The application of antibodies in immunosensing

Martin Albers, Janusz Sadowski, Harri Joki, Sinikka Airikkala, Inger Vikholm, Jukka Lekkala

Research output: Book/ReportReport

Abstract

This report presents some of the results of the BETA project concerning immobilization of antibodies on sensor materials. An antibody against human Gorionic Gonadotropin (anti-hCG) was immobilized on glass, quartz, silicon and different metallic surfaces. Kinetic measurements of binding of anti-hCG and hCG to different metals were carried out with the surface plasmon resonance (SPR) technique. Immobilization of the antibody, covalently on the different surfaces, was done either by epoxylation or by aminosilanization, followed by glutaraldehyde activation. The binding constants of the antibody were compared with these of the same antibody adsorbed on polystyrene. The activity of the antibody, found on the solid surfaces, was sufficiently high for use in conventional immunoassays (RIA and IRMA). It was also concluded that immunosensing, with SPR, is feasible. However, the coating methods should be still further developed to improve the detection sensitivity and to suppress non-specific binding and crossreactions. Also the detection sensitivity of the used SPR equipment should be enhanced.
Original languageEnglish
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Number of pages112
ISBN (Print)951-38-3611-8
Publication statusPublished - 1989
MoE publication typeD4 Published development or research report or study

Publication series

SeriesValtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports
Number670
ISSN1235-0613

Fingerprint

Antibodies
Surface plasmon resonance
Gonadotropins
Quartz
Polystyrenes
Glutaral
Silicon
Metals
Chemical activation
Glass
Coatings
Kinetics
Sensors

Keywords

  • biomedical measurement
  • sensors
  • biosensors
  • immunology
  • immobilization
  • antibodies

Cite this

Albers, M., Sadowski, J., Joki, H., Airikkala, S., Vikholm, I., & Lekkala, J. (1989). The application of antibodies in immunosensing. Espoo: VTT Technical Research Centre of Finland. Valtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports, No. 670
Albers, Martin ; Sadowski, Janusz ; Joki, Harri ; Airikkala, Sinikka ; Vikholm, Inger ; Lekkala, Jukka. / The application of antibodies in immunosensing. Espoo : VTT Technical Research Centre of Finland, 1989. 112 p. (Valtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports; No. 670).
@book{12d55f042d9643c3bfbae1baf9bd8dfe,
title = "The application of antibodies in immunosensing",
abstract = "This report presents some of the results of the BETA project concerning immobilization of antibodies on sensor materials. An antibody against human Gorionic Gonadotropin (anti-hCG) was immobilized on glass, quartz, silicon and different metallic surfaces. Kinetic measurements of binding of anti-hCG and hCG to different metals were carried out with the surface plasmon resonance (SPR) technique. Immobilization of the antibody, covalently on the different surfaces, was done either by epoxylation or by aminosilanization, followed by glutaraldehyde activation. The binding constants of the antibody were compared with these of the same antibody adsorbed on polystyrene. The activity of the antibody, found on the solid surfaces, was sufficiently high for use in conventional immunoassays (RIA and IRMA). It was also concluded that immunosensing, with SPR, is feasible. However, the coating methods should be still further developed to improve the detection sensitivity and to suppress non-specific binding and crossreactions. Also the detection sensitivity of the used SPR equipment should be enhanced.",
keywords = "biomedical measurement, sensors, biosensors, immunology, immobilization, antibodies",
author = "Martin Albers and Janusz Sadowski and Harri Joki and Sinikka Airikkala and Inger Vikholm and Jukka Lekkala",
year = "1989",
language = "English",
isbn = "951-38-3611-8",
series = "Valtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports",
publisher = "VTT Technical Research Centre of Finland",
number = "670",
address = "Finland",

}

Albers, M, Sadowski, J, Joki, H, Airikkala, S, Vikholm, I & Lekkala, J 1989, The application of antibodies in immunosensing. Valtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports, no. 670, VTT Technical Research Centre of Finland, Espoo.

The application of antibodies in immunosensing. / Albers, Martin; Sadowski, Janusz; Joki, Harri; Airikkala, Sinikka; Vikholm, Inger; Lekkala, Jukka.

Espoo : VTT Technical Research Centre of Finland, 1989. 112 p. (Valtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports; No. 670).

Research output: Book/ReportReport

TY - BOOK

T1 - The application of antibodies in immunosensing

AU - Albers, Martin

AU - Sadowski, Janusz

AU - Joki, Harri

AU - Airikkala, Sinikka

AU - Vikholm, Inger

AU - Lekkala, Jukka

PY - 1989

Y1 - 1989

N2 - This report presents some of the results of the BETA project concerning immobilization of antibodies on sensor materials. An antibody against human Gorionic Gonadotropin (anti-hCG) was immobilized on glass, quartz, silicon and different metallic surfaces. Kinetic measurements of binding of anti-hCG and hCG to different metals were carried out with the surface plasmon resonance (SPR) technique. Immobilization of the antibody, covalently on the different surfaces, was done either by epoxylation or by aminosilanization, followed by glutaraldehyde activation. The binding constants of the antibody were compared with these of the same antibody adsorbed on polystyrene. The activity of the antibody, found on the solid surfaces, was sufficiently high for use in conventional immunoassays (RIA and IRMA). It was also concluded that immunosensing, with SPR, is feasible. However, the coating methods should be still further developed to improve the detection sensitivity and to suppress non-specific binding and crossreactions. Also the detection sensitivity of the used SPR equipment should be enhanced.

AB - This report presents some of the results of the BETA project concerning immobilization of antibodies on sensor materials. An antibody against human Gorionic Gonadotropin (anti-hCG) was immobilized on glass, quartz, silicon and different metallic surfaces. Kinetic measurements of binding of anti-hCG and hCG to different metals were carried out with the surface plasmon resonance (SPR) technique. Immobilization of the antibody, covalently on the different surfaces, was done either by epoxylation or by aminosilanization, followed by glutaraldehyde activation. The binding constants of the antibody were compared with these of the same antibody adsorbed on polystyrene. The activity of the antibody, found on the solid surfaces, was sufficiently high for use in conventional immunoassays (RIA and IRMA). It was also concluded that immunosensing, with SPR, is feasible. However, the coating methods should be still further developed to improve the detection sensitivity and to suppress non-specific binding and crossreactions. Also the detection sensitivity of the used SPR equipment should be enhanced.

KW - biomedical measurement

KW - sensors

KW - biosensors

KW - immunology

KW - immobilization

KW - antibodies

M3 - Report

SN - 951-38-3611-8

T3 - Valtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports

BT - The application of antibodies in immunosensing

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Albers M, Sadowski J, Joki H, Airikkala S, Vikholm I, Lekkala J. The application of antibodies in immunosensing. Espoo: VTT Technical Research Centre of Finland, 1989. 112 p. (Valtion teknillinen tutkimuskeskus. Tutkimuksia - Research Reports; No. 670).