Suitability of a batch in vitro fermentation model using human faecal microbiota for prediction of conversion of flaxseed lignans to enterolactone with reference to an in vivo rat model

Anna-Marja Aura (Corresponding Author), Seija Oikarinen, Marja Mutanen, Satu-Maarit Heinonen, Herman Adlercreutz, Hannele Virtanen, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

39 Citations (Scopus)

Abstract

Background In vitro fermentation models have been used widely for studies of shortchain fatty acid (SCFA) formation from carbohydrates, whereas the suitability of these methods for enterolactone (ENL) formation has received less attention. Aim The aim was to study the suitability of an in vitro fermentation model for prediction of bioconversion of lignans to ENL, to compare the approach with that of an in vivo rat model and to study the SCFA formation in both models. Methods Predigested samples of rye bran (R), flaxseed meal (F) alone, or in combination with rye bran (R&F) and a faecal control were incubated in an in vitro fermentation model using human faecal microbiota. In the in vivo experiment rats consumed a non–fibre control diet (C) or diets supplemented either with rye bran (R), flaxseed meal (F) alone, or with their combination (R&F) for four weeks. Enterodiol (END), ENL and SCFA concentrations were measured from in vitro faecal fermentation samples and from the intestinal contents of rats. Plasma ENL concentrations from rats were also measured. Results The highest ENL production was found in vitro with the F supplement (areas under curve: 740 ± 4, 7500 ± 400, 2600 ± 500 and 1520 ± 70 nmol · h for the R, F, R&F supplements and faecal control, respectively). In vivo, the concentration of ENL in caecal digesta from flaxseed meal was significantly (P < 0.05) enhanced by the presence of rye bran (medians 261, 407 and 24 nmol/g in the F, R&F and C groups, respectively). No correlation was found between the models regarding ENL production, possibly due to different responses to the presence of rye bran matrix, differences in microbiota or application of a batch in the in vitro fermentation model. Rye bran supplementation enhanced butyrate production both in vitro and in vivo. Conclusion In vitro fermentation and the in vivo rat models responded differently to the presence of rye bran and no correlation with regard to the ENL formation from flaxseed lignans was observed.
Original languageEnglish
Pages (from-to)45 - 51
JournalEuropean Journal of Nutrition
Volume45
Issue number1
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

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Flax
Lignans
Microbiota
Fermentation
Meals
Fatty Acids
Diet
Gastrointestinal Contents
In Vitro Techniques
2,3-bis(3'-hydroxybenzyl)butyrolactone
Butyrates
Secale
Area Under Curve
Carbohydrates

Keywords

  • lignans
  • nondigestible carbohydrates
  • in vitro faecal fermentation
  • in vivo rat model

Cite this

@article{1fd574e6c5674e4d9974b6a4162b97d7,
title = "Suitability of a batch in vitro fermentation model using human faecal microbiota for prediction of conversion of flaxseed lignans to enterolactone with reference to an in vivo rat model",
abstract = "Background In vitro fermentation models have been used widely for studies of shortchain fatty acid (SCFA) formation from carbohydrates, whereas the suitability of these methods for enterolactone (ENL) formation has received less attention. Aim The aim was to study the suitability of an in vitro fermentation model for prediction of bioconversion of lignans to ENL, to compare the approach with that of an in vivo rat model and to study the SCFA formation in both models. Methods Predigested samples of rye bran (R), flaxseed meal (F) alone, or in combination with rye bran (R&F) and a faecal control were incubated in an in vitro fermentation model using human faecal microbiota. In the in vivo experiment rats consumed a non–fibre control diet (C) or diets supplemented either with rye bran (R), flaxseed meal (F) alone, or with their combination (R&F) for four weeks. Enterodiol (END), ENL and SCFA concentrations were measured from in vitro faecal fermentation samples and from the intestinal contents of rats. Plasma ENL concentrations from rats were also measured. Results The highest ENL production was found in vitro with the F supplement (areas under curve: 740 ± 4, 7500 ± 400, 2600 ± 500 and 1520 ± 70 nmol · h for the R, F, R&F supplements and faecal control, respectively). In vivo, the concentration of ENL in caecal digesta from flaxseed meal was significantly (P < 0.05) enhanced by the presence of rye bran (medians 261, 407 and 24 nmol/g in the F, R&F and C groups, respectively). No correlation was found between the models regarding ENL production, possibly due to different responses to the presence of rye bran matrix, differences in microbiota or application of a batch in the in vitro fermentation model. Rye bran supplementation enhanced butyrate production both in vitro and in vivo. Conclusion In vitro fermentation and the in vivo rat models responded differently to the presence of rye bran and no correlation with regard to the ENL formation from flaxseed lignans was observed.",
keywords = "lignans, nondigestible carbohydrates, in vitro faecal fermentation, in vivo rat model",
author = "Anna-Marja Aura and Seija Oikarinen and Marja Mutanen and Satu-Maarit Heinonen and Herman Adlercreutz and Hannele Virtanen and Kaisa Poutanen",
year = "2006",
doi = "10.1007/s00394-005-0561-z",
language = "English",
volume = "45",
pages = "45 -- 51",
journal = "European Journal of Nutrition",
issn = "1436-6207",
publisher = "Elsevier",
number = "1",

}

Suitability of a batch in vitro fermentation model using human faecal microbiota for prediction of conversion of flaxseed lignans to enterolactone with reference to an in vivo rat model. / Aura, Anna-Marja (Corresponding Author); Oikarinen, Seija; Mutanen, Marja; Heinonen, Satu-Maarit; Adlercreutz, Herman; Virtanen, Hannele; Poutanen, Kaisa.

