TY - JOUR
T1 - Surface-enhanced Raman spectroscopy for identification and discrimination of beverage spoilage yeasts using patterned substrates and gold nanoparticles
AU - Uusitalo, Sanna
AU - Popov, Alexey
AU - Ryabchikov, Yury V.
AU - Bibikova, Olga
AU - Alakomi, Hanna-Leena
AU - Juvonen, Riikka
AU - Kontturi, Ville
AU - Siitonen, Samuli
AU - Kabashin, Andrei
AU - Meglinski, Igor
AU - Hiltunen, Jussi
AU - Laitila, Arja
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/11/1
Y1 - 2017/11/1
N2 - In the beverage industry, the detection of spoilage yeasts such as Wickerhamomyces anomalus and Brettanomyces bruxellensis can be labourious and time-consuming. In the present study, a simple and repeatable technique was developed for rapid yeast detection using a combination of patterned gold-coated surface-enhanced Raman spectroscopy (SERS) substrates and gold nanoparticles. W. anomalus and B. bruxellensis showed several characteristic peaks, enabling the discrimination of these yeasts without chemometric analysis. The control yeast used as an indicator yeast, Rhodotorula mucilaginosa, showed 7 cell wall-related peaks originating from lipids and haemoproteins. Analysing W. anomalus SERS spectra with differently sized and shaped gold nanoparticles revealed the benefit of using either large, spherical, chemically synthesised gold nanoparticles or small, laser-synthesised, gold-silicon nanoparticles for yeast detection. Additionally, the spectra showed differences in SERS signal construction for small molecules and biological cells, as the nanoparticles with best response in biological cell detection did not excel in small molecule detection. The use of small composite gold-silicon nanoparticles in combination with the SERS substrate gave distinctive spectra for all detected yeast species.
AB - In the beverage industry, the detection of spoilage yeasts such as Wickerhamomyces anomalus and Brettanomyces bruxellensis can be labourious and time-consuming. In the present study, a simple and repeatable technique was developed for rapid yeast detection using a combination of patterned gold-coated surface-enhanced Raman spectroscopy (SERS) substrates and gold nanoparticles. W. anomalus and B. bruxellensis showed several characteristic peaks, enabling the discrimination of these yeasts without chemometric analysis. The control yeast used as an indicator yeast, Rhodotorula mucilaginosa, showed 7 cell wall-related peaks originating from lipids and haemoproteins. Analysing W. anomalus SERS spectra with differently sized and shaped gold nanoparticles revealed the benefit of using either large, spherical, chemically synthesised gold nanoparticles or small, laser-synthesised, gold-silicon nanoparticles for yeast detection. Additionally, the spectra showed differences in SERS signal construction for small molecules and biological cells, as the nanoparticles with best response in biological cell detection did not excel in small molecule detection. The use of small composite gold-silicon nanoparticles in combination with the SERS substrate gave distinctive spectra for all detected yeast species.
KW - beverage
KW - yeast
KW - raman spectroscopy
KW - SERS
KW - nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85019565935&partnerID=8YFLogxK
U2 - 10.1016/j.jfoodeng.2017.05.007
DO - 10.1016/j.jfoodeng.2017.05.007
M3 - Article
SN - 0260-8774
VL - 212
SP - 47
EP - 54
JO - Journal of Food Engineering
JF - Journal of Food Engineering
ER -