Structural elucidation of the EPS of slime producing Brevundimonas vesicularis sp. isolated from a paper machine

Rene Verhoef, Pieter de Waard, Henk Schols, Marjaana Rättö, Matti Siika-aho, Alphons Voragen

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39 Citations (Scopus)

Abstract

The slime forming bacteria Brevundimonas vesicularis sp. was isolated from a paper mill and its EPS was produced on laboratory scale. After production, the exopolysaccharide (EPS) was purified and analysed for its purity and homogeneity, HPSEC revealed one distinct population with a molecular mass of more than 2,000 kDa.
The protein content was around 9 w/w%. The sample was analysed to determine its chemical structure.
The EPS was found to consist of rhamnose, glucose, galacturonic acid and glucuronic acid. Due to the presence of uronic acids the molar ratio between the four sugars found varies from 3:5:2:4 by sugar composition analyses after methanolysis to 1:1:1:1 found by NMR. A repeating unit with a molecular mass of 678 Da was confirmed by MALDI-TOF mass spectrometry after mild acid treatment.
13C and 1H hetero- and homonuclear 2D NMR spectroscopy of the native and partial hydrolysed EPS revealed a

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Original languageEnglish
Pages (from-to)1821-1831
JournalCarbohydrate Research
Volume337
Issue number20
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Keywords

  • Brevundimonas vesicularis
  • EPS (slime)
  • Pulp and paper industry
  • 1H and 13C NMR analyses
  • Structure elucidation

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