The purpose of the present work was to study the extraction of 1-butanol from aqueous solutions using supercritical carbon dioxide at 100 bar and 40°C as a solvent. Experimental studies were performed using a 35 mm diameter mechanically agitated Oldshue–Rushton-type supercritical fluid extraction column. Over 99.7% of the initial amount of 1-butanol was removed from the feed stream when the solvent-to-feed ratio was 2.7. The lowest 1-butanol concentration in the raffinate was approximately 0.02 wt%, and the extract contained typically 85–90 wt% 1-butanol. The values of the overall mass transfer coefficient Koda were in the range 0.0019–0.0034 s−1, and the values of the height equivalent to a theoretical stage HETS ranged from 0.4 to 0.5 m. Mechanical agitation had no effect on the column efficiency at the selected extraction conditions.