Raman spectroscopy (PRS)

AU - Galvis, Leonardo

AU - Bertinetto, Carlo G.

AU - Holopainen, Ulla

AU - Tamminen, Tarja

AU - Vuorinen, Tapani

PY - 2015/3

Y1 - 2015/3

N2 - In this work, barley kernel sections embedded in resin were mapped using polarized Raman spectroscopy (PRS) for evaluation of both chemical and structural changes in endosperm upon malting. A multivariate analysis that includes Band-Target Entropy Minimization (BTEM) was used to resolve the individual spectrum of components present on sections and to reconstruct images based on their relative signal. BTEM-resolved spectra matched with several individual model compound spectra that allowed the identification of protein, starch, non-starch carbohydrates, esterified ferulic acid and embedding media. Raman images also revealed chemical changes in the cell walls within starchy endosperm and inside cells due to enzymatic reactions in malting. The anisotropic response of the C–O–C Raman band associated with the orientation of ordered structures inside starch granules remains despite a notorious shape distortion of large starch granules.

AB - In this work, barley kernel sections embedded in resin were mapped using polarized Raman spectroscopy (PRS) for evaluation of both chemical and structural changes in endosperm upon malting. A multivariate analysis that includes Band-Target Entropy Minimization (BTEM) was used to resolve the individual spectrum of components present on sections and to reconstruct images based on their relative signal. BTEM-resolved spectra matched with several individual model compound spectra that allowed the identification of protein, starch, non-starch carbohydrates, esterified ferulic acid and embedding media. Raman images also revealed chemical changes in the cell walls within starchy endosperm and inside cells due to enzymatic reactions in malting. The anisotropic response of the C–O–C Raman band associated with the orientation of ordered structures inside starch granules remains despite a notorious shape distortion of large starch granules.

KW - AFM

KW - Atomic force microscopy

KW - BTEM

KW - Band-Target Entropy Minimization

KW - CV

KW - Chemical imaging

KW - Coefficient of Variation

KW - LLS

KW - Linear least-squares fit

KW - Malting

KW - Multivariate analysis

KW - PRS

KW - Polarized Raman Spectroscopy

KW - Polarized Raman spectroscopy

KW - SD

KW - Standard Deviation

U2 - 10.1016/j.jcs.2014.12.008

DO - 10.1016/j.jcs.2014.12.008

M3 - Article

VL - 62

SP - 73

EP - 80

JO - Journal of Cereal Science

JF - Journal of Cereal Science

SN - 0733-5210

ER -