Surface properties and interaction forces of biopolymer-doped conductive polypyrrole surfaces by atomic force microscopy

Jani Pelto (Corresponding Author), S.P. Haimi, Aliisa Silander, S.S. Miettinen, Kirsi Tappura, M.J. Higgins, G.G. Wallace

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Surface properties and electrical charges are critical factors elucidating cell interactions on biomaterial surfaces. The surface potential distribution and the nanoscopic and microscopic surface elasticity of organic polypyrrole–hyaluronic acid (PPy-HA) were studied by atomic force microscopy (AFM) in a fluid environment in order to explain the observed enhancement in the attachment of human adipose stem cells on positively charged PPy-HA films. The electrostatic force between the AFM tip and a charged PPy-HA surface, the tip–sample adhesion force, and elastic moduli were estimated from the AFM force curves, and the data were fitted to electrostatic double-layer and elastic contact models. The surface potential of the charged and dried PPy-HA films was assessed with Kelvin probe force microscopy (KPFM), and the KPFM data were correlated to the fluid AFM data. The surface charge distribution and elasticity were both found to correlate well with the nodular morphology of PPy-HA and to be sensitive to the electrochemical charging conditions. Furthermore, a significant change in the adhesion was detected when the surface was electrochemically charged positive. The results highlight the potential of positively charged PPy-HA as a coating material to enhance the stem cell response in tissue-engineering scaffolds.
Original languageEnglish
Pages (from-to)6099-6108
Number of pages9
JournalLangmuir
Volume29
Issue number20
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Biopolymers
biopolymers
polypyrroles
Polypyrroles
surface properties
surface reactions
Surface properties
Atomic force microscopy
atomic force microscopy
Acids
acids
Surface potential
Stem cells
Elasticity
Microscopic examination
stem cells
Adhesion
Tissue Scaffolds
Fluids
Electrostatic force

Cite this

Pelto, Jani ; Haimi, S.P. ; Silander, Aliisa ; Miettinen, S.S. ; Tappura, Kirsi ; Higgins, M.J. ; Wallace, G.G. / Surface properties and interaction forces of biopolymer-doped conductive polypyrrole surfaces by atomic force microscopy. In: Langmuir. 2013 ; Vol. 29, No. 20. pp. 6099-6108.
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title = "Surface properties and interaction forces of biopolymer-doped conductive polypyrrole surfaces by atomic force microscopy",
abstract = "Surface properties and electrical charges are critical factors elucidating cell interactions on biomaterial surfaces. The surface potential distribution and the nanoscopic and microscopic surface elasticity of organic polypyrrole–hyaluronic acid (PPy-HA) were studied by atomic force microscopy (AFM) in a fluid environment in order to explain the observed enhancement in the attachment of human adipose stem cells on positively charged PPy-HA films. The electrostatic force between the AFM tip and a charged PPy-HA surface, the tip–sample adhesion force, and elastic moduli were estimated from the AFM force curves, and the data were fitted to electrostatic double-layer and elastic contact models. The surface potential of the charged and dried PPy-HA films was assessed with Kelvin probe force microscopy (KPFM), and the KPFM data were correlated to the fluid AFM data. The surface charge distribution and elasticity were both found to correlate well with the nodular morphology of PPy-HA and to be sensitive to the electrochemical charging conditions. Furthermore, a significant change in the adhesion was detected when the surface was electrochemically charged positive. The results highlight the potential of positively charged PPy-HA as a coating material to enhance the stem cell response in tissue-engineering scaffolds.",
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Surface properties and interaction forces of biopolymer-doped conductive polypyrrole surfaces by atomic force microscopy. / Pelto, Jani (Corresponding Author); Haimi, S.P.; Silander, Aliisa; Miettinen, S.S.; Tappura, Kirsi; Higgins, M.J.; Wallace, G.G.

In: Langmuir, Vol. 29, No. 20, 2013, p. 6099-6108.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Surface properties and interaction forces of biopolymer-doped conductive polypyrrole surfaces by atomic force microscopy

AU - Pelto, Jani

AU - Haimi, S.P.

AU - Silander, Aliisa

AU - Miettinen, S.S.

AU - Tappura, Kirsi

AU - Higgins, M.J.

AU - Wallace, G.G.

PY - 2013

Y1 - 2013

N2 - Surface properties and electrical charges are critical factors elucidating cell interactions on biomaterial surfaces. The surface potential distribution and the nanoscopic and microscopic surface elasticity of organic polypyrrole–hyaluronic acid (PPy-HA) were studied by atomic force microscopy (AFM) in a fluid environment in order to explain the observed enhancement in the attachment of human adipose stem cells on positively charged PPy-HA films. The electrostatic force between the AFM tip and a charged PPy-HA surface, the tip–sample adhesion force, and elastic moduli were estimated from the AFM force curves, and the data were fitted to electrostatic double-layer and elastic contact models. The surface potential of the charged and dried PPy-HA films was assessed with Kelvin probe force microscopy (KPFM), and the KPFM data were correlated to the fluid AFM data. The surface charge distribution and elasticity were both found to correlate well with the nodular morphology of PPy-HA and to be sensitive to the electrochemical charging conditions. Furthermore, a significant change in the adhesion was detected when the surface was electrochemically charged positive. The results highlight the potential of positively charged PPy-HA as a coating material to enhance the stem cell response in tissue-engineering scaffolds.

AB - Surface properties and electrical charges are critical factors elucidating cell interactions on biomaterial surfaces. The surface potential distribution and the nanoscopic and microscopic surface elasticity of organic polypyrrole–hyaluronic acid (PPy-HA) were studied by atomic force microscopy (AFM) in a fluid environment in order to explain the observed enhancement in the attachment of human adipose stem cells on positively charged PPy-HA films. The electrostatic force between the AFM tip and a charged PPy-HA surface, the tip–sample adhesion force, and elastic moduli were estimated from the AFM force curves, and the data were fitted to electrostatic double-layer and elastic contact models. The surface potential of the charged and dried PPy-HA films was assessed with Kelvin probe force microscopy (KPFM), and the KPFM data were correlated to the fluid AFM data. The surface charge distribution and elasticity were both found to correlate well with the nodular morphology of PPy-HA and to be sensitive to the electrochemical charging conditions. Furthermore, a significant change in the adhesion was detected when the surface was electrochemically charged positive. The results highlight the potential of positively charged PPy-HA as a coating material to enhance the stem cell response in tissue-engineering scaffolds.

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