Supercritical water treatment for cello-oligosaccharide production from microcrystalline cellulose

Lasse K. Tolonen, Minna Juvonen, Klaus Niemelä, Atte Mikkelson, Maija Tenkanen, Herbert Sixta

    Research output: Contribution to journalArticleScientificpeer-review

    49 Citations (Scopus)


    Microcrystalline cellulose was treated in supercritical water at 380 °C and at a pressure of 250 bar for 0.2, 0.4, and 0.6 s. The yield of the ambient-water-insoluble precipitate and its average molar mass decreased with an extended treatment time. The highest yield of 42 wt % for DP2-9 cello-oligosaccharides was achieved after the 0.4 s treatment. The reaction products included also 11 wt % ambient-water-insoluble precipitate with a DPw of 16, and 6.1 wt % monomeric sugars, and 37 wt % unidentified degradation products. Oligo- and monosaccharide-derived dehydration and retro-aldol fragmentation products were analyzed via a combination of HPAEC-PAD-MS, ESI-MS/MS, and GC-MS techniques. The total amount of degradation products increased with treatment time, and fragmented (glucosyln-erythrose, glucosyln-glycolaldehyde), and dehydrated (glucosyln-levoglucosan) were identified as the main oligomeric degradation products from the cello-oligosaccharides.
    Original languageEnglish
    Pages (from-to)16-23
    JournalCarbohydrate Research
    Publication statusPublished - 2015
    MoE publication typeA1 Journal article-refereed


    • Biorefinery
    • Supercritical water
    • Cellulose
    • Oligosaccharide
    • Prebiotics


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