Abstract
Pectin, a cheap and abundant raw material has a huge potential in white biotechnology. D-galacturonate, the main component of pectin, is catabolised
to pyruvate and glycerol through a reductive pathway in eukaryotic microorganisms like filamentous fungi. This pathway also enables the utilization of pectin for the production of more valuable compounds. An example from that is the production of meso-galactarate (mucic acid) in metabolically
engineered fungal strains. Enzymes functioning on the reductive D-galacturonate pathway are a D-galacturonate reductase, L-galactonate dehydratase, 2-keto-3-deoxy-galactonate aldolase and glyceraldehyde reductase. The genes coding for these enzymes in Aspergillus niger are gaaA, gaaB, gaaC and gaaD, respectively, while in Hypocrea jecorina (Trichoderma reesei) the corresponding genes are gar1, lgd1, lga1 and gld1. A. niger also has a homologue gene sequence for H. jecorina gar1 whereas H. jecorina has a homologue gene sequence for A. niger gaaA respectively. Functions of these
two additional D-galacturonate reductase genes have remained unclear. We have now studied the roles of these two genes.
to pyruvate and glycerol through a reductive pathway in eukaryotic microorganisms like filamentous fungi. This pathway also enables the utilization of pectin for the production of more valuable compounds. An example from that is the production of meso-galactarate (mucic acid) in metabolically
engineered fungal strains. Enzymes functioning on the reductive D-galacturonate pathway are a D-galacturonate reductase, L-galactonate dehydratase, 2-keto-3-deoxy-galactonate aldolase and glyceraldehyde reductase. The genes coding for these enzymes in Aspergillus niger are gaaA, gaaB, gaaC and gaaD, respectively, while in Hypocrea jecorina (Trichoderma reesei) the corresponding genes are gar1, lgd1, lga1 and gld1. A. niger also has a homologue gene sequence for H. jecorina gar1 whereas H. jecorina has a homologue gene sequence for A. niger gaaA respectively. Functions of these
two additional D-galacturonate reductase genes have remained unclear. We have now studied the roles of these two genes.
| Original language | English |
|---|---|
| Title of host publication | 26th Fungal Genetics Conference at Asilomar March 15-20, 2011 |
| Publisher | Genetics Society of America |
| Chapter | Poster Abstracts |
| Pages | 205 |
| Publication status | Published - 2011 |
| MoE publication type | Not Eligible |
| Event | 26th Fungal Genetics Conference - Asilomar, United States Duration: 15 Mar 2011 → 20 Mar 2011 |
Publication series
| Series | Fungal Genetics Reports |
|---|---|
| Number | Supplement |
| Volume | 58 |
Conference
| Conference | 26th Fungal Genetics Conference |
|---|---|
| Country/Territory | United States |
| City | Asilomar |
| Period | 15/03/11 → 20/03/11 |