Survival of potentially probiotic enterococci in dairy matrices and in the human gastrointestinal tract

L.L. Pimentel, Jaana Mättö, F.X. Malcata, M.E. Pintado (Corresponding Author), Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

The aim of this study was to investigate the stability of Enterococcus strains isolated from a traditional Portuguese cheese and previously proved to be safe, in dairy matrices, and to assess survival of the best strains in the human gastrointestinal (GI) tract. Enterococcus faecium 32 and Enterococcus durans 37 were added to yoghurt that was ingested by 4 healthy adults. Detection of the enterococcal strains was performed with RAPD-PCR. The intervention trial showed transient colonisation with both strains, via presence in faeces during the ingestion period and disappearance by 10 d post-ingestion. Viable numbers of enterococci increased during the consumption period by 1.8–4.4 log-values, and returned to baseline level during the follow-up period. Based on data of the dairy matrix stability trials and human intervention study involving yoghurt ingestion, E. faecium 32 survived well both in the food matrix and in the human GI tract, thus showing probiotic potential.
Original languageEnglish
Pages (from-to)53-57
Number of pages4
JournalInternational Dairy Journal
Volume27
Issue number1-2
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Enterococcus
Probiotics
probiotics
gastrointestinal system
Gastrointestinal Tract
Enterococcus faecium
dairies
Yogurt
Eating
Survival
ingestion
yogurt
Random Amplified Polymorphic DNA Technique
Enterococcus durans
Cheese
Feces
food matrix
cheeses
Food
feces

Cite this

Pimentel, L.L. ; Mättö, Jaana ; Malcata, F.X. ; Pintado, M.E. ; Saarela, Maria. / Survival of potentially probiotic enterococci in dairy matrices and in the human gastrointestinal tract. In: International Dairy Journal. 2012 ; Vol. 27, No. 1-2. pp. 53-57.
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abstract = "The aim of this study was to investigate the stability of Enterococcus strains isolated from a traditional Portuguese cheese and previously proved to be safe, in dairy matrices, and to assess survival of the best strains in the human gastrointestinal (GI) tract. Enterococcus faecium 32 and Enterococcus durans 37 were added to yoghurt that was ingested by 4 healthy adults. Detection of the enterococcal strains was performed with RAPD-PCR. The intervention trial showed transient colonisation with both strains, via presence in faeces during the ingestion period and disappearance by 10 d post-ingestion. Viable numbers of enterococci increased during the consumption period by 1.8–4.4 log-values, and returned to baseline level during the follow-up period. Based on data of the dairy matrix stability trials and human intervention study involving yoghurt ingestion, E. faecium 32 survived well both in the food matrix and in the human GI tract, thus showing probiotic potential.",
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Survival of potentially probiotic enterococci in dairy matrices and in the human gastrointestinal tract. / Pimentel, L.L.; Mättö, Jaana; Malcata, F.X.; Pintado, M.E. (Corresponding Author); Saarela, Maria.

In: International Dairy Journal, Vol. 27, No. 1-2, 2012, p. 53-57.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Survival of potentially probiotic enterococci in dairy matrices and in the human gastrointestinal tract

AU - Pimentel, L.L.

AU - Mättö, Jaana

AU - Malcata, F.X.

AU - Pintado, M.E.

AU - Saarela, Maria

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AB - The aim of this study was to investigate the stability of Enterococcus strains isolated from a traditional Portuguese cheese and previously proved to be safe, in dairy matrices, and to assess survival of the best strains in the human gastrointestinal (GI) tract. Enterococcus faecium 32 and Enterococcus durans 37 were added to yoghurt that was ingested by 4 healthy adults. Detection of the enterococcal strains was performed with RAPD-PCR. The intervention trial showed transient colonisation with both strains, via presence in faeces during the ingestion period and disappearance by 10 d post-ingestion. Viable numbers of enterococci increased during the consumption period by 1.8–4.4 log-values, and returned to baseline level during the follow-up period. Based on data of the dairy matrix stability trials and human intervention study involving yoghurt ingestion, E. faecium 32 survived well both in the food matrix and in the human GI tract, thus showing probiotic potential.

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JF - International Dairy Journal

SN - 0958-6946

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