Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose

Lasse K. Tolonen, Minna Juvonen, Klaus Niemelä, Atte Mikkelson, Maija Tenkanen, Herbert Sixta

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)

Abstract

Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.
Original languageEnglish
Pages (from-to)16-23
JournalCarbohydrate Research
Volume401
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

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Water Purification
Oligosaccharides
Water treatment
Degradation
Water
Precipitates
Monosaccharides
Molar mass
Dehydration
Reaction products
Sugars
Pressure
microcrystalline cellulose

Keywords

  • Biorefinery
  • Supercritical water
  • Cellulose
  • Oligosaccharide
  • Prebiotics

Cite this

Tolonen, Lasse K. ; Juvonen, Minna ; Niemelä, Klaus ; Mikkelson, Atte ; Tenkanen, Maija ; Sixta, Herbert. / Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose. In: Carbohydrate Research. 2015 ; Vol. 401. pp. 16-23.
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abstract = "Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt {\%} for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt {\%} ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt {\%} monomeric sugars, and 37 wt {\%} unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.",
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Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose. / Tolonen, Lasse K.; Juvonen, Minna; Niemelä, Klaus; Mikkelson, Atte; Tenkanen, Maija; Sixta, Herbert.

In: Carbohydrate Research, Vol. 401, 2015, p. 16-23.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose

AU - Tolonen, Lasse K.

AU - Juvonen, Minna

AU - Niemelä, Klaus

AU - Mikkelson, Atte

AU - Tenkanen, Maija

AU - Sixta, Herbert

PY - 2015

Y1 - 2015

N2 - Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.

AB - Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.

KW - Biorefinery

KW - Supercritical water

KW - Cellulose

KW - Oligosaccharide

KW - Prebiotics

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DO - 10.1016/j.carres.2014.10.012

M3 - Article

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