Synthesis and Langmuir Film Formation of N-(e- Maleimidoca-proyl)(dilinoleoylphosphatidyl)ethanolamine

Tapani Viitala, Martin Albers, Inger Vikholm, Jouko Peltonen

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

N-(ε-Maleimidocaproyl)(dilinoleoylphosphatidyl)ethanolamine (DLiPE-EMC) has been synthesized, representing a new polymerizable lipid with a linking group for covalent attachment of proteins for immunosensor applications.
The linker lipid could be prepared by performing both the synthesis and separation steps under argon and avoiding heating. The product was characterized by 600 MHz 1H NMR, mass, and UV−vis spectrometry.
Monomolecular films were successfully prepared on a Langmuir trough, and the characteristic surface pressure−area curves were measured on different subphases. A condensation of the monolayer could be achieved by introducing a micromolar concentration of uranyl ions in the subphase.
Surface pressure−area isotherms of pure and mixed DLiPE/DLiPE-EMC monolayers were also measured. UV irradiation experiments were performed to demonstrate the polymerizability of the mixed monolayers.
Original languageEnglish
Pages (from-to)1272-1277
JournalLangmuir
Volume14
Issue number5
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

Fingerprint

Ethanolamines
Ethanolamine
Langmuir Blodgett films
lipids
Monolayers
monomolecular films
Electromagnetic compatibility
synthesis
troughs
Lipids
spectroscopy
attachment
isotherms
mass spectroscopy
condensation
argon
proteins
Immunosensors
nuclear magnetic resonance
irradiation

Cite this

Viitala, Tapani ; Albers, Martin ; Vikholm, Inger ; Peltonen, Jouko. / Synthesis and Langmuir Film Formation of N-(e- Maleimidoca-proyl)(dilinoleoylphosphatidyl)ethanolamine. In: Langmuir. 1998 ; Vol. 14, No. 5. pp. 1272-1277.
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title = "Synthesis and Langmuir Film Formation of N-(e- Maleimidoca-proyl)(dilinoleoylphosphatidyl)ethanolamine",
abstract = "N-(ε-Maleimidocaproyl)(dilinoleoylphosphatidyl)ethanolamine (DLiPE-EMC) has been synthesized, representing a new polymerizable lipid with a linking group for covalent attachment of proteins for immunosensor applications. The linker lipid could be prepared by performing both the synthesis and separation steps under argon and avoiding heating. The product was characterized by 600 MHz 1H NMR, mass, and UV−vis spectrometry. Monomolecular films were successfully prepared on a Langmuir trough, and the characteristic surface pressure−area curves were measured on different subphases. A condensation of the monolayer could be achieved by introducing a micromolar concentration of uranyl ions in the subphase. Surface pressure−area isotherms of pure and mixed DLiPE/DLiPE-EMC monolayers were also measured. UV irradiation experiments were performed to demonstrate the polymerizability of the mixed monolayers.",
author = "Tapani Viitala and Martin Albers and Inger Vikholm and Jouko Peltonen",
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journal = "Langmuir",
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Synthesis and Langmuir Film Formation of N-(e- Maleimidoca-proyl)(dilinoleoylphosphatidyl)ethanolamine. / Viitala, Tapani; Albers, Martin; Vikholm, Inger; Peltonen, Jouko.

In: Langmuir, Vol. 14, No. 5, 1998, p. 1272-1277.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Synthesis and Langmuir Film Formation of N-(e- Maleimidoca-proyl)(dilinoleoylphosphatidyl)ethanolamine

AU - Viitala, Tapani

AU - Albers, Martin

AU - Vikholm, Inger

AU - Peltonen, Jouko

N1 - Project code: K8SU00019

PY - 1998

Y1 - 1998

N2 - N-(ε-Maleimidocaproyl)(dilinoleoylphosphatidyl)ethanolamine (DLiPE-EMC) has been synthesized, representing a new polymerizable lipid with a linking group for covalent attachment of proteins for immunosensor applications. The linker lipid could be prepared by performing both the synthesis and separation steps under argon and avoiding heating. The product was characterized by 600 MHz 1H NMR, mass, and UV−vis spectrometry. Monomolecular films were successfully prepared on a Langmuir trough, and the characteristic surface pressure−area curves were measured on different subphases. A condensation of the monolayer could be achieved by introducing a micromolar concentration of uranyl ions in the subphase. Surface pressure−area isotherms of pure and mixed DLiPE/DLiPE-EMC monolayers were also measured. UV irradiation experiments were performed to demonstrate the polymerizability of the mixed monolayers.

AB - N-(ε-Maleimidocaproyl)(dilinoleoylphosphatidyl)ethanolamine (DLiPE-EMC) has been synthesized, representing a new polymerizable lipid with a linking group for covalent attachment of proteins for immunosensor applications. The linker lipid could be prepared by performing both the synthesis and separation steps under argon and avoiding heating. The product was characterized by 600 MHz 1H NMR, mass, and UV−vis spectrometry. Monomolecular films were successfully prepared on a Langmuir trough, and the characteristic surface pressure−area curves were measured on different subphases. A condensation of the monolayer could be achieved by introducing a micromolar concentration of uranyl ions in the subphase. Surface pressure−area isotherms of pure and mixed DLiPE/DLiPE-EMC monolayers were also measured. UV irradiation experiments were performed to demonstrate the polymerizability of the mixed monolayers.

U2 - 10.1021/la971075s

DO - 10.1021/la971075s

M3 - Article

VL - 14

SP - 1272

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JO - Langmuir

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