Abstract
Membrane introduction mass spectrometry has been employed for on-line determination of the major products and volatile metabolites ofBacillus polymyxa fermentation. Samples were introduced into the mass spectrometer via a direct insertion membrane probe in which the aqueous solution flowed past a membrane located in the ion source of the mass spectrometer.
Concentrations of the products 2,3-butanediol, acetoin, ethanol and acetic acid in fermentation broth were measured by tandem mass spectrometry after permeation through the membrane and ionization by chemical ionization. External standards were employed for quantification and a large linear response range was available for each of the major products observed. Dissolved CO2 and O2, as well as CO2 in the off gases, were also monitored on-line by mass spectrometry.
The use of tandem mass spectrometry has allowed the identification of products that were not previously known to be present in measurable amounts.
Concentrations of the products 2,3-butanediol, acetoin, ethanol and acetic acid in fermentation broth were measured by tandem mass spectrometry after permeation through the membrane and ionization by chemical ionization. External standards were employed for quantification and a large linear response range was available for each of the major products observed. Dissolved CO2 and O2, as well as CO2 in the off gases, were also monitored on-line by mass spectrometry.
The use of tandem mass spectrometry has allowed the identification of products that were not previously known to be present in measurable amounts.
| Original language | English |
|---|---|
| Pages (from-to) | 361-364 |
| Journal | Biotechnology Techniques |
| Volume | 3 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1989 |
| MoE publication type | B1 Article in a scientific magazine |