Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products

Johanna Maukonen (Corresponding Author), Hanna-Leena Alakomi, Liisa Nohynek, Katri Hallamaa, Sanna Leppämäki, Jaana Mättö, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

41 Citations (Scopus)

Abstract

The suitability of the fluorescent techniques for the microbiological quality assessment of probiotic non-dairy drinks and probiotic pharmaceutical products was studied. The method optimization was first performed with pure cultures of probiotic strains detected in commercial products (representing Lactobacillus and Bifidobacterium species). Altogether seven different fluorescent viability stains combined with epifluorescence microscopy and flow cytometry were tested. The most applicable stains ChemChrome and LIVE/DEAD BacLight Viability Kit were further used for the viability studies of the commercial probiotic products. The studied products proved to be of good quality. All products contained the probiotic bacterium indicated on the label and the levels of living bacteria were reasonable per dose (108–1010 cells for suggested daily dose of pharmaceutical products and 108–109 cells per 100 ml of drink). The results obtained with fluorescent stains were mostly in agreement with results obtained with culture, rendering fluorescent techniques applicable alternatives for rapid viability assessment of probiotic products. Especially the newly developed fluorometry assay with BacLight proved to be fast and accurate.
Original languageEnglish
Pages (from-to)22-32
Number of pages11
JournalFood Research International
Volume39
Issue number1
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Probiotics
probiotics
Bacteria
drugs
bacteria
viability
Pharmaceutical Preparations
Coloring Agents
methodology
Microbiological Techniques
Fluorometry
fluorometry
Bifidobacterium
system optimization
rendering
Lactobacillus
microbiological quality
dosage
flow cytometry
Microscopy

Keywords

  • Probiotic product
  • Lactobacillus
  • Bifidobacterium
  • Epifluorescence microscopy
  • Flow cytometry
  • Fluorometry assay

Cite this

@article{df78b59a89004e319fdb68613775c5d4,
title = "Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products",
abstract = "The suitability of the fluorescent techniques for the microbiological quality assessment of probiotic non-dairy drinks and probiotic pharmaceutical products was studied. The method optimization was first performed with pure cultures of probiotic strains detected in commercial products (representing Lactobacillus and Bifidobacterium species). Altogether seven different fluorescent viability stains combined with epifluorescence microscopy and flow cytometry were tested. The most applicable stains ChemChrome and LIVE/DEAD BacLight Viability Kit were further used for the viability studies of the commercial probiotic products. The studied products proved to be of good quality. All products contained the probiotic bacterium indicated on the label and the levels of living bacteria were reasonable per dose (108–1010 cells for suggested daily dose of pharmaceutical products and 108–109 cells per 100 ml of drink). The results obtained with fluorescent stains were mostly in agreement with results obtained with culture, rendering fluorescent techniques applicable alternatives for rapid viability assessment of probiotic products. Especially the newly developed fluorometry assay with BacLight proved to be fast and accurate.",
keywords = "Probiotic product, Lactobacillus, Bifidobacterium, Epifluorescence microscopy, Flow cytometry, Fluorometry assay",
author = "Johanna Maukonen and Hanna-Leena Alakomi and Liisa Nohynek and Katri Hallamaa and Sanna Lepp{\"a}m{\"a}ki and Jaana M{\"a}tt{\"o} and Maria Saarela",
year = "2006",
doi = "10.1016/j.foodres.2005.05.006",
language = "English",
volume = "39",
pages = "22--32",
journal = "Food Research International",
issn = "0963-9969",
publisher = "Elsevier",
number = "1",

}

Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products. / Maukonen, Johanna (Corresponding Author); Alakomi, Hanna-Leena; Nohynek, Liisa; Hallamaa, Katri; Leppämäki, Sanna; Mättö, Jaana; Saarela, Maria.

In: Food Research International, Vol. 39, No. 1, 2006, p. 22-32.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Suitability of the fluorescent techniques for the enumeration of probiotic bacteria in commercial non-dairy drinks and in pharmaceutical products

AU - Maukonen, Johanna

AU - Alakomi, Hanna-Leena

AU - Nohynek, Liisa

AU - Hallamaa, Katri

AU - Leppämäki, Sanna

AU - Mättö, Jaana

AU - Saarela, Maria

PY - 2006

Y1 - 2006

N2 - The suitability of the fluorescent techniques for the microbiological quality assessment of probiotic non-dairy drinks and probiotic pharmaceutical products was studied. The method optimization was first performed with pure cultures of probiotic strains detected in commercial products (representing Lactobacillus and Bifidobacterium species). Altogether seven different fluorescent viability stains combined with epifluorescence microscopy and flow cytometry were tested. The most applicable stains ChemChrome and LIVE/DEAD BacLight Viability Kit were further used for the viability studies of the commercial probiotic products. The studied products proved to be of good quality. All products contained the probiotic bacterium indicated on the label and the levels of living bacteria were reasonable per dose (108–1010 cells for suggested daily dose of pharmaceutical products and 108–109 cells per 100 ml of drink). The results obtained with fluorescent stains were mostly in agreement with results obtained with culture, rendering fluorescent techniques applicable alternatives for rapid viability assessment of probiotic products. Especially the newly developed fluorometry assay with BacLight proved to be fast and accurate.

AB - The suitability of the fluorescent techniques for the microbiological quality assessment of probiotic non-dairy drinks and probiotic pharmaceutical products was studied. The method optimization was first performed with pure cultures of probiotic strains detected in commercial products (representing Lactobacillus and Bifidobacterium species). Altogether seven different fluorescent viability stains combined with epifluorescence microscopy and flow cytometry were tested. The most applicable stains ChemChrome and LIVE/DEAD BacLight Viability Kit were further used for the viability studies of the commercial probiotic products. The studied products proved to be of good quality. All products contained the probiotic bacterium indicated on the label and the levels of living bacteria were reasonable per dose (108–1010 cells for suggested daily dose of pharmaceutical products and 108–109 cells per 100 ml of drink). The results obtained with fluorescent stains were mostly in agreement with results obtained with culture, rendering fluorescent techniques applicable alternatives for rapid viability assessment of probiotic products. Especially the newly developed fluorometry assay with BacLight proved to be fast and accurate.

KW - Probiotic product

KW - Lactobacillus

KW - Bifidobacterium

KW - Epifluorescence microscopy

KW - Flow cytometry

KW - Fluorometry assay

U2 - 10.1016/j.foodres.2005.05.006

DO - 10.1016/j.foodres.2005.05.006

M3 - Article

VL - 39

SP - 22

EP - 32

JO - Food Research International

JF - Food Research International

SN - 0963-9969

IS - 1

ER -