Structural elucidation of the EPS of slime producing Brevundimonas vesicularis sp. isolated from a paper machine

Rene Verhoef, Pieter de Waard, Henk Schols, Marjaana Rättö, Matti Siika-aho, Alphons Voragen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The slime forming bacteria Brevundimonas vesicularis sp. was isolated from a paper mill and its EPS was produced on laboratory scale. After production, the exopolysaccharide (EPS) was purified and analysed for its purity and homogeneity, HPSEC revealed one distinct population with a molecular mass of more than 2,000 kDa.
The protein content was around 9 w/w%. The sample was analysed to determine its chemical structure.
The EPS was found to consist of rhamnose, glucose, galacturonic acid and glucuronic acid. Due to the presence of uronic acids the molar ratio between the four sugars found varies from 3:5:2:4 by sugar composition analyses after methanolysis to 1:1:1:1 found by NMR. A repeating unit with a molecular mass of 678 Da was confirmed by MALDI-TOF mass spectrometry after mild acid treatment.
13C and 1H hetero- and homonuclear 2D NMR spectroscopy of the native and partial hydrolysed EPS revealed a

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Original languageEnglish
Pages (from-to)1821-1831
JournalCarbohydrate Research
Volume337
Issue number20
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

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Myxococcales
Uronic Acids
Rhamnose
Glucuronic Acid
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Molecular mass
Sugars
Mass Spectrometry
Magnetic Resonance Spectroscopy
Glucose
Acids
Population
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Bacteria
Proteins
Nuclear magnetic resonance
Chemical analysis
galacturonic acid

Keywords

  • Brevundimonas vesicularis
  • EPS (slime)
  • Pulp and paper industry
  • 1H and 13C NMR analyses
  • Structure elucidation

Cite this

Verhoef, Rene ; de Waard, Pieter ; Schols, Henk ; Rättö, Marjaana ; Siika-aho, Matti ; Voragen, Alphons. / Structural elucidation of the EPS of slime producing Brevundimonas vesicularis sp. isolated from a paper machine. In: Carbohydrate Research. 2002 ; Vol. 337, No. 20. pp. 1821-1831.
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Structural elucidation of the EPS of slime producing Brevundimonas vesicularis sp. isolated from a paper machine. / Verhoef, Rene; de Waard, Pieter; Schols, Henk; Rättö, Marjaana; Siika-aho, Matti; Voragen, Alphons.

In: Carbohydrate Research, Vol. 337, No. 20, 2002, p. 1821-1831.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Structural elucidation of the EPS of slime producing Brevundimonas vesicularis sp. isolated from a paper machine

AU - Verhoef, Rene

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