Suberin of potato (Solanum tuberosum var. Nikola): Comparison of the effect of cutinase CcCut1 with chemical depolymerization

Riikka Järvinen, Armando J D Silvestre, Ulla Holopainen, Mika Kaimainen, Antti Nyyssölä, Ana M Gil, Carlos Pascoal Neto, Pekka Lehtinen, Johanna Buchert, Heikki Kallio

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

Chemical and enzymatic depolymerizations of suberin isolated from potato peel (Solanum tuberosum var. Nikola) were performed under various conditions. Enzymatic hydrolysis with cutinase CcCut1 and chemical methanolysis with NaOMe of suberin yielded monomeric fragments, which were identified as TMS derivatives with GC-MS and GC-FID. The solid, hydrolysis-resistant residues were analyzed with solid state 13C CPMAS NMR, FT-IR, and microscopic methods. Methanolysis released more CHCl3-soluble material than the cutinase treatment when determined gravimetrically. Interestingly, cutinase-catalyzed hydrolysis produced higher proportions of aliphatic monomers than hydrolysis with the NaOMe procedure when analyzed by GC in the form of TMS derivatives. Monomers released by the two methods were mainly α,ω-dioic acids and ω-hydroxy acids, but the ratios of the detected monomers were different, at 40.0 and 32.7% for methanolysis and 64.6 and 8.2% for cutinase, respectively. Thus, cutinase CcCut1 showed higher activity toward ester bonds of α,ω-dioic acids than toward the bonds of ω-hydroxy acids. The most abundant monomeric compounds were octadec-9-ene-1,18-dioic acid and 18-hydroxyoctadec-9-enoic acid, which accounted for ca. 37 and 28% of all monomers, respectively. The results of the analyses of the chemical and enzymatic hydrolysis products were supported by the spectroscopic analyses with FT-IR and CPMAS 13C NMR together with the analysis of the microstructures of the hydrolysis residues by light and confocal microscopy.
Original languageEnglish
Pages (from-to)9016-9027
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number19
DOIs
Publication statusPublished - Oct 2009
MoE publication typeA1 Journal article-refereed

Keywords

  • Carboxylic Ester Hydrolases
  • Carboxylic Ester Hydrolases: metabolism
  • Fatty Acids
  • Fatty Acids: metabolism
  • Fourier Transform Infrared
  • Hydrolysis
  • Lipids
  • Lipids: chemistry
  • Lipids: isolation & purification
  • Magnetic Resonance Spectroscopy
  • Membrane Lipids
  • Membrane Lipids: metabolism
  • Methanol
  • Methanol: chemistry
  • Plant Tubers
  • Plant Tubers: chemistry
  • Polymers
  • Polymers: chemistry
  • Polymers: metabolism
  • Solanum tuberosum
  • Solanum tuberosum: chemistry
  • Spectroscopy
  • Substrate Specificity

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