Studies on growth and cardenolide production of Digitalis lanata tissue cultures

Ulla Tuominen, Leena Toivonen, Veli Kauppinen, Pertti Markkanen, Lars Björk

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides.
The possibility of using a statistical (Box–Wilson) experimental design to study the effects of four different variables on growth, differentiation, and cardenolide production of D. lanata tissue cultures are investigated. The results of the analyses were processed by linear regression analysis.
Mathematical models explaining the effects of the variables were developed. The concentration of maltose and the NO3−NH4+ ratio were found to be significant variables for both growth and cardenolide production.
The size of the inoculum was also important.
Original languageEnglish
Pages (from-to)558-562
JournalBiotechnology and Bioengineering
Volume33
Issue number5
DOIs
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed

Fingerprint

Cardenolides
Tissue culture
Digitalis
Maltose
Growth
Cell culture
Linear regression
Regression analysis
Design of experiments
Mathematical models
Linear Models
Suspensions
Research Design
Theoretical Models
Embryonic Structures
Cell Culture Techniques
Regression Analysis

Cite this

Tuominen, Ulla ; Toivonen, Leena ; Kauppinen, Veli ; Markkanen, Pertti ; Björk, Lars. / Studies on growth and cardenolide production of Digitalis lanata tissue cultures. In: Biotechnology and Bioengineering. 1989 ; Vol. 33, No. 5. pp. 558-562.
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abstract = "Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides. The possibility of using a statistical (Box–Wilson) experimental design to study the effects of four different variables on growth, differentiation, and cardenolide production of D. lanata tissue cultures are investigated. The results of the analyses were processed by linear regression analysis. Mathematical models explaining the effects of the variables were developed. The concentration of maltose and the NO3−NH4+ ratio were found to be significant variables for both growth and cardenolide production. The size of the inoculum was also important.",
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Tuominen, U, Toivonen, L, Kauppinen, V, Markkanen, P & Björk, L 1989, 'Studies on growth and cardenolide production of Digitalis lanata tissue cultures', Biotechnology and Bioengineering, vol. 33, no. 5, pp. 558-562. https://doi.org/10.1002/bit.260330507

Studies on growth and cardenolide production of Digitalis lanata tissue cultures. / Tuominen, Ulla; Toivonen, Leena; Kauppinen, Veli; Markkanen, Pertti; Björk, Lars.

In: Biotechnology and Bioengineering, Vol. 33, No. 5, 1989, p. 558-562.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Tuominen, Ulla

AU - Toivonen, Leena

AU - Kauppinen, Veli

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AU - Björk, Lars

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AB - Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides. The possibility of using a statistical (Box–Wilson) experimental design to study the effects of four different variables on growth, differentiation, and cardenolide production of D. lanata tissue cultures are investigated. The results of the analyses were processed by linear regression analysis. Mathematical models explaining the effects of the variables were developed. The concentration of maltose and the NO3−NH4+ ratio were found to be significant variables for both growth and cardenolide production. The size of the inoculum was also important.

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