Structure of brewer's spent grain lignin and its interactions with gut microbiota in vitro

Taina Ohra-aho, Piritta Niemi, Anna-Marja Aura, Marco Orlandi, Kaisa Poutanen, Johanna Buchert, Tarja Tamminen

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11 Citations (Scopus)

Abstract

Lignin is part of dietary fiber, but its conversion in the gastrointestinal tract is not well understood. The aim this study was to obtain structural information of brewer's spent grain (BSG) lignin and to understand the behavior of polymeric part of lignin exposed to fecal microbiota. The original BSG and different lignin fractions were characterized by pyrolysis-GC/MS with and without methylation. Methylation pyrolysis proved that the ratio between guaiacyl and syringyl units was similar in all lignin samples, but the ratio between p-coumaric and ferulic acids varied by the isolation method. Combined pyrolysis results indicated higher acylation of ?-OH groups in syringyl than guaiacyl lignin units. The polymeric lignin structure in alkali-soluble fraction after enzymatic hydrolysis was slightly altered in the in vitro colon fermentation, while lignin in the insoluble residue after enzymatic treatments remained intact.
Original languageEnglish
Pages (from-to)812-820
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number4
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

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Keywords

  • brewer's spent grain
  • colon microbiota
  • in vitro fermentation
  • lignin
  • Py-GC/MS
  • TMAH/Py

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