Abstract
Lignin is part of dietary fiber, but its conversion in
the gastrointestinal tract is not well understood. The
aim this study was to obtain structural information of
brewer's spent grain (BSG) lignin and to understand the
behavior of polymeric part of lignin exposed to fecal
microbiota. The original BSG and different lignin
fractions were characterized by pyrolysis-GC/MS with and
without methylation. Methylation pyrolysis proved that
the ratio between guaiacyl and syringyl units was similar
in all lignin samples, but the ratio between p-coumaric
and ferulic acids varied by the isolation method.
Combined pyrolysis results indicated higher acylation of
?-OH groups in syringyl than guaiacyl lignin units. The
polymeric lignin structure in alkali-soluble fraction
after enzymatic hydrolysis was slightly altered in the in
vitro colon fermentation, while lignin in the insoluble
residue after enzymatic treatments remained intact.
Original language | English |
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Pages (from-to) | 812-820 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 64 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2016 |
MoE publication type | A1 Journal article-refereed |
Keywords
- brewer's spent grain
- colon microbiota
- in vitro fermentation
- lignin
- Py-GC/MS
- TMAH/Py