Syndecan-1 supports integrin a2ß1-mediated adhesion to collagen

Karoliina Vuoriluoto, Johanna Jokinen, Katja Kallio, Markku Salmivirta, Jyrki Heino, Johanna Ivaska (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

49 Citations (Scopus)

Abstract

Several different receptor molecules act in concert to regulate cell adhesion. Among these are cell-surface proteoglycans and integrins, which collaborate extensively in mediating binding of cells to extracellular matrix molecules fibronectin and vitronectin. However, very little is known about possible functional synergism between proteoglycans and integrins during adhesion of cells to collagen, although collagen is the most abundant protein in the human body. Here we show that cell-surface heparan sulphate proteoglycans (HSPGs) support integrin α2β1-mediated adhesion to collagen. Cells made devoid of HSPGs either by genetic means or by enzymatic digestions were unable to adhere to collagen via α2β1 integrin. HSPG-deficient cells also displayed impaired spreading and actin organization on collagen. Among different HSPG molecules syndecan-1 was found to play an important role in supporting α2β1 integrin-mediated adhesion. Using overexpression and knock-down experiments we demonstrated that syndecan-1, but not syndecan-2 or -4, enhanced binding of α2β1 to collagen. Moreover, syndecan-1 co-localized with α2β1 integrin and contributed to proper organization of cortical actin. Finally, crosstalk between syndecan-1 and α2β1 integrin was found to enhance the transcription of matrix metalloproteinase-1 in response to collagen binding. Our findings thus suggest that a previously unknown link between integrin α2β1 and syndecan-1 is important in regulating cell adhesion to collagen and in triggering integrin downstream signalling.
Original languageEnglish
Pages (from-to)3369-3381
Number of pages13
JournalExperimental Cell Research
Volume314
Issue number18
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Syndecan-1
Integrins
Collagen
Heparan Sulfate Proteoglycans
Cell Adhesion
Proteoglycans
Actins
Syndecan-2
Vitronectin
Matrix Metalloproteinase 1
Human Body
Fibronectins
Extracellular Matrix
Digestion

Keywords

  • Cell adhesion
  • Integrin
  • Syndecan
  • Collagen

Cite this

Vuoriluoto, K., Jokinen, J., Kallio, K., Salmivirta, M., Heino, J., & Ivaska, J. (2008). Syndecan-1 supports integrin a2ß1-mediated adhesion to collagen. Experimental Cell Research, 314(18), 3369-3381. https://doi.org/10.1016/j.yexcr.2008.07.005
Vuoriluoto, Karoliina ; Jokinen, Johanna ; Kallio, Katja ; Salmivirta, Markku ; Heino, Jyrki ; Ivaska, Johanna. / Syndecan-1 supports integrin a2ß1-mediated adhesion to collagen. In: Experimental Cell Research. 2008 ; Vol. 314, No. 18. pp. 3369-3381.
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Vuoriluoto, K, Jokinen, J, Kallio, K, Salmivirta, M, Heino, J & Ivaska, J 2008, 'Syndecan-1 supports integrin a2ß1-mediated adhesion to collagen', Experimental Cell Research, vol. 314, no. 18, pp. 3369-3381. https://doi.org/10.1016/j.yexcr.2008.07.005

Syndecan-1 supports integrin a2ß1-mediated adhesion to collagen. / Vuoriluoto, Karoliina; Jokinen, Johanna; Kallio, Katja; Salmivirta, Markku; Heino, Jyrki; Ivaska, Johanna (Corresponding Author).

In: Experimental Cell Research, Vol. 314, No. 18, 2008, p. 3369-3381.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Syndecan-1 supports integrin a2ß1-mediated adhesion to collagen

AU - Vuoriluoto, Karoliina

AU - Jokinen, Johanna

AU - Kallio, Katja

AU - Salmivirta, Markku

AU - Heino, Jyrki

AU - Ivaska, Johanna

PY - 2008

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AB - Several different receptor molecules act in concert to regulate cell adhesion. Among these are cell-surface proteoglycans and integrins, which collaborate extensively in mediating binding of cells to extracellular matrix molecules fibronectin and vitronectin. However, very little is known about possible functional synergism between proteoglycans and integrins during adhesion of cells to collagen, although collagen is the most abundant protein in the human body. Here we show that cell-surface heparan sulphate proteoglycans (HSPGs) support integrin α2β1-mediated adhesion to collagen. Cells made devoid of HSPGs either by genetic means or by enzymatic digestions were unable to adhere to collagen via α2β1 integrin. HSPG-deficient cells also displayed impaired spreading and actin organization on collagen. Among different HSPG molecules syndecan-1 was found to play an important role in supporting α2β1 integrin-mediated adhesion. Using overexpression and knock-down experiments we demonstrated that syndecan-1, but not syndecan-2 or -4, enhanced binding of α2β1 to collagen. Moreover, syndecan-1 co-localized with α2β1 integrin and contributed to proper organization of cortical actin. Finally, crosstalk between syndecan-1 and α2β1 integrin was found to enhance the transcription of matrix metalloproteinase-1 in response to collagen binding. Our findings thus suggest that a previously unknown link between integrin α2β1 and syndecan-1 is important in regulating cell adhesion to collagen and in triggering integrin downstream signalling.

KW - Cell adhesion

KW - Integrin

KW - Syndecan

KW - Collagen

U2 - 10.1016/j.yexcr.2008.07.005

DO - 10.1016/j.yexcr.2008.07.005

M3 - Article

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EP - 3381

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

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ER -

Vuoriluoto K, Jokinen J, Kallio K, Salmivirta M, Heino J, Ivaska J. Syndecan-1 supports integrin a2ß1-mediated adhesion to collagen. Experimental Cell Research. 2008;314(18):3369-3381. https://doi.org/10.1016/j.yexcr.2008.07.005