Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient

Kai Kolari, Dominik Mojzita, Ingmar Stuns, Merja Penttilä, Klas Hjort

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.
Original languageEnglish
Title of host publicationProceedings MME 2012
Subtitle of host publicationMicromechanics and Microsystems Europe Workshop 2012
Number of pages4
Publication statusPublished - 2012
MoE publication typeA4 Article in a conference publication
Event23rd Micromechanics and Microsystems Europe Workshop, MME 2012 - Ilmenau, Germany
Duration: 9 Sep 201212 Sep 2012

Workshop

Workshop23rd Micromechanics and Microsystems Europe Workshop, MME 2012
Abbreviated titleMME 2012
CountryGermany
CityIlmenau
Period9/09/1212/09/12

Fingerprint

ciprofloxacin
population dynamics
antibiotics
Escherichia coli
microhabitats
silicon
green fluorescent protein
cell viability
biomass production
viability
cells
liquids
monitoring

Keywords

  • Microfluidic
  • silicon
  • PDMS
  • E. coli
  • ciprofloxacin
  • antibiotic resistance

Cite this

Kolari, K., Mojzita, D., Stuns, I., Penttilä, M., & Hjort, K. (2012). Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. In Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012
Kolari, Kai ; Mojzita, Dominik ; Stuns, Ingmar ; Penttilä, Merja ; Hjort, Klas. / Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012. 2012.
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Kolari, K, Mojzita, D, Stuns, I, Penttilä, M & Hjort, K 2012, Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. in Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012. 23rd Micromechanics and Microsystems Europe Workshop, MME 2012, Ilmenau, Germany, 9/09/12.

Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. / Kolari, Kai; Mojzita, Dominik; Stuns, Ingmar; Penttilä, Merja; Hjort, Klas.

Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012. 2012.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

TY - GEN

T1 - Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient

AU - Kolari, Kai

AU - Mojzita, Dominik

AU - Stuns, Ingmar

AU - Penttilä, Merja

AU - Hjort, Klas

N1 - CA2: TK610 CA2: TK402 CA2: TK400 SDA: MEL

PY - 2012

Y1 - 2012

N2 - In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.

AB - In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.

KW - Microfluidic

KW - silicon

KW - PDMS

KW - E. coli

KW - ciprofloxacin

KW - antibiotic resistance

M3 - Conference article in proceedings

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Kolari K, Mojzita D, Stuns I, Penttilä M, Hjort K. Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. In Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012. 2012