The application of recombinant-DNA technology to cellulases and lignocellulosic wastes

Jonathan Knowles, Päivi Lehtovaara, Tuula Teeri, Merja Penttilä, Irma Salovuori, Lars André

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    The efficient utilization of different lignocellulosic wastes will require an understanding of the roles of the different enzymes involved in enzymatic hydrolysis. Towards this goal, we have isolated and characterized the genes coding for four major cellulases, CBH I, CBH II, EG I and EG III, produced by the cellulolytic fungus Trichoderma reesei. It seems that T. reesei produces at least two classes of cellulose and in each class both endo- and exo-type enzyme are found. Full-length cDNAs coding for CBH I, CBH II, EG I and EG III have been expressed in the yeast Saccharomyces cerevisiae, and the cellulases are secreted by these strains. The recombinant cellulases produced by the yeast all show activity towards cellulosic substrates. The information obtained from the cloned genes makes possible the construction of new cellulolytic organisms and may also make possible the development of improved enzymes.
    Original languageEnglish
    Pages (from-to)449-454
    JournalPhilosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
    Volume321
    Issue number1561
    DOIs
    Publication statusPublished - 1987
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Dive into the research topics of 'The application of recombinant-DNA technology to cellulases and lignocellulosic wastes'. Together they form a unique fingerprint.

    Cite this