Synergistic action of xylanase and mannanase improves the total hydrolysis of softwood

Aniko Várnai (Corresponding Author), L. Huikko, Jaakko Pere, Matti Siika-aho, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

94 Citations (Scopus)


The impact of xylan and glucomannan hydrolysis on cellulose hydrolysis was studied on five pretreated softwood substrates with different xylan and glucomannan contents, both varying from 0.2% to 6.9%, using mixtures of purified enzymes. The supplementation of pure cellulase mixture with non-specific endoglucanase TrCel7B and xylanase TrXyn11 enhanced the hydrolysis of all substrates, except the steam pretreated spruce, by more than 50%. The addition of endo-β-mannanase increased the overall hydrolysis yield by 20–25%, liberating significantly more glucose than theoretically present in glucomannan. When supplemented together, xylanolytic and mannanolytic enzymes acted synergistically with cellulases. Moreover, a linear correlation was observed between the hydrolysis of polysaccharides, irrespective of the composition, indicating that glucomannan and xylan form a complex network of polysaccharides around the cellulosic fibres extending throughout the lignocellulosic matrix. Both hemicellulolytic enzymes are crucial as accessory enzymes when designing efficient mixtures for the total hydrolysis of lignocellulosic substrates containing both hemicelluloses.

Original languageEnglish
Pages (from-to)9096-9104
Number of pages9
JournalBioresource Technology
Issue number19
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed


  • Enzymatic hydrolysis
  • lignocellulose
  • mannanase
  • synergism
  • xylanase

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