Structure of brewer's spent grain lignin and its interactions with gut microbiota in vitro

Taina Ohra-aho, Piritta Niemi, Anna-Marja Aura, Marco Orlandi, Kaisa Poutanen, Johanna Buchert, Tarja Tamminen

    Research output: Contribution to journalArticleScientificpeer-review

    19 Citations (Scopus)

    Abstract

    Lignin is part of dietary fiber, but its conversion in the gastrointestinal tract is not well understood. The aim this study was to obtain structural information of brewer's spent grain (BSG) lignin and to understand the behavior of polymeric part of lignin exposed to fecal microbiota. The original BSG and different lignin fractions were characterized by pyrolysis-GC/MS with and without methylation. Methylation pyrolysis proved that the ratio between guaiacyl and syringyl units was similar in all lignin samples, but the ratio between p-coumaric and ferulic acids varied by the isolation method. Combined pyrolysis results indicated higher acylation of ?-OH groups in syringyl than guaiacyl lignin units. The polymeric lignin structure in alkali-soluble fraction after enzymatic hydrolysis was slightly altered in the in vitro colon fermentation, while lignin in the insoluble residue after enzymatic treatments remained intact.
    Original languageEnglish
    Pages (from-to)812-820
    JournalJournal of Agricultural and Food Chemistry
    Volume64
    Issue number4
    DOIs
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed

    Keywords

    • brewer's spent grain
    • colon microbiota
    • in vitro fermentation
    • lignin
    • Py-GC/MS
    • TMAH/Py

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