TY - JOUR
T1 - Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnostics
AU - Orelma, Hannes
AU - Filpponen, Ilari
AU - Johansson, Leena Sisko
AU - Österberg, Monika
AU - Rojas, Orlando J.
AU - Laine, Janne
PY - 2012/1/1
Y1 - 2012/1/1
N2 - We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/ NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.
AB - We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/ NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.
UR - http://www.scopus.com/inward/record.url?scp=84873517464&partnerID=8YFLogxK
U2 - 10.1007/s13758-012-0061-7
DO - 10.1007/s13758-012-0061-7
M3 - Article
C2 - 23055097
AN - SCOPUS:84873517464
SN - 1934-8630
VL - 7
SP - 1
EP - 12
JO - Biointerphases
JF - Biointerphases
IS - 1
ER -