Susceptibility of human and probiotic Bifidobacterium spp. to selected antibiotics as determined by the Etest method

Jaana Mättö, Angela H.A.M. van Hoek, Konrad J. Domig, Maria Saarela, Ana Belén Floréz, Elke Brockmann, Ernst Amtmann, Baltasar Mayo, Henk J.M. Aarts, Morten Danielsen

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Abstract

This study reports the antibiotic susceptibility of 203 strains representing human or probiotic associated Bifidobacterium species as determined by the Etest method. Strains showing minimum inhibitory concentration (MIC) for tetracycline ⩾16 μg mL−1 were detected in all studied Bifidobacterium species/groups (prevalence 4–18%).
Mostly the high MICs could be associated with a tet gene, although the correspondence between the phenotypic susceptibility and detection of tet determinants was not complete. In addition to tet(W), tet(O), which has not been previously described in bifidobacteria, was detected. Occasional erythromycin (2%) and/or clindamycin (5%) resistant strains were found, while the strains were uniformly susceptible to ampicillin and vancomycin.
Bifidobacterium strains displayed generally high MICs for streptomycin and gentamicin suggesting intrinsic resistance. The Etest on lactic acid bacteria susceptibility test medium supplemented with cysteine proved to be an applicable technique for antimicrobial susceptibility testing of bifidobacteria, and is especially useful when susceptibility data of individual strains is needed.
Original languageEnglish
Pages (from-to)1123-1131
JournalInternational Dairy Journal
Volume17
Issue number9
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Disk Diffusion Antimicrobial Tests
Bifidobacterium
Probiotics
probiotics
antibiotics
Anti-Bacterial Agents
methodology
Clindamycin
Microbial Sensitivity Tests
Streptomycin
clindamycin
Erythromycin
Vancomycin
Ampicillin
Gentamicins
Tetracycline
vancomycin
erythromycin
Cysteine
gentamicin

Keywords

  • bifidobacterium
  • antimicrobial susceptibility
  • tetracycline
  • antibiotics
  • probiotics
  • human intestinal

Cite this

Mättö, J., van Hoek, A. H. A. M., Domig, K. J., Saarela, M., Floréz, A. B., Brockmann, E., ... Danielsen, M. (2007). Susceptibility of human and probiotic Bifidobacterium spp. to selected antibiotics as determined by the Etest method. International Dairy Journal, 17(9), 1123-1131. https://doi.org/10.1016/j.idairyj.2007.01.008
Mättö, Jaana ; van Hoek, Angela H.A.M. ; Domig, Konrad J. ; Saarela, Maria ; Floréz, Ana Belén ; Brockmann, Elke ; Amtmann, Ernst ; Mayo, Baltasar ; Aarts, Henk J.M. ; Danielsen, Morten. / Susceptibility of human and probiotic Bifidobacterium spp. to selected antibiotics as determined by the Etest method. In: International Dairy Journal. 2007 ; Vol. 17, No. 9. pp. 1123-1131.
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abstract = "This study reports the antibiotic susceptibility of 203 strains representing human or probiotic associated Bifidobacterium species as determined by the Etest method. Strains showing minimum inhibitory concentration (MIC) for tetracycline ⩾16 μg mL−1 were detected in all studied Bifidobacterium species/groups (prevalence 4–18{\%}). Mostly the high MICs could be associated with a tet gene, although the correspondence between the phenotypic susceptibility and detection of tet determinants was not complete. In addition to tet(W), tet(O), which has not been previously described in bifidobacteria, was detected. Occasional erythromycin (2{\%}) and/or clindamycin (5{\%}) resistant strains were found, while the strains were uniformly susceptible to ampicillin and vancomycin. Bifidobacterium strains displayed generally high MICs for streptomycin and gentamicin suggesting intrinsic resistance. The Etest on lactic acid bacteria susceptibility test medium supplemented with cysteine proved to be an applicable technique for antimicrobial susceptibility testing of bifidobacteria, and is especially useful when susceptibility data of individual strains is needed.",
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Mättö, J, van Hoek, AHAM, Domig, KJ, Saarela, M, Floréz, AB, Brockmann, E, Amtmann, E, Mayo, B, Aarts, HJM & Danielsen, M 2007, 'Susceptibility of human and probiotic Bifidobacterium spp. to selected antibiotics as determined by the Etest method', International Dairy Journal, vol. 17, no. 9, pp. 1123-1131. https://doi.org/10.1016/j.idairyj.2007.01.008

Susceptibility of human and probiotic Bifidobacterium spp. to selected antibiotics as determined by the Etest method. / Mättö, Jaana; van Hoek, Angela H.A.M.; Domig, Konrad J.; Saarela, Maria; Floréz, Ana Belén; Brockmann, Elke; Amtmann, Ernst; Mayo, Baltasar; Aarts, Henk J.M.; Danielsen, Morten.

In: International Dairy Journal, Vol. 17, No. 9, 2007, p. 1123-1131.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Mättö, Jaana

AU - van Hoek, Angela H.A.M.

AU - Domig, Konrad J.

AU - Saarela, Maria

AU - Floréz, Ana Belén

AU - Brockmann, Elke

AU - Amtmann, Ernst

AU - Mayo, Baltasar

AU - Aarts, Henk J.M.

AU - Danielsen, Morten

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N2 - This study reports the antibiotic susceptibility of 203 strains representing human or probiotic associated Bifidobacterium species as determined by the Etest method. Strains showing minimum inhibitory concentration (MIC) for tetracycline ⩾16 μg mL−1 were detected in all studied Bifidobacterium species/groups (prevalence 4–18%). Mostly the high MICs could be associated with a tet gene, although the correspondence between the phenotypic susceptibility and detection of tet determinants was not complete. In addition to tet(W), tet(O), which has not been previously described in bifidobacteria, was detected. Occasional erythromycin (2%) and/or clindamycin (5%) resistant strains were found, while the strains were uniformly susceptible to ampicillin and vancomycin. Bifidobacterium strains displayed generally high MICs for streptomycin and gentamicin suggesting intrinsic resistance. The Etest on lactic acid bacteria susceptibility test medium supplemented with cysteine proved to be an applicable technique for antimicrobial susceptibility testing of bifidobacteria, and is especially useful when susceptibility data of individual strains is needed.

AB - This study reports the antibiotic susceptibility of 203 strains representing human or probiotic associated Bifidobacterium species as determined by the Etest method. Strains showing minimum inhibitory concentration (MIC) for tetracycline ⩾16 μg mL−1 were detected in all studied Bifidobacterium species/groups (prevalence 4–18%). Mostly the high MICs could be associated with a tet gene, although the correspondence between the phenotypic susceptibility and detection of tet determinants was not complete. In addition to tet(W), tet(O), which has not been previously described in bifidobacteria, was detected. Occasional erythromycin (2%) and/or clindamycin (5%) resistant strains were found, while the strains were uniformly susceptible to ampicillin and vancomycin. Bifidobacterium strains displayed generally high MICs for streptomycin and gentamicin suggesting intrinsic resistance. The Etest on lactic acid bacteria susceptibility test medium supplemented with cysteine proved to be an applicable technique for antimicrobial susceptibility testing of bifidobacteria, and is especially useful when susceptibility data of individual strains is needed.

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KW - tetracycline

KW - antibiotics

KW - probiotics

KW - human intestinal

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