Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products

Johanna Maukonen; Hanna-Leena Alakomi; Liisa Nohynek; Katri Hallamaa; Sanna Leppämäki; Jaana Mättö; Maria Saarela

doi:10.1016/j.foodres.2005.05.006

Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products

Johanna Maukonen (Corresponding Author), Hanna-Leena Alakomi, Liisa Nohynek, Katri Hallamaa, Sanna Leppämäki, Jaana Mättö, Maria Saarela

Research output: Contribution to journal › Article › Scientific › peer-review

53 Citations (Scopus)

Abstract

The suitability of the fluorescent techniques for the microbiological quality assessment of probiotic non-dairy drinks and probiotic pharmaceutical products was studied. The method optimization was first performed with pure cultures of probiotic strains detected in commercial products (representing Lactobacillus and Bifidobacterium species). Altogether seven different fluorescent viability stains combined with epifluorescence microscopy and flow cytometry were tested. The most applicable stains ChemChrome and LIVE/DEAD BacLight Viability Kit were further used for the viability studies of the commercial probiotic products. The studied products proved to be of good quality. All products contained the probiotic bacterium indicated on the label and the levels of living bacteria were reasonable per dose (108–1010 cells for suggested daily dose of pharmaceutical products and 10⁸–10⁹ cells per 100 ml of drink). The results obtained with fluorescent stains were mostly in agreement with results obtained with culture, rendering fluorescent techniques applicable alternatives for rapid viability assessment of probiotic products. Especially the newly developed fluorometry assay with BacLight proved to be fast and accurate.

Original language	English
Pages (from-to)	22-32
Number of pages	11
Journal	Food Research International
Volume	39
Issue number	1
DOIs	https://doi.org/10.1016/j.foodres.2005.05.006
Publication status	Published - 2006
MoE publication type	A1 Journal article-refereed

Keywords

Probiotic product
Lactobacillus
Bifidobacterium
Epifluorescence microscopy
Flow cytometry
Fluorometry assay

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10.1016/j.foodres.2005.05.006

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title = "Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products",

abstract = "The suitability of the fluorescent techniques for the microbiological quality assessment of probiotic non-dairy drinks and probiotic pharmaceutical products was studied. The method optimization was first performed with pure cultures of probiotic strains detected in commercial products (representing Lactobacillus and Bifidobacterium species). Altogether seven different fluorescent viability stains combined with epifluorescence microscopy and flow cytometry were tested. The most applicable stains ChemChrome and LIVE/DEAD BacLight Viability Kit were further used for the viability studies of the commercial probiotic products. The studied products proved to be of good quality. All products contained the probiotic bacterium indicated on the label and the levels of living bacteria were reasonable per dose (108–1010 cells for suggested daily dose of pharmaceutical products and 108–109 cells per 100 ml of drink). The results obtained with fluorescent stains were mostly in agreement with results obtained with culture, rendering fluorescent techniques applicable alternatives for rapid viability assessment of probiotic products. Especially the newly developed fluorometry assay with BacLight proved to be fast and accurate.",

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author = "Johanna Maukonen and Hanna-Leena Alakomi and Liisa Nohynek and Katri Hallamaa and Sanna Lepp{\"a}m{\"a}ki and Jaana M{\"a}tt{\"o} and Maria Saarela",

year = "2006",

doi = "10.1016/j.foodres.2005.05.006",

language = "English",

volume = "39",

pages = "22--32",

journal = "Food Research International",

issn = "0963-9969",

publisher = "Elsevier Ltd.",

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}

Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products. / Maukonen, Johanna (Corresponding Author); Alakomi, Hanna-Leena ; Nohynek, Liisa et al.
In: Food Research International, Vol. 39, No. 1, 2006, p. 22-32.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products

AU - Maukonen, Johanna

AU - Alakomi, Hanna-Leena

AU - Nohynek, Liisa

AU - Hallamaa, Katri

AU - Leppämäki, Sanna

AU - Mättö, Jaana

AU - Saarela, Maria

PY - 2006

Y1 - 2006

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AB - The suitability of the fluorescent techniques for the microbiological quality assessment of probiotic non-dairy drinks and probiotic pharmaceutical products was studied. The method optimization was first performed with pure cultures of probiotic strains detected in commercial products (representing Lactobacillus and Bifidobacterium species). Altogether seven different fluorescent viability stains combined with epifluorescence microscopy and flow cytometry were tested. The most applicable stains ChemChrome and LIVE/DEAD BacLight Viability Kit were further used for the viability studies of the commercial probiotic products. The studied products proved to be of good quality. All products contained the probiotic bacterium indicated on the label and the levels of living bacteria were reasonable per dose (108–1010 cells for suggested daily dose of pharmaceutical products and 108–109 cells per 100 ml of drink). The results obtained with fluorescent stains were mostly in agreement with results obtained with culture, rendering fluorescent techniques applicable alternatives for rapid viability assessment of probiotic products. Especially the newly developed fluorometry assay with BacLight proved to be fast and accurate.

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KW - Bifidobacterium

KW - Epifluorescence microscopy

KW - Flow cytometry

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DO - 10.1016/j.foodres.2005.05.006

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EP - 32

JO - Food Research International

JF - Food Research International

IS - 1

ER -

Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products

Abstract

Keywords

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