In: European Journal of Nutrition, Vol. 45, No. 1, 2006, p. 45 - 51.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Suitability of a batch in vitro fermentation model using human faecal microbiota for prediction of conversion of flaxseed lignans to enterolactone with reference to an in vivo rat model

AU - Aura, Anna-Marja

AU - Oikarinen, Seija

AU - Mutanen, Marja

AU - Heinonen, Satu-Maarit

AU - Adlercreutz, Herman

AU - Virtanen, Hannele

AU - Poutanen, Kaisa

PY - 2006

Y1 - 2006

N2 - Background In vitro fermentation models have been used widely for studies of shortchain fatty acid (SCFA) formation from carbohydrates, whereas the suitability of these methods for enterolactone (ENL) formation has received less attention. Aim The aim was to study the suitability of an in vitro fermentation model for prediction of bioconversion of lignans to ENL, to compare the approach with that of an in vivo rat model and to study the SCFA formation in both models. Methods Predigested samples of rye bran (R), flaxseed meal (F) alone, or in combination with rye bran (R&F) and a faecal control were incubated in an in vitro fermentation model using human faecal microbiota. In the in vivo experiment rats consumed a non–fibre control diet (C) or diets supplemented either with rye bran (R), flaxseed meal (F) alone, or with their combination (R&F) for four weeks. Enterodiol (END), ENL and SCFA concentrations were measured from in vitro faecal fermentation samples and from the intestinal contents of rats. Plasma ENL concentrations from rats were also measured. Results The highest ENL production was found in vitro with the F supplement (areas under curve: 740 ± 4, 7500 ± 400, 2600 ± 500 and 1520 ± 70 nmol · h for the R, F, R&F supplements and faecal control, respectively). In vivo, the concentration of ENL in caecal digesta from flaxseed meal was significantly (P < 0.05) enhanced by the presence of rye bran (medians 261, 407 and 24 nmol/g in the F, R&F and C groups, respectively). No correlation was found between the models regarding ENL production, possibly due to different responses to the presence of rye bran matrix, differences in microbiota or application of a batch in the in vitro fermentation model. Rye bran supplementation enhanced butyrate production both in vitro and in vivo. Conclusion In vitro fermentation and the in vivo rat models responded differently to the presence of rye bran and no correlation with regard to the ENL formation from flaxseed lignans was observed.

AB - Background In vitro fermentation models have been used widely for studies of shortchain fatty acid (SCFA) formation from carbohydrates, whereas the suitability of these methods for enterolactone (ENL) formation has received less attention. Aim The aim was to study the suitability of an in vitro fermentation model for prediction of bioconversion of lignans to ENL, to compare the approach with that of an in vivo rat model and to study the SCFA formation in both models. Methods Predigested samples of rye bran (R), flaxseed meal (F) alone, or in combination with rye bran (R&F) and a faecal control were incubated in an in vitro fermentation model using human faecal microbiota. In the in vivo experiment rats consumed a non–fibre control diet (C) or diets supplemented either with rye bran (R), flaxseed meal (F) alone, or with their combination (R&F) for four weeks. Enterodiol (END), ENL and SCFA concentrations were measured from in vitro faecal fermentation samples and from the intestinal contents of rats. Plasma ENL concentrations from rats were also measured. Results The highest ENL production was found in vitro with the F supplement (areas under curve: 740 ± 4, 7500 ± 400, 2600 ± 500 and 1520 ± 70 nmol · h for the R, F, R&F supplements and faecal control, respectively). In vivo, the concentration of ENL in caecal digesta from flaxseed meal was significantly (P < 0.05) enhanced by the presence of rye bran (medians 261, 407 and 24 nmol/g in the F, R&F and C groups, respectively). No correlation was found between the models regarding ENL production, possibly due to different responses to the presence of rye bran matrix, differences in microbiota or application of a batch in the in vitro fermentation model. Rye bran supplementation enhanced butyrate production both in vitro and in vivo. Conclusion In vitro fermentation and the in vivo rat models responded differently to the presence of rye bran and no correlation with regard to the ENL formation from flaxseed lignans was observed.

KW - lignans

KW - nondigestible carbohydrates

KW - in vitro faecal fermentation

KW - in vivo rat model

U2 - 10.1007/s00394-005-0561-z

DO - 10.1007/s00394-005-0561-z

M3 - Article

VL - 45

SP - 45

EP - 51

JO - European Journal of Nutrition

JF - European Journal of Nutrition

SN - 1436-6207

IS - 1

ER -