Structure of brewer's spent grain lignin and its interactions with gut microbiota in vitro

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

Lignin is part of dietary fiber, but its conversion in the gastrointestinal tract is not well understood. The aim this study was to obtain structural information of brewer's spent grain (BSG) lignin and to understand the behavior of polymeric part of lignin exposed to fecal microbiota. The original BSG and different lignin fractions were characterized by pyrolysis-GC/MS with and without methylation. Methylation pyrolysis proved that the ratio between guaiacyl and syringyl units was similar in all lignin samples, but the ratio between p-coumaric and ferulic acids varied by the isolation method. Combined pyrolysis results indicated higher acylation of ?-OH groups in syringyl than guaiacyl lignin units. The polymeric lignin structure in alkali-soluble fraction after enzymatic hydrolysis was slightly altered in the in vitro colon fermentation, while lignin in the insoluble residue after enzymatic treatments remained intact.
Original languageEnglish
Pages (from-to)812-820
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number4
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

spent grains
brewers grains
Lignin
intestinal microorganisms
lignin
pyrolysis
ferulic acid
Pyrolysis
Methylation
methylation
Acylation
acylation
Enzymatic hydrolysis
Gastrointestinal Microbiome
In Vitro Techniques
Microbiota
enzymatic treatment
p-coumaric acid
isolation techniques
Dietary Fiber

Keywords

  • brewer's spent grain
  • colon microbiota
  • in vitro fermentation
  • lignin
  • Py-GC/MS
  • TMAH/Py

Cite this

@article{6057d184bdaa4b238fc9750d4523f277,
title = "Structure of brewer's spent grain lignin and its interactions with gut microbiota in vitro",
abstract = "Lignin is part of dietary fiber, but its conversion in the gastrointestinal tract is not well understood. The aim this study was to obtain structural information of brewer's spent grain (BSG) lignin and to understand the behavior of polymeric part of lignin exposed to fecal microbiota. The original BSG and different lignin fractions were characterized by pyrolysis-GC/MS with and without methylation. Methylation pyrolysis proved that the ratio between guaiacyl and syringyl units was similar in all lignin samples, but the ratio between p-coumaric and ferulic acids varied by the isolation method. Combined pyrolysis results indicated higher acylation of ?-OH groups in syringyl than guaiacyl lignin units. The polymeric lignin structure in alkali-soluble fraction after enzymatic hydrolysis was slightly altered in the in vitro colon fermentation, while lignin in the insoluble residue after enzymatic treatments remained intact.",
keywords = "brewer's spent grain, colon microbiota, in vitro fermentation, lignin, Py-GC/MS, TMAH/Py",
author = "Taina Ohra-aho and Piritta Niemi and Anna-Marja Aura and Marco Orlandi and Kaisa Poutanen and Johanna Buchert and Tarja Tamminen",
year = "2016",
doi = "10.1021/acs.jafc.5b05535",
language = "English",
volume = "64",
pages = "812--820",
journal = "Journal of Agricultural and Food Chemistry",
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publisher = "American Chemical Society ACS",
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Structure of brewer's spent grain lignin and its interactions with gut microbiota in vitro. / Ohra-aho, Taina; Niemi, Piritta; Aura, Anna-Marja; Orlandi, Marco; Poutanen, Kaisa; Buchert, Johanna; Tamminen, Tarja.

In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 4, 2016, p. 812-820.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Structure of brewer's spent grain lignin and its interactions with gut microbiota in vitro

AU - Ohra-aho, Taina

AU - Niemi, Piritta

AU - Aura, Anna-Marja

AU - Orlandi, Marco

AU - Poutanen, Kaisa

AU - Buchert, Johanna

AU - Tamminen, Tarja

PY - 2016

Y1 - 2016

N2 - Lignin is part of dietary fiber, but its conversion in the gastrointestinal tract is not well understood. The aim this study was to obtain structural information of brewer's spent grain (BSG) lignin and to understand the behavior of polymeric part of lignin exposed to fecal microbiota. The original BSG and different lignin fractions were characterized by pyrolysis-GC/MS with and without methylation. Methylation pyrolysis proved that the ratio between guaiacyl and syringyl units was similar in all lignin samples, but the ratio between p-coumaric and ferulic acids varied by the isolation method. Combined pyrolysis results indicated higher acylation of ?-OH groups in syringyl than guaiacyl lignin units. The polymeric lignin structure in alkali-soluble fraction after enzymatic hydrolysis was slightly altered in the in vitro colon fermentation, while lignin in the insoluble residue after enzymatic treatments remained intact.

AB - Lignin is part of dietary fiber, but its conversion in the gastrointestinal tract is not well understood. The aim this study was to obtain structural information of brewer's spent grain (BSG) lignin and to understand the behavior of polymeric part of lignin exposed to fecal microbiota. The original BSG and different lignin fractions were characterized by pyrolysis-GC/MS with and without methylation. Methylation pyrolysis proved that the ratio between guaiacyl and syringyl units was similar in all lignin samples, but the ratio between p-coumaric and ferulic acids varied by the isolation method. Combined pyrolysis results indicated higher acylation of ?-OH groups in syringyl than guaiacyl lignin units. The polymeric lignin structure in alkali-soluble fraction after enzymatic hydrolysis was slightly altered in the in vitro colon fermentation, while lignin in the insoluble residue after enzymatic treatments remained intact.

KW - brewer's spent grain

KW - colon microbiota

KW - in vitro fermentation

KW - lignin

KW - Py-GC/MS

KW - TMAH/Py

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DO - 10.1021/acs.jafc.5b05535

M3 - Article

VL - 64

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JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

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ER -