Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto: Analyzed by Quantitative PCR

Merja Itävaara, Maija-Leena Vehkomäki, Aura Nousiainen

Research output: Book/ReportReport

Abstract

The GEOFUNC Project is connected to safety and risk assessment research on the final disposal site of nuclear waste disposal. Sulphate-reducing micro-organisms, which are the major group of micro-organisms of concern, are able to cause copper corrosion by converting sulphate to sulphide, which is a very corrosive agent. The quantitative assessment of sulphate reducers by the qPCR method, which is based on sulphite reductase gene quantification, is related to the transformation of sulphate to sulphide. In this project we developed external and internal standard methods for our qPCR method in order to evaluate the quantitative amount and efficiency of DNA extractions and PCR inhibition possibly caused by high salt or iron concentrations. These standards are needed in quantifying and estimating the real quantity of sulphate reducers on the basis of their functional genes. Sulphate reducers were detected in all the samples studied. Based on this small dataset, no clear relationships were found between the geochemistry and the numbers of sulphate reducers, and the phenomena should be studied in more detail with more variable drillhole waters in the future.
Original languageEnglish
Place of PublicationEurajoki
PublisherPosiva
Number of pages50
Publication statusPublished - 2008
MoE publication typeD4 Published development or research report or study

Publication series

SeriesPosiva Working Report
Number2008-82

Fingerprint

sulfate-reducing bacterium
sulfate
groundwater
sulfide
gene
sulfite
waste disposal
radioactive waste
corrosion
risk assessment
geochemistry
safety
copper
salt
DNA
iron
method

Keywords

  • Sulphate-reducing bacteria
  • quantitative PCR
  • Olkiluoto
  • Onkalo

Cite this

Itävaara, M., Vehkomäki, M-L., & Nousiainen, A. (2008). Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto: Analyzed by Quantitative PCR. Eurajoki: Posiva . Posiva Working Report, No. 2008-82
Itävaara, Merja ; Vehkomäki, Maija-Leena ; Nousiainen, Aura. / Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto : Analyzed by Quantitative PCR. Eurajoki : Posiva , 2008. 50 p. (Posiva Working Report; No. 2008-82).
@book{d65d841d739a4605871a5988332b4333,
title = "Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto: Analyzed by Quantitative PCR",
abstract = "The GEOFUNC Project is connected to safety and risk assessment research on the final disposal site of nuclear waste disposal. Sulphate-reducing micro-organisms, which are the major group of micro-organisms of concern, are able to cause copper corrosion by converting sulphate to sulphide, which is a very corrosive agent. The quantitative assessment of sulphate reducers by the qPCR method, which is based on sulphite reductase gene quantification, is related to the transformation of sulphate to sulphide. In this project we developed external and internal standard methods for our qPCR method in order to evaluate the quantitative amount and efficiency of DNA extractions and PCR inhibition possibly caused by high salt or iron concentrations. These standards are needed in quantifying and estimating the real quantity of sulphate reducers on the basis of their functional genes. Sulphate reducers were detected in all the samples studied. Based on this small dataset, no clear relationships were found between the geochemistry and the numbers of sulphate reducers, and the phenomena should be studied in more detail with more variable drillhole waters in the future.",
keywords = "Sulphate-reducing bacteria, quantitative PCR, Olkiluoto, Onkalo",
author = "Merja It{\"a}vaara and Maija-Leena Vehkom{\"a}ki and Aura Nousiainen",
year = "2008",
language = "English",
series = "Posiva Working Report",
publisher = "Posiva",
number = "2008-82",
address = "Finland",

}

Itävaara, M, Vehkomäki, M-L & Nousiainen, A 2008, Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto: Analyzed by Quantitative PCR. Posiva Working Report, no. 2008-82, Posiva , Eurajoki.

Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto : Analyzed by Quantitative PCR. / Itävaara, Merja; Vehkomäki, Maija-Leena; Nousiainen, Aura.

Eurajoki : Posiva , 2008. 50 p. (Posiva Working Report; No. 2008-82).

Research output: Book/ReportReport

TY - BOOK

T1 - Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto

T2 - Analyzed by Quantitative PCR

AU - Itävaara, Merja

AU - Vehkomäki, Maija-Leena

AU - Nousiainen, Aura

PY - 2008

Y1 - 2008

N2 - The GEOFUNC Project is connected to safety and risk assessment research on the final disposal site of nuclear waste disposal. Sulphate-reducing micro-organisms, which are the major group of micro-organisms of concern, are able to cause copper corrosion by converting sulphate to sulphide, which is a very corrosive agent. The quantitative assessment of sulphate reducers by the qPCR method, which is based on sulphite reductase gene quantification, is related to the transformation of sulphate to sulphide. In this project we developed external and internal standard methods for our qPCR method in order to evaluate the quantitative amount and efficiency of DNA extractions and PCR inhibition possibly caused by high salt or iron concentrations. These standards are needed in quantifying and estimating the real quantity of sulphate reducers on the basis of their functional genes. Sulphate reducers were detected in all the samples studied. Based on this small dataset, no clear relationships were found between the geochemistry and the numbers of sulphate reducers, and the phenomena should be studied in more detail with more variable drillhole waters in the future.

AB - The GEOFUNC Project is connected to safety and risk assessment research on the final disposal site of nuclear waste disposal. Sulphate-reducing micro-organisms, which are the major group of micro-organisms of concern, are able to cause copper corrosion by converting sulphate to sulphide, which is a very corrosive agent. The quantitative assessment of sulphate reducers by the qPCR method, which is based on sulphite reductase gene quantification, is related to the transformation of sulphate to sulphide. In this project we developed external and internal standard methods for our qPCR method in order to evaluate the quantitative amount and efficiency of DNA extractions and PCR inhibition possibly caused by high salt or iron concentrations. These standards are needed in quantifying and estimating the real quantity of sulphate reducers on the basis of their functional genes. Sulphate reducers were detected in all the samples studied. Based on this small dataset, no clear relationships were found between the geochemistry and the numbers of sulphate reducers, and the phenomena should be studied in more detail with more variable drillhole waters in the future.

KW - Sulphate-reducing bacteria

KW - quantitative PCR

KW - Olkiluoto

KW - Onkalo

M3 - Report

T3 - Posiva Working Report

BT - Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto

PB - Posiva

CY - Eurajoki

ER -

Itävaara M, Vehkomäki M-L, Nousiainen A. Sulphate-Reducing Bacteria in Ground Water Samples from Olkiluoto: Analyzed by Quantitative PCR. Eurajoki: Posiva , 2008. 50 p. (Posiva Working Report; No. 2008-82).