The arabinolytic pathway in Trichoderma reesei

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

One of the most widespread pentose sugars found in plant biomass is L-arabinose, a constituent of plant cell-wall polysaccharides L-arabinan, arabinogalactans and arabinoxylans. The pentose catabolic pathways are relevant for micro-organisms living on decaying plant material but also in biotechnology when cheap raw materials such as plant hydrolysates are used as a carbon source. Genes coding for both extracellular and intracellular enzymes of the arabinolytic pathway have been cloned from the industrially important fungusTrichoderma reesei. The fungal intracellular L-arabinose pathway consists of five enzymes, aldose reductase, L-arabinitol 4-dehydrogenase, L-xylulose reductase, xylitol dehydrogenase and xylulokinase. All the genes coding for the enzymes of this pathway have been cloned in our laboratory. These genes have been utilized to construct an arabinose-utilizing S. cerevisiae strain. We have investigated the expression of the extracellular arabinofuranosidase that cleaves arabinose form arabinoxylans and intracellular arabinolytic genes in T. reesei cells grown on various carbon sources and the regulation of these genes with respect to the carbon catabolite repressor CREI.
Original languageEnglish
Title of host publicationPosters Abstracts
Publication statusPublished - 2004
Event7th European Conference on Fungal Genetics - Copenhagen, Denmark
Duration: 17 Apr 200420 Apr 2004

Conference

Conference7th European Conference on Fungal Genetics
CountryDenmark
CityCopenhagen
Period17/04/0420/04/04

Fingerprint

Trichoderma reesei
arabinose
arabinoxylan
pentoses
genes
carbon
arabinitol
enzymes
aldehyde reductase
xylulose
alpha-N-arabinofuranosidase
arabinogalactans
xylitol
hydrolysates
biotechnology
raw materials
polysaccharides
cell walls
metabolites
sugars

Cite this

Aro, N., Verho, R., & Penttilä, M. (2004). The arabinolytic pathway in Trichoderma reesei. In Posters Abstracts [Vp-1]
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abstract = "One of the most widespread pentose sugars found in plant biomass is L-arabinose, a constituent of plant cell-wall polysaccharides L-arabinan, arabinogalactans and arabinoxylans. The pentose catabolic pathways are relevant for micro-organisms living on decaying plant material but also in biotechnology when cheap raw materials such as plant hydrolysates are used as a carbon source. Genes coding for both extracellular and intracellular enzymes of the arabinolytic pathway have been cloned from the industrially important fungusTrichoderma reesei. The fungal intracellular L-arabinose pathway consists of five enzymes, aldose reductase, L-arabinitol 4-dehydrogenase, L-xylulose reductase, xylitol dehydrogenase and xylulokinase. All the genes coding for the enzymes of this pathway have been cloned in our laboratory. These genes have been utilized to construct an arabinose-utilizing S. cerevisiae strain. We have investigated the expression of the extracellular arabinofuranosidase that cleaves arabinose form arabinoxylans and intracellular arabinolytic genes in T. reesei cells grown on various carbon sources and the regulation of these genes with respect to the carbon catabolite repressor CREI.",
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Aro, N, Verho, R & Penttilä, M 2004, The arabinolytic pathway in Trichoderma reesei. in Posters Abstracts., Vp-1, 7th European Conference on Fungal Genetics, Copenhagen, Denmark, 17/04/04.

The arabinolytic pathway in Trichoderma reesei. / Aro, Nina; Verho, Ritva; Penttilä, Merja.

Posters Abstracts. 2004. Vp-1.

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - The arabinolytic pathway in Trichoderma reesei

AU - Aro, Nina

AU - Verho, Ritva

AU - Penttilä, Merja

N1 - Poster presentation

PY - 2004

Y1 - 2004

N2 - One of the most widespread pentose sugars found in plant biomass is L-arabinose, a constituent of plant cell-wall polysaccharides L-arabinan, arabinogalactans and arabinoxylans. The pentose catabolic pathways are relevant for micro-organisms living on decaying plant material but also in biotechnology when cheap raw materials such as plant hydrolysates are used as a carbon source. Genes coding for both extracellular and intracellular enzymes of the arabinolytic pathway have been cloned from the industrially important fungusTrichoderma reesei. The fungal intracellular L-arabinose pathway consists of five enzymes, aldose reductase, L-arabinitol 4-dehydrogenase, L-xylulose reductase, xylitol dehydrogenase and xylulokinase. All the genes coding for the enzymes of this pathway have been cloned in our laboratory. These genes have been utilized to construct an arabinose-utilizing S. cerevisiae strain. We have investigated the expression of the extracellular arabinofuranosidase that cleaves arabinose form arabinoxylans and intracellular arabinolytic genes in T. reesei cells grown on various carbon sources and the regulation of these genes with respect to the carbon catabolite repressor CREI.

AB - One of the most widespread pentose sugars found in plant biomass is L-arabinose, a constituent of plant cell-wall polysaccharides L-arabinan, arabinogalactans and arabinoxylans. The pentose catabolic pathways are relevant for micro-organisms living on decaying plant material but also in biotechnology when cheap raw materials such as plant hydrolysates are used as a carbon source. Genes coding for both extracellular and intracellular enzymes of the arabinolytic pathway have been cloned from the industrially important fungusTrichoderma reesei. The fungal intracellular L-arabinose pathway consists of five enzymes, aldose reductase, L-arabinitol 4-dehydrogenase, L-xylulose reductase, xylitol dehydrogenase and xylulokinase. All the genes coding for the enzymes of this pathway have been cloned in our laboratory. These genes have been utilized to construct an arabinose-utilizing S. cerevisiae strain. We have investigated the expression of the extracellular arabinofuranosidase that cleaves arabinose form arabinoxylans and intracellular arabinolytic genes in T. reesei cells grown on various carbon sources and the regulation of these genes with respect to the carbon catabolite repressor CREI.

M3 - Conference abstract in proceedings

BT - Posters Abstracts

ER -

Aro N, Verho R, Penttilä M. The arabinolytic pathway in Trichoderma reesei. In Posters Abstracts. 2004. Vp-1