Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines: Large scale production of the E1 protein

Tove Johansson, Annalena Enestam, Robert Kronqvist, Michel Schmidt, Nina Tuominen, Stefan Weiss, Christian Oker-Blom (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

The two envelope glycoproteins of rubella virus (RV), El of 58 kDa and E2 of 42–47 kDa, were individually expressed in lepidopteran Spodoptera frugiperda as well as in Trichoplusia ni insect cells using baculovirus vectors. The authentic signal sequences of E1 and E2 were replaced with the honeybee melittin signal sequence, allowing efficient entrance into the secretory pathway of the insect cell. In addition, the hydrophobic transmembrane anchors at the carboxyl termini of E1 and E2 proteins were removed to enable secretion rather than maintenance in the cellular membranes. Synthesis of the recombinant proteins in the absence and presence of tunicamycin revealed that both E1 and E2 were glycosylated with apparent molecular weights of 52 kDa and 37 kDa, respectively. Recombinant E2 appeared to be partially secreted, whereas E1 was essentially found inside the infected insect cell. The E1 protein was produced in large scale using a 10−1 bioreactor and serum-free medium (SFM). Purification of the recombinant protein product was performed from cytoplasmic extracts by ammonium sulphate precipitation followed by Concanavalin A affinity chromatography. This type of purified recombinant viral glycoproteins may be useful not only in diagnostic medicine or for immunization, but should enable studies designed to solve the structure of the virus particle.
Original languageEnglish
Pages (from-to)171-180
Number of pages11
JournalJournal of Biotechnology
Volume50
Issue number2-3
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

Fingerprint

Rubella virus
Recombinant proteins
Glycoproteins
Protein Sorting Signals
Viruses
Recombinant Proteins
Insects
Cells
Melitten
Immunization
Proteins
Affinity chromatography
Tunicamycin
Cell Line
Serum-Free Culture Media
Ammonium Sulfate
Concanavalin A
Bioreactors
Anchors
Medicine

Cite this

Johansson, Tove ; Enestam, Annalena ; Kronqvist, Robert ; Schmidt, Michel ; Tuominen, Nina ; Weiss, Stefan ; Oker-Blom, Christian. / Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines : Large scale production of the E1 protein. In: Journal of Biotechnology. 1996 ; Vol. 50, No. 2-3. pp. 171-180.
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title = "Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines: Large scale production of the E1 protein",
abstract = "The two envelope glycoproteins of rubella virus (RV), El of 58 kDa and E2 of 42–47 kDa, were individually expressed in lepidopteran Spodoptera frugiperda as well as in Trichoplusia ni insect cells using baculovirus vectors. The authentic signal sequences of E1 and E2 were replaced with the honeybee melittin signal sequence, allowing efficient entrance into the secretory pathway of the insect cell. In addition, the hydrophobic transmembrane anchors at the carboxyl termini of E1 and E2 proteins were removed to enable secretion rather than maintenance in the cellular membranes. Synthesis of the recombinant proteins in the absence and presence of tunicamycin revealed that both E1 and E2 were glycosylated with apparent molecular weights of 52 kDa and 37 kDa, respectively. Recombinant E2 appeared to be partially secreted, whereas E1 was essentially found inside the infected insect cell. The E1 protein was produced in large scale using a 10−1 bioreactor and serum-free medium (SFM). Purification of the recombinant protein product was performed from cytoplasmic extracts by ammonium sulphate precipitation followed by Concanavalin A affinity chromatography. This type of purified recombinant viral glycoproteins may be useful not only in diagnostic medicine or for immunization, but should enable studies designed to solve the structure of the virus particle.",
author = "Tove Johansson and Annalena Enestam and Robert Kronqvist and Michel Schmidt and Nina Tuominen and Stefan Weiss and Christian Oker-Blom",
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Johansson, T, Enestam, A, Kronqvist, R, Schmidt, M, Tuominen, N, Weiss, S & Oker-Blom, C 1996, 'Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines: Large scale production of the E1 protein', Journal of Biotechnology, vol. 50, no. 2-3, pp. 171-180. https://doi.org/10.1016/0168-1656(96)01562-3

Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines : Large scale production of the E1 protein. / Johansson, Tove; Enestam, Annalena; Kronqvist, Robert; Schmidt, Michel; Tuominen, Nina; Weiss, Stefan; Oker-Blom, Christian (Corresponding Author).

In: Journal of Biotechnology, Vol. 50, No. 2-3, 1996, p. 171-180.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Synthesis of soluble rubella virus spike proteins in two lepidopteran insect cell lines

T2 - Large scale production of the E1 protein

AU - Johansson, Tove

AU - Enestam, Annalena

AU - Kronqvist, Robert

AU - Schmidt, Michel

AU - Tuominen, Nina

AU - Weiss, Stefan

AU - Oker-Blom, Christian

N1 - Project code: B5SU00171

PY - 1996

Y1 - 1996

N2 - The two envelope glycoproteins of rubella virus (RV), El of 58 kDa and E2 of 42–47 kDa, were individually expressed in lepidopteran Spodoptera frugiperda as well as in Trichoplusia ni insect cells using baculovirus vectors. The authentic signal sequences of E1 and E2 were replaced with the honeybee melittin signal sequence, allowing efficient entrance into the secretory pathway of the insect cell. In addition, the hydrophobic transmembrane anchors at the carboxyl termini of E1 and E2 proteins were removed to enable secretion rather than maintenance in the cellular membranes. Synthesis of the recombinant proteins in the absence and presence of tunicamycin revealed that both E1 and E2 were glycosylated with apparent molecular weights of 52 kDa and 37 kDa, respectively. Recombinant E2 appeared to be partially secreted, whereas E1 was essentially found inside the infected insect cell. The E1 protein was produced in large scale using a 10−1 bioreactor and serum-free medium (SFM). Purification of the recombinant protein product was performed from cytoplasmic extracts by ammonium sulphate precipitation followed by Concanavalin A affinity chromatography. This type of purified recombinant viral glycoproteins may be useful not only in diagnostic medicine or for immunization, but should enable studies designed to solve the structure of the virus particle.

AB - The two envelope glycoproteins of rubella virus (RV), El of 58 kDa and E2 of 42–47 kDa, were individually expressed in lepidopteran Spodoptera frugiperda as well as in Trichoplusia ni insect cells using baculovirus vectors. The authentic signal sequences of E1 and E2 were replaced with the honeybee melittin signal sequence, allowing efficient entrance into the secretory pathway of the insect cell. In addition, the hydrophobic transmembrane anchors at the carboxyl termini of E1 and E2 proteins were removed to enable secretion rather than maintenance in the cellular membranes. Synthesis of the recombinant proteins in the absence and presence of tunicamycin revealed that both E1 and E2 were glycosylated with apparent molecular weights of 52 kDa and 37 kDa, respectively. Recombinant E2 appeared to be partially secreted, whereas E1 was essentially found inside the infected insect cell. The E1 protein was produced in large scale using a 10−1 bioreactor and serum-free medium (SFM). Purification of the recombinant protein product was performed from cytoplasmic extracts by ammonium sulphate precipitation followed by Concanavalin A affinity chromatography. This type of purified recombinant viral glycoproteins may be useful not only in diagnostic medicine or for immunization, but should enable studies designed to solve the structure of the virus particle.

U2 - 10.1016/0168-1656(96)01562-3

DO - 10.1016/0168-1656(96)01562-3

M3 - Article

VL - 50

SP - 171

EP - 180

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 2-3

ER -