Tandem mass spectrometry for on-line fermentatiion monitoring

M. Hayward, A. Lister, G. Tsao, Tapio Kotiaho, R. Narayan, R. Cooks, G. Austin

Research output: Contribution to journalArticleScientific

17 Citations (Scopus)

Abstract

Membrane introduction mass spectrometry has been employed for on-line determination of the major products and volatile metabolites ofBacillus polymyxa fermentation. Samples were introduced into the mass spectrometer via a direct insertion membrane probe in which the aqueous solution flowed past a membrane located in the ion source of the mass spectrometer.
Concentrations of the products 2,3-butanediol, acetoin, ethanol and acetic acid in fermentation broth were measured by tandem mass spectrometry after permeation through the membrane and ionization by chemical ionization. External standards were employed for quantification and a large linear response range was available for each of the major products observed. Dissolved CO2 and O2, as well as CO2 in the off gases, were also monitored on-line by mass spectrometry.
The use of tandem mass spectrometry has allowed the identification of products that were not previously known to be present in measurable amounts.
Original languageEnglish
Pages (from-to)361-364
JournalBiotechnology Techniques
Volume3
Issue number6
DOIs
Publication statusPublished - 1989
MoE publication typeB1 Article in a scientific magazine

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Tandem Mass Spectrometry
Mass spectrometry
Membranes
Monitoring
Mass spectrometers
Fermentation
Ionization
Mass Spectrometry
Plasmodiophorida
Acetoin
Ion sources
Metabolites
Permeation
Acetic Acid
Ethanol
Gases
Ions

Cite this

Hayward, M., Lister, A., Tsao, G., Kotiaho, T., Narayan, R., Cooks, R., & Austin, G. (1989). Tandem mass spectrometry for on-line fermentatiion monitoring. Biotechnology Techniques, 3(6), 361-364. https://doi.org/10.1007/BF01874999
Hayward, M. ; Lister, A. ; Tsao, G. ; Kotiaho, Tapio ; Narayan, R. ; Cooks, R. ; Austin, G. / Tandem mass spectrometry for on-line fermentatiion monitoring. In: Biotechnology Techniques. 1989 ; Vol. 3, No. 6. pp. 361-364.
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Hayward, M, Lister, A, Tsao, G, Kotiaho, T, Narayan, R, Cooks, R & Austin, G 1989, 'Tandem mass spectrometry for on-line fermentatiion monitoring', Biotechnology Techniques, vol. 3, no. 6, pp. 361-364. https://doi.org/10.1007/BF01874999

Tandem mass spectrometry for on-line fermentatiion monitoring. / Hayward, M.; Lister, A.; Tsao, G.; Kotiaho, Tapio; Narayan, R.; Cooks, R.; Austin, G.

In: Biotechnology Techniques, Vol. 3, No. 6, 1989, p. 361-364.

Research output: Contribution to journalArticleScientific

TY - JOUR

T1 - Tandem mass spectrometry for on-line fermentatiion monitoring

AU - Hayward, M.

AU - Lister, A.

AU - Tsao, G.

AU - Kotiaho, Tapio

AU - Narayan, R.

AU - Cooks, R.

AU - Austin, G.

PY - 1989

Y1 - 1989

N2 - Membrane introduction mass spectrometry has been employed for on-line determination of the major products and volatile metabolites ofBacillus polymyxa fermentation. Samples were introduced into the mass spectrometer via a direct insertion membrane probe in which the aqueous solution flowed past a membrane located in the ion source of the mass spectrometer. Concentrations of the products 2,3-butanediol, acetoin, ethanol and acetic acid in fermentation broth were measured by tandem mass spectrometry after permeation through the membrane and ionization by chemical ionization. External standards were employed for quantification and a large linear response range was available for each of the major products observed. Dissolved CO2 and O2, as well as CO2 in the off gases, were also monitored on-line by mass spectrometry.The use of tandem mass spectrometry has allowed the identification of products that were not previously known to be present in measurable amounts.

AB - Membrane introduction mass spectrometry has been employed for on-line determination of the major products and volatile metabolites ofBacillus polymyxa fermentation. Samples were introduced into the mass spectrometer via a direct insertion membrane probe in which the aqueous solution flowed past a membrane located in the ion source of the mass spectrometer. Concentrations of the products 2,3-butanediol, acetoin, ethanol and acetic acid in fermentation broth were measured by tandem mass spectrometry after permeation through the membrane and ionization by chemical ionization. External standards were employed for quantification and a large linear response range was available for each of the major products observed. Dissolved CO2 and O2, as well as CO2 in the off gases, were also monitored on-line by mass spectrometry.The use of tandem mass spectrometry has allowed the identification of products that were not previously known to be present in measurable amounts.

U2 - 10.1007/BF01874999

DO - 10.1007/BF01874999

M3 - Article

VL - 3

SP - 361

EP - 364

JO - Biotechnology Techniques

JF - Biotechnology Techniques

SN - 0951-208X

IS - 6

ER -

Hayward M, Lister A, Tsao G, Kotiaho T, Narayan R, Cooks R et al. Tandem mass spectrometry for on-line fermentatiion monitoring. Biotechnology Techniques. 1989;3(6):361-364. https://doi.org/10.1007/BF01874999