Abstract
In this study we fabricated a microenvironment in silicon for E. coli cultivation and studied bacterial movement, morphology, biomass accumulation and development of resistance towards ciprofloxacin antibiotic (CIPRO). In the interconnected microhabitats with a CIPRO gradient as well as in the classical liquid cultures with CIPRO, the cells rapidly changed their morphology, lost viability and finally also motility. The emergence of mild resistance toward CIPRO was observed in the microenvironment. In addition we found that green fluorescent protein (GFP), initially intended for monitoring the cell’s viability, is not suitable for such purpose in our system due to its substantial stability. With the microhabitat, dynamics of small populations or at the single cell level could possibly be studied.
Original language | English |
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Title of host publication | Proceedings MME 2012 |
Subtitle of host publication | Micromechanics and Microsystems Europe Workshop 2012 |
Number of pages | 4 |
Publication status | Published - 2012 |
MoE publication type | A4 Article in a conference publication |
Event | 23rd Micromechanics and Microsystems Europe Workshop, MME 2012 - Ilmenau, Germany Duration: 9 Sep 2012 → 12 Sep 2012 |
Workshop
Workshop | 23rd Micromechanics and Microsystems Europe Workshop, MME 2012 |
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Abbreviated title | MME 2012 |
Country/Territory | Germany |
City | Ilmenau |
Period | 9/09/12 → 12/09/12 |
Keywords
- Microfluidic
- silicon
- PDMS
- E. coli
- ciprofloxacin
- antibiotic resistance