Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose

Lasse K. Tolonen; Minna Juvonen; Klaus Niemelä; Atte Mikkelson; Maija Tenkanen; Herbert Sixta

doi:10.1016/j.carres.2014.10.012

Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose

Lasse K. Tolonen, Minna Juvonen, Klaus Niemelä, Atte Mikkelson, Maija Tenkanen, Herbert Sixta

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.

Original language	English
Pages (from-to)	16-23
Journal	Carbohydrate Research
Volume	401
DOIs	https://doi.org/10.1016/j.carres.2014.10.012
Publication status	Published - 2015
MoE publication type	A1 Journal article-refereed

Keywords

Biorefinery
Supercritical water
Cellulose
Oligosaccharide
Prebiotics

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10.1016/j.carres.2014.10.012

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title = "Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose",

abstract = "Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.",

keywords = "Biorefinery, Supercritical water, Cellulose, Oligosaccharide, Prebiotics",

author = "Tolonen, {Lasse K.} and Minna Juvonen and Klaus Niemel{\"a} and Atte Mikkelson and Maija Tenkanen and Herbert Sixta",

year = "2015",

doi = "10.1016/j.carres.2014.10.012",

language = "English",

volume = "401",

pages = "16--23",

journal = "Carbohydrate Research",

issn = "0008-6215",

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TY - JOUR

T1 - Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose

AU - Tolonen, Lasse K.

AU - Juvonen, Minna

AU - Niemelä, Klaus

AU - Mikkelson, Atte

AU - Tenkanen, Maija

AU - Sixta, Herbert

PY - 2015

Y1 - 2015

N2 - Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.

AB - Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.

KW - Biorefinery

KW - Supercritical water

KW - Cellulose

KW - Oligosaccharide

KW - Prebiotics

U2 - 10.1016/j.carres.2014.10.012

DO - 10.1016/j.carres.2014.10.012

M3 - Article

SN - 0008-6215

VL - 401

SP - 16

EP - 23

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -

Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose

Abstract

Keywords

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