Susceptibility of pretreated wood sections of Norway spruce (Picea abies) clones to enzymatic hydrolysis

Y.P. Rodriguez (Corresponding Author), H. Puhakka-Tarvainen, O. Pastinen, Matti Siika-aho, L. Alvila, O. Turunen, L. Morales, A. Pappinen

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

The structure of softwoods, which confers resistance to degradation through hydrolysis and decay, currently limits their use for the production of biofuels. However, since wood is very heterogeneous, it is possible that differences in wood properties within and between trees could differentially affect its processability. In this research, heartwood (inner) and sapwood (outer) from Norway spruce (Picea abies (L.) Karst.) clones were enzymatically hydrolyzed by Trichoderma viride cellulases after concentrated acid pretreatment. Wood sections with two particle sizes were compared based on their susceptibility to enzymatic hydrolysis, evaluated by assaying the formation of hydrolysis products and measured as reducing sugar yield (RSY). We also studied the relationship between RSY and the susceptibility to Heterobasidion parviporum wood decay and whether these traits are reflected in wood density and yield. Wood from the outer section produced more RSY with higher glucan but lower lignin content than wood from the inner section. Furthermore, susceptibility to enzymatic hydrolysis was positively correlated with H. parviporum wood decay, while both processes were negatively correlated with wood density. Our results revealed the importance of clonal trials for identifying suitable lignocellulosic biomass when considering wood properties and indicate that potential genotypes for the production of biofuels are not necessarily the most productive.

Original languageEnglish
Pages (from-to)38-46
Number of pages9
JournalCanadian Journal of Forest Research
Volume42
Issue number1
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

enzymatic hydrolysis
Picea abies
clone
hydrolysis
reducing sugars
clones
decayed wood
wood properties
wood density
biofuels
Heterobasidion
Trichoderma viride
cellulases
heartwood
softwood
sapwood
glucans
sugar
karsts
particle size

Cite this

Rodriguez, Y.P. ; Puhakka-Tarvainen, H. ; Pastinen, O. ; Siika-aho, Matti ; Alvila, L. ; Turunen, O. ; Morales, L. ; Pappinen, A. / Susceptibility of pretreated wood sections of Norway spruce (Picea abies) clones to enzymatic hydrolysis. In: Canadian Journal of Forest Research. 2012 ; Vol. 42, No. 1. pp. 38-46.
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abstract = "The structure of softwoods, which confers resistance to degradation through hydrolysis and decay, currently limits their use for the production of biofuels. However, since wood is very heterogeneous, it is possible that differences in wood properties within and between trees could differentially affect its processability. In this research, heartwood (inner) and sapwood (outer) from Norway spruce (Picea abies (L.) Karst.) clones were enzymatically hydrolyzed by Trichoderma viride cellulases after concentrated acid pretreatment. Wood sections with two particle sizes were compared based on their susceptibility to enzymatic hydrolysis, evaluated by assaying the formation of hydrolysis products and measured as reducing sugar yield (RSY). We also studied the relationship between RSY and the susceptibility to Heterobasidion parviporum wood decay and whether these traits are reflected in wood density and yield. Wood from the outer section produced more RSY with higher glucan but lower lignin content than wood from the inner section. Furthermore, susceptibility to enzymatic hydrolysis was positively correlated with H. parviporum wood decay, while both processes were negatively correlated with wood density. Our results revealed the importance of clonal trials for identifying suitable lignocellulosic biomass when considering wood properties and indicate that potential genotypes for the production of biofuels are not necessarily the most productive.",
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Rodriguez, YP, Puhakka-Tarvainen, H, Pastinen, O, Siika-aho, M, Alvila, L, Turunen, O, Morales, L & Pappinen, A 2012, 'Susceptibility of pretreated wood sections of Norway spruce (Picea abies) clones to enzymatic hydrolysis', Canadian Journal of Forest Research, vol. 42, no. 1, pp. 38-46. https://doi.org/10.1139/X11-154

Susceptibility of pretreated wood sections of Norway spruce (Picea abies) clones to enzymatic hydrolysis. / Rodriguez, Y.P. (Corresponding Author); Puhakka-Tarvainen, H.; Pastinen, O.; Siika-aho, Matti; Alvila, L.; Turunen, O.; Morales, L.; Pappinen, A.

In: Canadian Journal of Forest Research, Vol. 42, No. 1, 2012, p. 38-46.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Susceptibility of pretreated wood sections of Norway spruce (Picea abies) clones to enzymatic hydrolysis

AU - Rodriguez, Y.P.

AU - Puhakka-Tarvainen, H.

AU - Pastinen, O.

AU - Siika-aho, Matti

AU - Alvila, L.

AU - Turunen, O.

AU - Morales, L.

AU - Pappinen, A.

PY - 2012

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N2 - The structure of softwoods, which confers resistance to degradation through hydrolysis and decay, currently limits their use for the production of biofuels. However, since wood is very heterogeneous, it is possible that differences in wood properties within and between trees could differentially affect its processability. In this research, heartwood (inner) and sapwood (outer) from Norway spruce (Picea abies (L.) Karst.) clones were enzymatically hydrolyzed by Trichoderma viride cellulases after concentrated acid pretreatment. Wood sections with two particle sizes were compared based on their susceptibility to enzymatic hydrolysis, evaluated by assaying the formation of hydrolysis products and measured as reducing sugar yield (RSY). We also studied the relationship between RSY and the susceptibility to Heterobasidion parviporum wood decay and whether these traits are reflected in wood density and yield. Wood from the outer section produced more RSY with higher glucan but lower lignin content than wood from the inner section. Furthermore, susceptibility to enzymatic hydrolysis was positively correlated with H. parviporum wood decay, while both processes were negatively correlated with wood density. Our results revealed the importance of clonal trials for identifying suitable lignocellulosic biomass when considering wood properties and indicate that potential genotypes for the production of biofuels are not necessarily the most productive.

AB - The structure of softwoods, which confers resistance to degradation through hydrolysis and decay, currently limits their use for the production of biofuels. However, since wood is very heterogeneous, it is possible that differences in wood properties within and between trees could differentially affect its processability. In this research, heartwood (inner) and sapwood (outer) from Norway spruce (Picea abies (L.) Karst.) clones were enzymatically hydrolyzed by Trichoderma viride cellulases after concentrated acid pretreatment. Wood sections with two particle sizes were compared based on their susceptibility to enzymatic hydrolysis, evaluated by assaying the formation of hydrolysis products and measured as reducing sugar yield (RSY). We also studied the relationship between RSY and the susceptibility to Heterobasidion parviporum wood decay and whether these traits are reflected in wood density and yield. Wood from the outer section produced more RSY with higher glucan but lower lignin content than wood from the inner section. Furthermore, susceptibility to enzymatic hydrolysis was positively correlated with H. parviporum wood decay, while both processes were negatively correlated with wood density. Our results revealed the importance of clonal trials for identifying suitable lignocellulosic biomass when considering wood properties and indicate that potential genotypes for the production of biofuels are not necessarily the most productive.

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DO - 10.1139/X11-154

M3 - Article

VL - 42

SP - 38

EP - 46

JO - Canadian Journal of Forest Research

JF - Canadian Journal of Forest Research

SN - 0045-5067

IS - 1

ER -