The application of recombinant-DNA technology to cellulases and lignocellulosic wastes

Jonathan Knowles; Päivi Lehtovaara; Tuula Teeri; Merja Penttilä; Irma Salovuori; Lars André

doi:10.1098/rsta.1987.0022

The application of recombinant-DNA technology to cellulases and lignocellulosic wastes

Jonathan Knowles, Päivi Lehtovaara, Tuula Teeri, Merja Penttilä, Irma Salovuori, Lars André

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

The efficient utilization of different lignocellulosic wastes will require an understanding of the roles of the different enzymes involved in enzymatic hydrolysis. Towards this goal, we have isolated and characterized the genes coding for four major cellulases, CBH I, CBH II, EG I and EG III, produced by the cellulolytic fungus Trichoderma reesei. It seems that T. reesei produces at least two classes of cellulose and in each class both endo- and exo-type enzyme are found. Full-length cDNAs coding for CBH I, CBH II, EG I and EG III have been expressed in the yeast Saccharomyces cerevisiae, and the cellulases are secreted by these strains. The recombinant cellulases produced by the yeast all show activity towards cellulosic substrates. The information obtained from the cloned genes makes possible the construction of new cellulolytic organisms and may also make possible the development of improved enzymes.

Original language	English
Pages (from-to)	449-454
Journal	Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
Volume	321
Issue number	1561
DOIs	https://doi.org/10.1098/rsta.1987.0022
Publication status	Published - 1987
MoE publication type	A1 Journal article-refereed

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10.1098/rsta.1987.0022

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title = "The application of recombinant-DNA technology to cellulases and lignocellulosic wastes",

abstract = "The efficient utilization of different lignocellulosic wastes will require an understanding of the roles of the different enzymes involved in enzymatic hydrolysis. Towards this goal, we have isolated and characterized the genes coding for four major cellulases, CBH I, CBH II, EG I and EG III, produced by the cellulolytic fungus Trichoderma reesei. It seems that T. reesei produces at least two classes of cellulose and in each class both endo- and exo-type enzyme are found. Full-length cDNAs coding for CBH I, CBH II, EG I and EG III have been expressed in the yeast Saccharomyces cerevisiae, and the cellulases are secreted by these strains. The recombinant cellulases produced by the yeast all show activity towards cellulosic substrates. The information obtained from the cloned genes makes possible the construction of new cellulolytic organisms and may also make possible the development of improved enzymes.",

author = "Jonathan Knowles and P{\"a}ivi Lehtovaara and Tuula Teeri and Merja Penttil{\"a} and Irma Salovuori and Lars Andr{\'e}",

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doi = "10.1098/rsta.1987.0022",

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journal = "Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences",

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The application of recombinant-DNA technology to cellulases and lignocellulosic wastes. / Knowles, Jonathan; Lehtovaara, Päivi; Teeri, Tuula; Penttilä, Merja; Salovuori, Irma; André, Lars.

In: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 321, No. 1561, 1987, p. 449-454.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - The application of recombinant-DNA technology to cellulases and lignocellulosic wastes

AU - Knowles, Jonathan

AU - Lehtovaara, Päivi

AU - Teeri, Tuula

AU - Penttilä, Merja

AU - Salovuori, Irma

AU - André, Lars

PY - 1987

Y1 - 1987

N2 - The efficient utilization of different lignocellulosic wastes will require an understanding of the roles of the different enzymes involved in enzymatic hydrolysis. Towards this goal, we have isolated and characterized the genes coding for four major cellulases, CBH I, CBH II, EG I and EG III, produced by the cellulolytic fungus Trichoderma reesei. It seems that T. reesei produces at least two classes of cellulose and in each class both endo- and exo-type enzyme are found. Full-length cDNAs coding for CBH I, CBH II, EG I and EG III have been expressed in the yeast Saccharomyces cerevisiae, and the cellulases are secreted by these strains. The recombinant cellulases produced by the yeast all show activity towards cellulosic substrates. The information obtained from the cloned genes makes possible the construction of new cellulolytic organisms and may also make possible the development of improved enzymes.

AB - The efficient utilization of different lignocellulosic wastes will require an understanding of the roles of the different enzymes involved in enzymatic hydrolysis. Towards this goal, we have isolated and characterized the genes coding for four major cellulases, CBH I, CBH II, EG I and EG III, produced by the cellulolytic fungus Trichoderma reesei. It seems that T. reesei produces at least two classes of cellulose and in each class both endo- and exo-type enzyme are found. Full-length cDNAs coding for CBH I, CBH II, EG I and EG III have been expressed in the yeast Saccharomyces cerevisiae, and the cellulases are secreted by these strains. The recombinant cellulases produced by the yeast all show activity towards cellulosic substrates. The information obtained from the cloned genes makes possible the construction of new cellulolytic organisms and may also make possible the development of improved enzymes.

U2 - 10.1098/rsta.1987.0022

DO - 10.1098/rsta.1987.0022

M3 - Article

VL - 321

SP - 449

EP - 454

JO - Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences

JF - Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences

SN - 1364-503X

IS - 1561

ER -

The application of recombinant-DNA technology to cellulases and lignocellulosic wastes

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