Synchronized heat flux oscillations in yeast cell populations

Bas Teusink, Christer Larsson, Jasper Diderich, Peter Richard, Karel Van Dam, Lena Gustafsson, Hans V. Westerhoff

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

Microcalorimetry was adapted to the study of glycolytic oscillations in suspensions of intact yeast cells. A correction procedure was developed for the distortion of the amplitude and phase of the heat signal, caused by the slow response of the calorimeter. This made it possible to observe oscillations in the heat production rate with a period of less than 1 min, and a relative amplitude of 5-10%. By simultaneously measuring the heat flux and concentrations of glycolytic metabolites, and by comparing acetaldehyde- induced phase shifts of the heat flux oscillations with those of NADH oscillations, the heat flux was found to be 100° out of phase with glucose 6-phosphate, 80° out of phase with fructose 1,6-bisphosphate, and in phase with NADH. The flux measurement made possible by microcalorimetry allowed the recognition of (i) changes in metabolic capacity that may affect glycolytic dynamics, (ii) implications of glucose carrier kinetics for glycolytic dynamics and (iii) the continued requirement for an acetaldehyde trapping agent for the oscillations.

Original languageEnglish
Pages (from-to)24442-24448
Number of pages7
JournalJournal of Biological Chemistry
Volume271
Issue number40
DOIs
Publication statusPublished - 11 Oct 1996
MoE publication typeNot Eligible

Fingerprint

Yeast
Heat flux
Acetaldehyde
Hot Temperature
Yeasts
Cells
NAD
Population
Glucose-6-Phosphate
Metabolites
Calorimeters
Phase shift
Thermogenesis
Suspensions
Fluxes
Glucose
Kinetics
fructose-1,6-diphosphate

Cite this

Teusink, B., Larsson, C., Diderich, J., Richard, P., Van Dam, K., Gustafsson, L., & Westerhoff, H. V. (1996). Synchronized heat flux oscillations in yeast cell populations. Journal of Biological Chemistry, 271(40), 24442-24448. https://doi.org/10.1074/jbc.271.40.24442
Teusink, Bas ; Larsson, Christer ; Diderich, Jasper ; Richard, Peter ; Van Dam, Karel ; Gustafsson, Lena ; Westerhoff, Hans V. / Synchronized heat flux oscillations in yeast cell populations. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 40. pp. 24442-24448.
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Teusink, B, Larsson, C, Diderich, J, Richard, P, Van Dam, K, Gustafsson, L & Westerhoff, HV 1996, 'Synchronized heat flux oscillations in yeast cell populations', Journal of Biological Chemistry, vol. 271, no. 40, pp. 24442-24448. https://doi.org/10.1074/jbc.271.40.24442

Synchronized heat flux oscillations in yeast cell populations. / Teusink, Bas; Larsson, Christer; Diderich, Jasper; Richard, Peter; Van Dam, Karel; Gustafsson, Lena; Westerhoff, Hans V.

In: Journal of Biological Chemistry, Vol. 271, No. 40, 11.10.1996, p. 24442-24448.

Research output: Contribution to journalArticleScientificpeer-review

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Teusink B, Larsson C, Diderich J, Richard P, Van Dam K, Gustafsson L et al. Synchronized heat flux oscillations in yeast cell populations. Journal of Biological Chemistry. 1996 Oct 11;271(40):24442-24448. https://doi.org/10.1074/jbc.271.40.24442