Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnostics

Hannes Orelma, Ilari Filpponen, Leena Sisko Johansson, Monika Österberg, Orlando J. Rojas, Janne Laine

Research output: Contribution to journalArticleScientificpeer-review

77 Citations (Scopus)

Abstract

We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/ NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalBiointerphases
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Jan 2012
MoE publication typeA1 Journal article-refereed

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Cellulose films
immunoassay
cellulose
Immunoassay
Cellulose
platforms
Immunoglobulin G
Carboxylation
Conductometry
Water
Hydrogel
Quartz crystal microbalances
Fluorescence microscopy
carboxylation
Quartz Crystal Microbalance Techniques
Angle measurement
Bovine Serum Albumin
microscopy
Titration
Hydrogels

Cite this

Orelma, Hannes ; Filpponen, Ilari ; Johansson, Leena Sisko ; Österberg, Monika ; Rojas, Orlando J. ; Laine, Janne. / Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnostics. In: Biointerphases. 2012 ; Vol. 7, No. 1. pp. 1-12.
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abstract = "We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/ NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.",
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Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnostics. / Orelma, Hannes; Filpponen, Ilari; Johansson, Leena Sisko; Österberg, Monika; Rojas, Orlando J.; Laine, Janne.

In: Biointerphases, Vol. 7, No. 1, 01.01.2012, p. 1-12.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Orelma, Hannes

AU - Filpponen, Ilari

AU - Johansson, Leena Sisko

AU - Österberg, Monika

AU - Rojas, Orlando J.

AU - Laine, Janne

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AB - We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/ NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.

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