Substrate specificities of Penicillium simplicissimum alfa-galactosidases

Elina Luonteri (Corresponding Author), Maija Tenkanen, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

41 Citations (Scopus)

Abstract

The substrate specificities of three Penicillium simplicissimum α-galactosidases, AGLI, AGLII, and AGLIII, were determined by using various isolated galactose-containing oligosaccharides and polymeric galacto(gluco)mannans.
AGLI released galactose from melibiose and raffinose-family oligosaccharides but the amount of galactose released was decreased from 96% to 35% by the increasing chain length of the substrate from raffinose to verbascose. It was able to release galactose linked to the nonreducing end and less efficiently to the internal residues of the galactomanno-oligomers.
AGLI was able to hydrolyze 60–92% of galactose from polymeric galacto(gluco)mannans alone but its action was facilitated by mannanase and β-mannosidase. In addition, it was able to release about 10% of the galactose from softwood kraft pulp alone and about 22% in combination with mannanase. AGLII was highly specific toward small galactose-containing oligosaccharides in which the galactose is linked to the nonreducing end of the substrate. It released 90–100% of galactose present in melibiose, raffinose, stachyose, and verbascose; however, it was able to degrade polymeric substrates only in combination with mannanase and β-mannosidase.
AGLIII had only low activity toward the oligomeric substrates tested. It was able to release some galactose from the polymeric galacto(gluco)mannans alone, but its action was clearly enhanced by the backbone degrading enzymes.

Original languageEnglish
Pages (from-to)192-198
JournalEnzyme and Microbial Technology
Volume22
Issue number3
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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alpha-Galactosidase
Penicillium
Substrate Specificity
Galactose
Oligosaccharides
Substrates
Raffinose
Mannans
Mannosidases
Melibiose
Kraft pulp
Softwoods
Chain length
Oligomers
Enzymes
Galactosidases

Cite this

Luonteri, Elina ; Tenkanen, Maija ; Viikari, Liisa. / Substrate specificities of Penicillium simplicissimum alfa-galactosidases. In: Enzyme and Microbial Technology. 1998 ; Vol. 22, No. 3. pp. 192-198.
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abstract = "The substrate specificities of three Penicillium simplicissimum α-galactosidases, AGLI, AGLII, and AGLIII, were determined by using various isolated galactose-containing oligosaccharides and polymeric galacto(gluco)mannans. AGLI released galactose from melibiose and raffinose-family oligosaccharides but the amount of galactose released was decreased from 96{\%} to 35{\%} by the increasing chain length of the substrate from raffinose to verbascose. It was able to release galactose linked to the nonreducing end and less efficiently to the internal residues of the galactomanno-oligomers. AGLI was able to hydrolyze 60–92{\%} of galactose from polymeric galacto(gluco)mannans alone but its action was facilitated by mannanase and β-mannosidase. In addition, it was able to release about 10{\%} of the galactose from softwood kraft pulp alone and about 22{\%} in combination with mannanase. AGLII was highly specific toward small galactose-containing oligosaccharides in which the galactose is linked to the nonreducing end of the substrate. It released 90–100{\%} of galactose present in melibiose, raffinose, stachyose, and verbascose; however, it was able to degrade polymeric substrates only in combination with mannanase and β-mannosidase. AGLIII had only low activity toward the oligomeric substrates tested. It was able to release some galactose from the polymeric galacto(gluco)mannans alone, but its action was clearly enhanced by the backbone degrading enzymes.",
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Substrate specificities of Penicillium simplicissimum alfa-galactosidases. / Luonteri, Elina (Corresponding Author); Tenkanen, Maija; Viikari, Liisa.

In: Enzyme and Microbial Technology, Vol. 22, No. 3, 1998, p. 192-198.

Research output: Contribution to journalArticleScientificpeer-review

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AB - The substrate specificities of three Penicillium simplicissimum α-galactosidases, AGLI, AGLII, and AGLIII, were determined by using various isolated galactose-containing oligosaccharides and polymeric galacto(gluco)mannans. AGLI released galactose from melibiose and raffinose-family oligosaccharides but the amount of galactose released was decreased from 96% to 35% by the increasing chain length of the substrate from raffinose to verbascose. It was able to release galactose linked to the nonreducing end and less efficiently to the internal residues of the galactomanno-oligomers. AGLI was able to hydrolyze 60–92% of galactose from polymeric galacto(gluco)mannans alone but its action was facilitated by mannanase and β-mannosidase. In addition, it was able to release about 10% of the galactose from softwood kraft pulp alone and about 22% in combination with mannanase. AGLII was highly specific toward small galactose-containing oligosaccharides in which the galactose is linked to the nonreducing end of the substrate. It released 90–100% of galactose present in melibiose, raffinose, stachyose, and verbascose; however, it was able to degrade polymeric substrates only in combination with mannanase and β-mannosidase. AGLIII had only low activity toward the oligomeric substrates tested. It was able to release some galactose from the polymeric galacto(gluco)mannans alone, but its action was clearly enhanced by the backbone degrading enzymes.

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