Abstract
| Original language | English |
|---|---|
| Title of host publication | Proceedings MME 2012 |
| Subtitle of host publication | Micromechanics and Microsystems Europe Workshop 2012 |
| Number of pages | 4 |
| Publication status | Published - 2012 |
| MoE publication type | A4 Article in a conference publication |
| Event | 23rd Micromechanics and Microsystems Europe Workshop, MME 2012 - Ilmenau, Germany Duration: 9 Sep 2012 → 12 Sep 2012 |
Workshop
| Workshop | 23rd Micromechanics and Microsystems Europe Workshop, MME 2012 |
|---|---|
| Abbreviated title | MME 2012 |
| Country | Germany |
| City | Ilmenau |
| Period | 9/09/12 → 12/09/12 |
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Keywords
- Microfluidic
- silicon
- PDMS
- E. coli
- ciprofloxacin
- antibiotic resistance
Cite this
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Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. / Kolari, Kai; Mojzita, Dominik; Stuns, Ingmar; Penttilä, Merja; Hjort, Klas.
Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012. 2012.Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review
TY - GEN
T1 - Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient
AU - Kolari, Kai
AU - Mojzita, Dominik
AU - Stuns, Ingmar
AU - Penttilä, Merja
AU - Hjort, Klas
N1 - CA2: TK610 CA2: TK402 CA2: TK400 SDA: MEL
PY - 2012
Y1 - 2012
N2 - In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.
AB - In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.
KW - Microfluidic
KW - silicon
KW - PDMS
KW - E. coli
KW - ciprofloxacin
KW - antibiotic resistance
M3 - Conference article in proceedings
BT - Proceedings MME 2012
ER -