Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient

Kai Kolari; Dominik Mojzita; Ingmar Stuns; Merja Penttilä; Klas Hjort

Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient

Kai Kolari, Dominik Mojzita, Ingmar Stuns, Merja Penttilä, Klas Hjort

VTT Technical Research Centre of Finland

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.

Original language	English
Title of host publication	Proceedings MME 2012
Subtitle of host publication	Micromechanics and Microsystems Europe Workshop 2012
Number of pages	4
Publication status	Published - 2012
MoE publication type	A4 Article in a conference publication
Event	23rd Micromechanics and Microsystems Europe Workshop, MME 2012 - Ilmenau, Germany Duration: 9 Sep 2012 → 12 Sep 2012

Workshop

Workshop	23rd Micromechanics and Microsystems Europe Workshop, MME 2012
Abbreviated title	MME 2012
Country/Territory	Germany
City	Ilmenau
Period	9/09/12 → 12/09/12

Keywords

Microfluidic
silicon
PDMS
E. coli
ciprofloxacin
antibiotic resistance

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https://www.tu-ilmenau.de/fileadmin/media/mme2012/Proceedings/Session_D/D11_1635_MME2012_120615.pdf

Cite this

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title = "Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient",

abstract = "In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell{\textquoteright}s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied. ",

keywords = "Microfluidic, silicon, PDMS, E. coli, ciprofloxacin, antibiotic resistance",

author = "Kai Kolari and Dominik Mojzita and Ingmar Stuns and Merja Penttil{\"a} and Klas Hjort",

note = "CA2: TK610 CA2: TK402 CA2: TK400 SDA: MEL; 23rd Micromechanics and Microsystems Europe Workshop, MME 2012, MME 2012 ; Conference date: 09-09-2012 Through 12-09-2012",

year = "2012",

language = "English",

booktitle = "Proceedings MME 2012",

}

Kolari, K, Mojzita, D , Stuns, I , Penttilä, M & Hjort, K 2012, Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. in Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012. 23rd Micromechanics and Microsystems Europe Workshop, MME 2012, Ilmenau, Thuringia, Germany, 9/09/12. <https://www.tu-ilmenau.de/fileadmin/media/mme2012/Proceedings/Session_D/D11_1635_MME2012_120615.pdf>

Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient. / Kolari, Kai; Mojzita, Dominik ; Stuns, Ingmar ; Penttilä, Merja; Hjort, Klas.

Proceedings MME 2012: Micromechanics and Microsystems Europe Workshop 2012. 2012.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient

AU - Kolari, Kai

AU - Mojzita, Dominik

AU - Stuns, Ingmar

AU - Penttilä, Merja

AU - Hjort, Klas

N1 - CA2: TK610 CA2: TK402 CA2: TK400 SDA: MEL

PY - 2012

Y1 - 2012

N2 - In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.

AB - In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.

KW - Microfluidic

KW - silicon

KW - PDMS

KW - E. coli

KW - ciprofloxacin

KW - antibiotic resistance

M3 - Conference article in proceedings

BT - Proceedings MME 2012

T2 - 23rd Micromechanics and Microsystems Europe Workshop, MME 2012

Y2 - 9 September 2012 through 12 September 2012

ER -

Study of population dynamics of E. Coli in a microfluidic landscape incorporating a toxic gradient

Abstract

Workshop

Keywords

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