Structural and functional characteristics of chimeric avidins physically adsorbed onto functionalized polythiophene thin films

Willem M. Albers (Corresponding Author), Jani M. Pelto, Clément Suspène, Juha A. Määttä, Abderrahim Yassar, Vesa P. Hytönen, Inger M. Vikholm-Lundin, Kirsi Tappura

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    10 Citations (Scopus)

    Abstract

    Stabilized bioreceptor layers are of great importance in the design of novel biosensors. In earlier work, chimeric avidins enabled immobilization of biotinylated antibodies onto gold surfaces with greater stability compared to more conventional avidins (wild-type avidin and streptavidin). In the present study, the applicability of chimeric avidins as a general binding scaffold for biotinylated antibodies on spin-coated functionalized polythiophene thin films has been studied by surface plasmon resonance and atomic force microscopy. Novel chimeric avidins showed remarkably increased binding characteristics compared with other avidins, such as wild-type avidin, streptavidin, and bacterial avidin when merely physically adsorbed onto the polythiophene surface. They gave the highest binding capacities, the highest affinity constant, and the highest stability for biotinylated probe immobilization. Introduction of carboxylic acid groups to polythiophene layer further enhanced the binding level of the avidins. Polythiophene layers functionalized with chimeric avidins thus offered a promising generic platform for biosensor applications.
    Original languageEnglish
    Pages (from-to)4067-4077
    Number of pages10
    JournalACS Applied Materials & Interfaces
    Volume4
    Issue number8
    DOIs
    Publication statusPublished - 2012
    MoE publication typeA1 Journal article-refereed

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    Keywords

    • Chimeric avidin
    • polythiophene
    • C-reactive protein
    • antibody immobilization
    • surface plasmon resonance
    • atomic force microscopy

    Cite this

    Albers, W. M., Pelto, J. M., Suspène, C., Määttä, J. A., Yassar, A., Hytönen, V. P., Vikholm-Lundin, I. M., & Tappura, K. (2012). Structural and functional characteristics of chimeric avidins physically adsorbed onto functionalized polythiophene thin films. ACS Applied Materials & Interfaces, 4(8), 4067-4077. https://doi.org/10.1021/am3008517