Sustained oscillations in free-energy state and hexose phosphates in yeast

Peter Richard, Bas Teusink, Mirte B. Hemker, Karel Van Dam, Hans V. Westerhoff

Research output: Contribution to journalArticleScientificpeer-review

87 Citations (Scopus)

Abstract

In a population of intact cells of the yeast Saccharomyces cerevisiae the dynamics of glycolytic metabolism were investigated under the condition of sustained oscillations. At 5-s intervals cells were quenched in -40°C methanol, extracted and the intracellular concentrations of glycolytic metabolites, adenine nucleotides and phosphate were analysed. Oscillations were found for the glycolytic intermediates glucose 6-phosphate, fructose 6- phosphate and fructose 1,6-bisphosphate. At variance with earlier reports on transient glycolytic oscillations, some intermediates further down the glycolytic pathway did not oscillate significantly, even though NADH did. In addition, the adenylate energy charge and the free energy of ATP hydrolysis oscillated significantly. Dynamic coupling through the latter may be responsible for this effective compartmentation of glycolytic dynamics.

Original languageEnglish
Pages (from-to)731-740
Number of pages10
JournalYeast
Volume12
Issue number8
DOIs
Publication statusPublished - 30 Jun 1996
MoE publication typeNot Eligible

Fingerprint

Hexoses
hexoses
Yeast
Electron energy levels
Free energy
oscillation
Fructose
Phosphates
Yeasts
phosphates
yeasts
Glucose-6-Phosphate
Adenine Nucleotides
energy
NAD
Methanol
Saccharomyces cerevisiae
fructose 6-phosphate
glucose 6-phosphate
Hydrolysis

Keywords

  • glycolysis
  • intracellular metabolites
  • oscillation
  • Saccharomyces cerevisiae
  • yeast

Cite this

Richard, Peter ; Teusink, Bas ; Hemker, Mirte B. ; Van Dam, Karel ; Westerhoff, Hans V. / Sustained oscillations in free-energy state and hexose phosphates in yeast. In: Yeast. 1996 ; Vol. 12, No. 8. pp. 731-740.
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Sustained oscillations in free-energy state and hexose phosphates in yeast. / Richard, Peter; Teusink, Bas; Hemker, Mirte B.; Van Dam, Karel; Westerhoff, Hans V.

In: Yeast, Vol. 12, No. 8, 30.06.1996, p. 731-740.

Research output: Contribution to journalArticleScientificpeer-review

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