Synchronized heat flux oscillations in yeast cell populations

Bas Teusink, Christer Larsson, Jasper Diderich, Peter Richard, Karel Van Dam, Lena Gustafsson, Hans V. Westerhoff

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

Microcalorimetry was adapted to the study of glycolytic oscillations in suspensions of intact yeast cells. A correction procedure was developed for the distortion of the amplitude and phase of the heat signal, caused by the slow response of the calorimeter. This made it possible to observe oscillations in the heat production rate with a period of less than 1 min, and a relative amplitude of 5-10%. By simultaneously measuring the heat flux and concentrations of glycolytic metabolites, and by comparing acetaldehyde- induced phase shifts of the heat flux oscillations with those of NADH oscillations, the heat flux was found to be 100° out of phase with glucose 6-phosphate, 80° out of phase with fructose 1,6-bisphosphate, and in phase with NADH. The flux measurement made possible by microcalorimetry allowed the recognition of (i) changes in metabolic capacity that may affect glycolytic dynamics, (ii) implications of glucose carrier kinetics for glycolytic dynamics and (iii) the continued requirement for an acetaldehyde trapping agent for the oscillations.

Original languageEnglish
Pages (from-to)24442-24448
Number of pages7
JournalJournal of Biological Chemistry
Volume271
Issue number40
DOIs
Publication statusPublished - 11 Oct 1996
MoE publication typeNot Eligible

Fingerprint Dive into the research topics of 'Synchronized heat flux oscillations in yeast cell populations'. Together they form a unique fingerprint.

  • Cite this

    Teusink, B., Larsson, C., Diderich, J., Richard, P., Van Dam, K., Gustafsson, L., & Westerhoff, H. V. (1996). Synchronized heat flux oscillations in yeast cell populations. Journal of Biological Chemistry, 271(40), 24442-24448. https://doi.org/10.1074/jbc.271.40.24442