Abstract
The antimicrobial effects of the wood-associated polyphenolic compounds
pinosylvin, pinosylvin monomethyl ether, astringin, piceatannol,
isorhapontin, isorhapontigenin, cycloXMe, dHIMP, ArX, and ArXOH were
assessed against both Gram-negative (Salmonella) and Gram-positive bacteria (Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus) and yeasts (Candida tropicalis, Saccharomyces cerevisiae).
Particularly the stilbenes pinosylvin, its monomethyl ether and
piceatannol demonstrated a clear antimicrobial activity, which in the
case of pinosylvin was present also in food matrices like sauerkraut,
gravlax and berry jam, but not in milk. The destabilization of the outer
membrane of Gram-negative microorganisms, as well as interactions with
the cell membrane, as indicated by the NPN uptake and LIVE/DEAD
viability staining experiments, can be one of the specific mechanisms
behind the antibacterial action. L. monocytogenes was particularly sensitive to pinosylvin, and this effect was also seen in L. monocytogenes
internalized in intestinal Caco2 cells at non-cytotoxic pinosylvin
concentrations. In general, the antimicrobial effects of pinosylvin were
even more prominent than those of a related stilbene, resveratrol, well
known for its various bioactivities. According to our results,
pinosylvin could have potential as a natural disinfectant or biocide in
some targeted applications.
Original language | English |
---|---|
Pages (from-to) | 99-107 |
Number of pages | 9 |
Journal | International Journal of Food Microbiology |
Volume | 164 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2013 |
MoE publication type | A1 Journal article-refereed |
Keywords
- wood associated phenolic compounds
- stilbenes
- pinosylvin
- Salmonella
- Listeria monocytogenes
- Food spoilage